Phospholipid Analysis by Nanoflow Liquid Chromatography-Tandem Mass Spectrometry

Moon, Myeong Hee

doi:10.5478/msl.2014.5.1.1

Cited by 8 publications

(3 citation statements)

References 47 publications

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“…There are a vast number of papers devoted to the analysis of phospholipid, e.g. by mass spectrometric techniques . However, to the best of our knowledge, formation of complexes between phospholipids and any organic compounds has not been demonstrated yet.…”

Section: Methodsmentioning

confidence: 99%

Complexation of phosphates by 1,3-bis(3-(2-pyridylureido)propyl)-1,1,3,3-tetramethyldisiloxane

Frański

Gierczyk

Markiewicz

et al. 2015

Rapid Commun. Mass Spectrom.

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Section: Methodsmentioning

confidence: 99%

Complexation of phosphates by 1,3-bis(3-(2-pyridylureido)propyl)-1,1,3,3-tetramethyldisiloxane

Frański

Gierczyk

Markiewicz

et al. 2015

Rapid Commun. Mass Spectrom.

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“…When sample throughput is not an issue, very long columns or even two-dimensional LC can be applied; both approaches can improve the separation efficiency substantially. On the other hand, nanoLC can be used for specific purposes-for example, if the amount of sample is very limited [51]. Normal-phase LC and hydrophilic interaction LC are used less frequently in clinical applications; their advantage is that both modes typically distinguish lipid species according to their hydrophilic functionalities, so lipids are separated according to their representative polar head group classes.…”

Section: Lc-ms-based Lipidomicsmentioning

confidence: 99%

Optimizing the lipidomics workflow for clinical studies—practical considerations

Hyötyläinen

Orešič

2015

Anal Bioanal Chem

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Lipidomics is increasingly being used in clinical research, offering new opportunities for disease prediction and detection. One of the key challenges of clinical applications of lipidomics is the high sensitivity of measured lipid levels to many analytical, physiological, and environmental factors, which therefore must be taken into account when designing the studies. Here we critically discuss the complete clinical lipidomics workflow, including selection of the subjects, the sample type, the sample preprocessing conditions, and the analytical method and methods for data processing. We also review the lipidomics applications which investigate the confounding factors such as age, gender, fasting time, and handling procedures for measuring blood lipid metabolites.

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“…Combining the techniques of liquid chromatography (LC) with MS via electrospray ionization (ESI) has expanded the range of analytical performance in identifying as many lipids as possible, owing to the decrease in ionization suppression from the highly abundant species. − Recently, lipidomic analysis with LC-MS has been attempted to investigate lipid profiles in patient plasma or urine samples in various diseases, such as colorectal cancer, breast cancer, , diabetes, prostate cancer, , and Gaucher’s disease . Specifically, the use of nanoflow LC using capillary columns, prior to MS, is effective in acquiring an enhanced resolution of separation and also improves the sensitivity of detection at low levels (up to femtomolar levels) of lipid species, , owing to the reduced consumption of LC solvents. Lipids have been recognized as important signaling molecules that regulate biological processes not only in normal but also in diseased conditions .…”

Section: Introductionmentioning

confidence: 99%

Lipidomic Perturbations in Lung, Kidney, and Liver Tissues of p53 Knockout Mice Analyzed by Nanoflow UPLC-ESI-MS/MS

et al. 2016

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Lipids are important signaling molecules regulating biological processes under normal and diseased conditions. Although p53 mutation is well-known for causing cancer, the relationship between p53-related tumorigenesis and altered lipid profile is unclear. We profiled differences in lipid expressions in liver, lung, and kidney in p53 knockout (KO) mice by high-speed quantitative analysis of 320 lipids (399 species identified) using nanoflow ultrahigh performance liquid chromatography−tandem mass spectrometry (nUPLC-MS/MS). Lung tissues were most severely affected by the lack of p53 gene, as shown by significant reduction (24−44%, P < 0.05) in total phosphatidylcholine (PC), phosphatidylethanolamine (PE), sphingomyelin (SM), diacylglycerol (DG), and triacylglycerol (TG), and significant increases (30−50%) in phosphatidylserine (PS), phosphatidylinositol (PI), and monohexosylceramide (MHC). MHC levels increased in all tissues. Dihexosylceramide (DHC) level decreased only in kidney tissue. Most PI, PS, and phosphatidic acid (PA) species showing significant increases contained a saturated acyl chain (18:0) in lung and liver tissues. Neutral glycerolipids (16:0/22:0-DG and most TGs with saturated and monounsaturated acyl chains) decreased 2−4-fold in the liver tissue. Our results suggest that the lack of p53 and altered lipid profiles are closely related, but as their changes vary from one tissue to another, the lipid alterations are tissue-specific.

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Phospholipid Analysis by Nanoflow Liquid Chromatography-Tandem Mass Spectrometry

Cited by 8 publications

References 47 publications

Complexation of phosphates by 1,3-bis(3-(2-pyridylureido)propyl)-1,1,3,3-tetramethyldisiloxane

Complexation of phosphates by 1,3-bis(3-(2-pyridylureido)propyl)-1,1,3,3-tetramethyldisiloxane

Optimizing the lipidomics workflow for clinical studies—practical considerations

Lipidomic Perturbations in Lung, Kidney, and Liver Tissues of p53 Knockout Mice Analyzed by Nanoflow UPLC-ESI-MS/MS

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