Photo-crosslinked GelMA/collagen membrane loaded with lysozyme as an antibacterial corneal implant

Yang, Xiangjing; Sun, Xiaomin; Liu, Jia; Huang, Yongrui; Peng, Yuehai; Xu, Yingni; Li, Ren

doi:10.1016/j.ijbiomac.2021.09.144

Cited by 23 publications

(16 citation statements)

References 36 publications

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“… Ztaou et al (2016) found that bilateral activation of indirect pathway MSNs by optogenetics can produce Parkinson’s-like presentation. However, activation of MSNs in the direct pathway alleviates symptoms such as freezing, bradykinesia, and difficulty in initiating movement ( Kravitz et al, 2010 ; de Almeida and da Silva, 2021 ; Malaquias et al, 2021 ; Yang X. et al, 2021 ). Yang et al (2018) found that the use of optogenetics combined with deep brain stimulation (DBS) ( Castano-Candamil et al, 2019 ) to stimulate the afferent axons of the subthalamic nucleus region at high frequency can significantly treat Parkinson’s disease ( Table 1 and Figure 3 ).…”

Section: Nervous System-based Clinical Researchmentioning

confidence: 99%

The Roles of Optogenetics and Technology in Neurobiology: A Review

Chen

Liang

et al. 2022

Front. Aging Neurosci.

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Optogenetic is a technique that combines optics and genetics to control specific neurons. This technique usually uses adenoviruses that encode photosensitive protein. The adenovirus may concentrate in a specific neural region. By shining light on the target nerve region, the photosensitive protein encoded by the adenovirus is controlled. Photosensitive proteins controlled by light can selectively allow ions inside and outside the cell membrane to pass through, resulting in inhibition or activation effects. Due to the high precision and minimally invasive, optogenetics has achieved good results in many fields, especially in the field of neuron functions and neural circuits. Significant advances have also been made in the study of many clinical diseases. This review focuses on the research of optogenetics in the field of neurobiology. These include how to use optogenetics to control nerve cells, study neural circuits, and treat diseases by changing the state of neurons. We hoped that this review will give a comprehensive understanding of the progress of optogenetics in the field of neurobiology.

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Section: Nervous System-based Clinical Researchmentioning

confidence: 99%

The Roles of Optogenetics and Technology in Neurobiology: A Review

Chen

Liang

et al. 2022

Front. Aging Neurosci.

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“…Staphylococcus aureus ( S. aureus ) is a common disease-causing bacteria, which can lead to local purulent infection once the wound forms, thereby even life-threatening in severe cases [ 3 , 4 ]. Currently, to address this issue, numerous types of wound dressings, such as membranes, sponge, aerogel, scaffold, and hydrogels have been developed [ [5] , [6] , [7] ]. Among them, the hydrogel was widely used in accelerating wound healing, due to its three-dimensionally porous structure, high hydroscopicity and water holding property, good biodegradability and biocompatibility, and was similar to the natural extracellular matrix (ECM) [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of gelatin-based and Zn2+-incorporated composite hydrogel for accelerated infected wound healing

Tao

Lin

Qin³

et al. 2022

Materials Today Bio

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“…Biomaterials should have sufficient stability against collagenase degradation to provide an environment for cells as scaffolds. 14 For our CCRMs, ideally, they can provide an environment for cell growth, so that human corneal epithelial cells (HCECs) can heal quickly and isolate the external environment in the early stage. Under the degradation of the CCRMs, the corneal stromal cells grow into the materials and secrete extracellular matrix to reshape the cornea.…”

Section: Results and Discussionmentioning

confidence: 99%

“…The properties of native cornea are mainly provided by the corneal stroma (mainly composed of collagen type I), which accounts for 90% of corneal thickness. , Collagen type I is the main component of corneal stromal layer, , so collagen as corneal regeneration material has incomparable advantages over other natural polymer materials. Since 2018, our research group has successfully prepared collagen-based corneal repair materials (CCRMs) for corneal lamellar transplantation by different methods. − Meanwhile, control of microorganisms on the CCRMs is quite important for their performance during the in vitro cell experiments and in vivo animal studies. Irradiation is often used to control the microorganisms in the material, but which type of radiation source and what doses can effectively control the microorganisms on CCRMs without affecting their properties and the effect on macrophage polarization have not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Radiation Sources on the Performance of Collagen-Based Corneal Repair Materials and Macrophage Polarization

et al. 2022

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Owing to the lack of donor corneas, there is an urgent need for suitable corneal substitutes. As the main component of the corneal stroma, collagen has great advantages as a corneal repair material. If there are microorganisms such as bacteria in the corneal repair material, it may induce postoperative infection, causing the failure of corneal transplantation. Therefore, irradiation, as a common sterilization method, is often used to control the microorganisms in the material. However, it has not been reported which type of radiation source and what doses can sterilize more effectively without affecting the properties of collagen-based corneal repair materials (CCRMs) and have a positive impact on macrophage polarization. In this study, three different radiation sources of ultraviolet, cobalt-60, and electron beam at four different doses of 2, 5, 8, and 10 kGy were used to irradiate CCRMs. The swelling, stretching, transmittance, and degradation of the irradiated CCRMs were characterized, and the proliferation of human corneal epithelial cells on the irradiated CCRMs was characterized using the CCK8 kit. The results showed that low dose (<5 kGy) of radiation had little effect on the performance of CCRMs. Three irradiation methods with less influence were selected for the further study on RAW264.7 macrophage polarization. The results indicated that CCRMs treated with UV could downregulate the expression of pro-inflammatory related genes and upregulate the expression of anti-inflammatory genes in macrophages, which indicated that UV irradiation is a beneficial process for the preparation of CCRMs.

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Photo-crosslinked GelMA/collagen membrane loaded with lysozyme as an antibacterial corneal implant

Cited by 23 publications

References 36 publications

The Roles of Optogenetics and Technology in Neurobiology: A Review

The Roles of Optogenetics and Technology in Neurobiology: A Review

Fabrication of gelatin-based and Zn2+-incorporated composite hydrogel for accelerated infected wound healing

Effects of Different Radiation Sources on the Performance of Collagen-Based Corneal Repair Materials and Macrophage Polarization

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