Photobleached-fluorescence imaging of microflows

Mosier, Bruce P.; Molho, Joshua I.; Santiago, Juan G.

doi:10.1007/s00348-002-0486-8

Cited by 61 publications

(55 citation statements)

References 34 publications

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“…[32][33][34][35] The most successful method that applies the traditional advanced anemometer, is micro Particle Image Velocimetry (mPIV), [36][37][38] but it is difficult to apply to commercial instruments. Photobleached Fluorescence Visualization [39][40][41] and the line writing technique [42][43][44] with photobleaching are also developed to measure the flow velocity. However, all the methods mentioned above have limited temporal resolution.…”

Section: Introductionmentioning

confidence: 99%

Laser induced fluorescence photobleaching anemometer for microfluidic devices

Wang

2005

Lab Chip

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We have developed a novel, non-intrusive fluid velocity measurement method based on photobleaching of a fluorescent dye for microfluidic devices. The residence time of the fluorescent dye in a laser beam depends on the flow velocity and approximately corresponds to the decaying time of the photobleaching of the dye in the laser beam. The residence time is inversely proportional to the flow velocity. The fluorescence intensity increases with the flow velocity due to the decrease of the residence time. A calibration curve between fluorescence intensity and known flow velocity should be obtained first. The calibration relationship is then used to calculate the flow velocity directly from the measured fluorescence intensity signal. The new method can measure the velocity very quickly and is easy to use. It is demonstrated for both pressure driven flow and electroosmotic flow.

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Section: Introductionmentioning

confidence: 99%

Laser induced fluorescence photobleaching anemometer for microfluidic devices

Wang

2005

Lab Chip

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“…This can be achieved using photobleachedfluorescence imaging velocimetry. 17 This technique has been used previously by the authors to study the flow of PB in steelglass 18 and glass-glass EHD point contacts. 19 The throughthickness velocity profile, , have been obtained successfully.…”

Section: Introductionmentioning

confidence: 99%

The effect of boundary slip on elastohydrodynamic lubrication

Ponjavic

Wong

2014

RSC Adv.

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“…Photobleached-fluorescence imaging (where the fluorescence of a dilute and neutral fluorophore is suppressed by exposing it to visible excitation light) has also been developed for monitoring EOF [32][33][34][35], but this technique has not yet been applied to characterize surface modifications, although it offers the advantage of using a very low concentration of marker, and will work with both glass and polymer microchannels, which are transparent to visible light.…”

Section: Spatially Resolved Eof Measurementsmentioning

confidence: 99%

Surface treatment and characterization: Perspectives to electrophoresis and lab‐on‐chips

et al. 2006

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The control and modification of surface state is a major challenge in bioanalytical sciences, and in particular in electrokinetic separation methods, due to the importance of electroosmosis. This topic has gained recently a renewed interest, associated with the development of "lab-on-chips" systems that extend the range of materials in which separation channels are fabricated. The surface science community has developed through the years a large toolbox of characterization tools and surface modification protocols, which is not yet fully exploited in the bioanalytical world. In this paper, we try and present an overview of these tools, in order to stimulate new ideas for improved and more controlled surface treatment strategies for separations in capillaries and microchannels. We briefly describe some physical and chemical aspects of electroosmosis (global and spatially resolved), streaming current, and streaming potential. We also review the main strategies for surface coating, and compare the advantages of physisorption, well-organized thin self-assembled monolayers, or conversely thick polymer "brushes". Examples of existing applications to electrophoresis in microchannel are also given.

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Photobleached-fluorescence imaging of microflows

Cited by 61 publications

References 34 publications

Laser induced fluorescence photobleaching anemometer for microfluidic devices

Laser induced fluorescence photobleaching anemometer for microfluidic devices

The effect of boundary slip on elastohydrodynamic lubrication

Surface treatment and characterization: Perspectives to electrophoresis and lab‐on‐chips

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