Phospholipid-protein balance in affective disorders: Analysis of human blood serum using Raman and FTIR spectroscopy. A pilot study

Depciuch, Joanna; Sowa-Kućma, Magdalena; Nowak, Gabriel; Dudek, Dominika; Siwek, Marcin; Styczeń, Krzysztof; Parlińska-Wojtan, Magdalena

doi:10.1016/j.jpba.2016.08.037

Cited by 47 publications

(36 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although the cross section of Raman scattering is low, it is extremely sensitive to intramolecular conditions. In addition, although water is always present as one of the elements of the biological materials, it does not affect the Raman spectroscopic fingerprint of biomolecules too much …”

Section: Discussionmentioning

confidence: 99%

“…While proteoglycan 4, hyaluronic acid (HA), and surface active phospholipids (SAPL) contribute to the total arrangement of the system . Walker et al termed this combination of trapped pool and fluid concentration as “boosted lubrication.” The proteins are produced from synovium, cartilage, ligament, and meniscus, and their structural and functional properties are directly connected with the phospholipids . On the other hand, HA contributes more as a potential boundary lubricant for cartilage and shows a small amount of lubrication activity …”

Section: Biochemical and Mechanical Properties Of Sf And Joint Replacmentioning

confidence: 99%

“…Pursuing biochemical reactions underlying the formation of the lubricating film, vibrational fingerprint provided by Raman spectroscopy can be used to identify the components present and analyse the chemical bonding among them . In addition, the correlation between the level of phospholipids and proteins exists and can be deduced from Raman spectra . The technique has also been previously used to distinguish between healthy SF and SF changes in osteoarthritis (OA) patients as well as to OA subchondral bone matrix change detection …”

Section: Introductionmentioning

confidence: 99%

“…10 In addition, the correlation between the level of phospholipids and proteins exists and can be deduced from Raman spectra. 11 The technique has also been previously used to distinguish between healthy SF and SF changes in osteoarthritis (OA) patients as well as to OA subchondral bone matrix change detection. 10,12 The objectives of this study are to review and clarify the chemical reactions of SF components during the formation of the lubricating film on the joint replacement along with the focus on mechanical properties of the film.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A systematic review on correlation between biochemical and mechanical processes of lubricant film formation in joint replacement of the last 10 years

Rufaqua

Vrbka

Choudhury

et al. 2019

Lubrication Science

View full text Add to dashboard Cite

This study provides a systematic review of the synovial and simulated body fluid research for the last 10 years (2006 to 2016). In particular, biochemical and mechanical properties of synovial fluid after joint replacement are focused, namely, the chemical composition of the formed lubricating film and structural changes of the associated proteins under mechanical loading. In summary, the formation of the film depends on pH, viscosity, and concentration of the solution, static, sliding, and rolling conditions and other factors related to the joint replacement. However, chemical changes of the synovial fluid proteins after the joint replacement are rarely addressed and require further attention. To this end, we provide a preliminary study of selected proteins within the synovial fluid using Raman spectroscopy. We conclude that chemical analysis together with the analysis of mechanical and biological properties of the synovial fluid after total joint replacement will help in comprehension of the process.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Biochemical and Mechanical Properties Of Sf And Joint Replacmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A systematic review on correlation between biochemical and mechanical processes of lubricant film formation in joint replacement of the last 10 years

Rufaqua

Vrbka

Choudhury

et al. 2019

Lubrication Science

View full text Add to dashboard Cite

show abstract

“…analyzed the behavior of platelet proteins through Raman spectroscopy as a noninvasive method for the diagnosis of Alzheimer's disease. This spectroscopy method, in addition to FTIR‐ATR spectroscopy, has been proposed for the analysis of serum samples in order to identify markers of depressive disorders . Therefore, we employed a similar strategy to analyze the spectra of healthy and hypertensive platelets.…”

Section: Discussionmentioning

confidence: 99%

Analysis of platelets in hypertensive and normotensive individuals using Raman and Fourier transform infrared‐attenuated total reflectance spectroscopies

García-Rubio

Mora²,

Badillo‐Ramírez

et al. 2019

J Raman Spectroscopy

View full text Add to dashboard Cite

Platelets of both healthy and hypertensive subjects were analyzed by Raman and Fourier transform infrared by attenuated total reflectance (FTIR‐ATR) spectroscopies. We compared the average relative intensities of the main Raman peaks, the areas of convoluted bands in the amide I region, and the second derivative of the FTIR‐ATR spectra. Key differences were found in the bands reflecting lipid content and protein structure. The Raman spectra exhibited statistically significant changes in the intensity of bands associated with CC stretching vibrations in the carbon chains of lipids (960 cm−1) and the amide I band (centered at 1,658 cm−1). The amide I deconvolution showed changes in the area percentages of the bands corresponding to different protein secondary structures, suggesting biochemical and protein conformational differences between healthy versus arterial hypertension platelets, which might be related to the platelet activation stage. An analysis by using the second derivative of the FTIR‐ATR spectra, followed by deconvolution of amide regions support this observation, revealing differences in the amide II and amide I bands. Moreover, modifications observed in the phosphate‐associated bands are possibly related to the phospholipids' behavior and the phosphorylation of proteins. Our results suggest interesting differences between the spectra of healthy versus hypertensive platelets, which may be mainly associated with biochemical changes at the cellular membrane level.

show abstract

An efficient primary screening of COVID‐19 by serum Raman spectroscopy

Yin

Lü

et al. 2021

J Raman Spectroscopy

View full text Add to dashboard Cite

The outbreak of COVID‐19 coronavirus disease around the end of 2019 has become a pandemic. The preferred method for COVID‐19 detection is the real‐time polymerase chain reaction (RT‐PCR)‐based technique; however, it also has certain limitations, such as sample‐dependent procedures with a relatively high false negative ratio. We propose a safe and efficient method for screening COVID‐19 based on Raman spectroscopy. A total of 177 serum samples are collected from 63 confirmed COVID‐19 patients, 59 suspected cases, and 55 healthy individuals as a control group. Raman spectroscopy is adopted to analyze these samples, and a machine learning support‐vector machine (SVM) method is applied to the spectrum dataset to build a diagnostic algorithm. Furthermore, 20 independent individuals, including 5 asymptomatic COVID‐19 patients and 5 symptomatic COVID‐19 patients, 5 suspected patients, and 5 healthy patients, were sampled for external validation. In these three groups—confirmed COVID‐19, suspected, and healthy individuals—the distribution of statistically significant points of difference showed highly consistency for intergroups after repeated sampling processes. The classification accuracy between the COVID‐19 cases and the suspected cases is 0.87 (95% confidence interval [CI]: 0.85–0.88), and the accuracy between the COVID‐19 and the healthy controls is 0.90 (95% CI: 0.89–0.91), while the accuracy between the suspected cases and the healthy control group is 0.68 (95% CI: 0.67–0.73). For the independent test dataset, we apply the obtained SVM model to the classification of the independent test dataset to have all the results correctly classified. Our model showed that the serum‐level classification results were all correct for independent test dataset. Our results suggest that Raman spectroscopy could be a safe and efficient technique for COVID‐19 screening.

show abstract

Phospholipid-protein balance in affective disorders: Analysis of human blood serum using Raman and FTIR spectroscopy. A pilot study

Cited by 47 publications

References 33 publications

A systematic review on correlation between biochemical and mechanical processes of lubricant film formation in joint replacement of the last 10 years

A systematic review on correlation between biochemical and mechanical processes of lubricant film formation in joint replacement of the last 10 years

Analysis of platelets in hypertensive and normotensive individuals using Raman and Fourier transform infrared‐attenuated total reflectance spectroscopies

An efficient primary screening of COVID‐19 by serum Raman spectroscopy

Contact Info

Product

Resources

About