Photo-cured hyaluronic acid-based hydrogels containing growth and differentiation factor 5 (GDF-5) for bone tissue regeneration

Bae, Min Soo; Ohe, Joo-Young; Lee, Jung Bok; Heo, Dong Nyoung; Byun, Wook; Bae, Hojae; Kwon, Yong‐Dae; Kwon, Il Keun

doi:10.1016/j.bone.2013.11.019

Cited by 88 publications

(47 citation statements)

References 39 publications

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“…Photopolymerization has been widely employed to crosslink hydrogels due to its rapid and mild reactions with minimal cytotoxicity and possible in situ polymerization. 4–12 …”

Section: Introductionmentioning

confidence: 99%

“…13, 14 In contrast to current photocuring systems using UV light 12, 15 , our hydrogel could be prepared under mild conditions, reducing potential adverse effects associated with UV exposure and toxic initiators. Although the hydrogel system supported proliferation and extracellular matrix (ECM) deposition of encapsulated mesenchymal stem cells 16–18 , lack of specific cell binding domains on chitosan surfaces and their highly hydrophilic nature with resultant low protein adsorption can limit cell-matrix interactions, leading to poor osteogenic cellular responses 16, 19 .…”

Section: Introductionmentioning

confidence: 99%

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Photocrosslinkable chitosan hydrogels functionalized with the RGD peptide and phosphoserine to enhance osteogenesis

et al. 2016

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Hydrogels derived from naturally occurring polymers are attractive matrix for tissue engineering. Here, we report a biofunctional hydrogel for specific use in bone regeneration by introducing Arg-Gly-Asp (RGD)-containing cell adhesive motifs and phosphorylated serine residues, which are prevalent in native bone extracellular matrix and known to promote osteogenesis by enhancing cell-matrix interactions and hydroxyapatite nucleation, into photopolymerizable methacrylated glycol chitosan (MeGC). Incorporation of phosphoserine into MeGC hydrogels increased the ability of the hydrogels to nucleate mineral on their surfaces. RGD incorporation enhanced cell-matrix interactions by supporting attachment, spreading, and proliferation of bone marrow stromal cells (BMSCs) encapsulated in the hydrogels. Moreover, co-modification of MeGC hydrogels with RGD and phosphoserine synergistically increased osteogenic differentiation of encapsulated BMSCs in vitro. The bone healing capacity of the modified hydrogels was further confirmed in a mouse calvarial defect model. These findings suggest a promising hydrogel platform with a specific microenvironment tailored to promote osteogenesis for clinical bone repair.

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“…Photopolymerization has been widely employed to crosslink hydrogels due to its rapid and mild reactions with minimal cytotoxicity and possible in situ polymerization. 4–12 …”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Photocrosslinkable chitosan hydrogels functionalized with the RGD peptide and phosphoserine to enhance osteogenesis

et al. 2016

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“…Injectable HA biomaterial had been developed and shown to promote bone formation in a minimally invasive subperiosteal model in rat calvaria . In addition, photo‐cured HA carrying growth and differentiation factor 5 or simvastatin could significantly improve osteogenesis . Glycidyl methacrylate‐modified HA hydrogels were designed to degrade at fast, intermediate, and slow rates.…”

Section: Effect Of Ha On Bone Formation In Vivomentioning

confidence: 99%

Effect of hyaluronic acid in bone formation and its applications in dentistry

Zhao

Wang

Qin

et al. 2016

J Biomedical Materials Res

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Hyaluronic acid (HA), the simplest glycosaminoglycan, participates in several important biological procedures, including mediation of cellular signaling, regulation of cell adhesion and proliferation, and manipulation of cell differentiation. The effect of HA on cell proliferation and differentiation depends on its molecular weight (MW) and concentration. Moreover, the properties of high viscosity, elasticity, highly negative charge, biocompatibility, biodegradability, and nonimmunogenicity make HA attractive in tissue engineering and disease treatment. This review comprises an overview of the effect of HA on cell proliferation and differentiation in vitro, the role of HA in bone regeneration in vivo, and the clinical applications of HA in dentistry, focusing on the mechanism underlining the effect of MW and concentration of HA on cell proliferation and osteogenic differentiation. It is expected that practical progress of HA both in laboratory-based experiments and clinical applications will be achieved in the next few years. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1560-1569, 2016.

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“…Hydrogels, which are just like living tissues when they are highly swollen in water, are in favor of the adhesion, proliferation and differentiation of cells, facilitate nutrient input and waste output, and provide enough space and mechanical stability for new tissue formation (Palumbo et al, 2014;Levengood & Zhang, 2014;Wang & Stegemann, 2010). Up to now, hydrogels have been extensively explored as scaffolds for tissue engineering such as bone (Bae et al, 2011(Bae et al, , 2014Slaughter, Khurshid, Fisher, Khademhosseini, & Peppas, 2009), cartilage (Levett et al, 2014), skin (Iannitti, Bingol, Rottigni, & Palmieri, 2013) and adipose (Tan et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Hyaluronic acid hydrogel scaffolds with a triple degradation behavior for bone tissue engineering

Cui

Qian

Liu

et al. 2015

Carbohydrate Polymers

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Photo-cured hyaluronic acid-based hydrogels containing growth and differentiation factor 5 (GDF-5) for bone tissue regeneration

Cited by 88 publications

References 39 publications

Photocrosslinkable chitosan hydrogels functionalized with the RGD peptide and phosphoserine to enhance osteogenesis

Photocrosslinkable chitosan hydrogels functionalized with the RGD peptide and phosphoserine to enhance osteogenesis

Effect of hyaluronic acid in bone formation and its applications in dentistry

Hyaluronic acid hydrogel scaffolds with a triple degradation behavior for bone tissue engineering

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