Photoacoustic and magnetic resonance imaging-based gene and photothermal therapy using mesoporous nanoagents

Abstract

The integration of photothermal therapy (PTT) with gene therapy (GT) in a single nanoscale platform demonstrates great potential in cancer therapy. Porous iron oxide nanoagents (PIONs) are widely used as magnetic nanoagents in the drug delivery field and also serve as a photothermal nanoagent for photothermal therapy. However, the therapeutic efficacy of PIONs-mediated GT has not been studied. The long noncoding RNA (lncRNA) CRYBG3 (LNC CRYBG3), a lncRNA induced by heavy ion irradiation in lung cancer cells, has been reported to directly bind to globular actin (G-actin) and cause degradation of cytoskeleton and blocking of cytokinesis, thus indicating its potential for use in GT by simulating the effect of heavy ion irradiation and functioning as an antitumor drug. In the present study, we investigated the possibility of combining PIONs-mediated PTT and LNC CRYBG3-mediated GT to destroy non-small cell lung cancer (NSCLC) cells both in vitro and in vivo . The combination therapy showed a high cancer cell killing efficacy, and the cure rate was better than that achieved using PTT or GT alone. Moreover, as a type of magnetic nanoagent, PIONs can be used for magnetic resonance imaging (MRI) and photoacoustic imaging (PAI) both in vitro and in vivo . These findings indicate that the new combination therapy has high potential for cancer treatment.

Nontoxic In Vivo Clearable Nanoparticle Clusters for Theranostic Applications

“…The photothermal conversion efficiency (PCE) of IONs and GIONs was calculated to be ∼23 and ∼51%, respectively, indicating significant contribution from the coupled surface plasmon resonance of GSs. The PCE of GIONs is considerably significant as compared to the existing iron oxide/gold nanoparticle-based photothermal agents. − Furthermore, magnetic properties of IONs and GIONs were recorded at 300 K, showing a saturation magnetization of ∼88 and ∼80 emu/g, respectively (Figure G). The significant saturation magnetization of IONs is in agreement with a previous report on larger magnetic clusters with a crystallite/grain size of about 10 nm .…”

The tumor therapeutic potential of long non-coding RNA delivery and targeting

“…LncRNA CRYBG3 , which is induced by ion irradiation, was identified as a tumor suppressor by promoting apoptosis. By combining the treatment of PIONs- CRYBG3 and ion irradiation, researchers realized the best treatment of lung cancer in vitro and in vivo 82 . The combination of radiotherapy enhanced the expression of CRYBG3 in tumor cells, and additionally, it also increased the delivery efficiency of PIONs- CRYBG3 by locally heating the tissues ( Fig.…”

“… LncRNA Role Carrier Disease Ref. LOC2836367 Target Lentiviral vector (LV) Inflammation-related diseases 42 DANCR Target Lentiviral vector (LV) Hepatocellular carcinoma (HCC) 43 LINC01257 Target Lipid nanoparticle (LNP) Acute myeloid leukemia (AML) 61 MALAT1 Target ASO-Au-TAT nanoparticle Lung cancer 62 NEAT1 Drug Extracellular vesicles (EVs) Cardiac fibrosis 68 H19 Drug Extracellular vesicle-mimetic nanovesicles (EMNVs) Diabetic chronic wounds 75 MEG3 Drug Virus-like particles (VLPs) Hepatocellular carcinoma (HCC) 77 MEG3 Drug Supramolecular complex Hepatocellular carcinoma (HCC) 79 CRYBG3 Drug Porous iron oxide nanoagents (PIONs) Lung cancer 82 …”

Application of Nanozymes in Biomedical Imaging

“…[69] Fe 3 O 4 not only possesses the properties of nanozymes but has also been reported to possess high-quality MR imaging performance. [70] An Fe 3 O 4 -based nanozyme (Fe 3 O 4 @Cu 1.77 Se) was fabricated for MR imaging and cancer therapy. [71] Fe 3 O 4 @Cu 1.77 Se remains [71] (e) Preparation of CMGCC nanoclusters.…”