Photochemical internalization for pDNA transfection: Evaluation of poly(d,l-lactide-co-glycolide) and poly(ethylenimine) nanoparticles

Gargouri, Myriem; Sapin, Anne; Arıca-Yegin, Betül; Merlin, Jean Louis; Bécuwe, Philippe; Maincent, Philippe

doi:10.1016/j.ijpharm.2010.10.040

Cited by 16 publications

(9 citation statements)

References 47 publications

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“…The nanocomposite size is of importance, as it influences the cellular uptake behavior and the clearance from blood stream by the mononuclear phagocytic system (MPS) in vivo 27, 28. Our data show that the solvent displacement method leads to smaller particles in comparison to earlier published results for PLGA nanoparticle preparation by double emulsion technique and adsorption of PEI/DNA‐polyplexes on the particle surface afterwards 29. Particles with larger diameters than presented here were also obtained in the case of preparation of folic acid‐decorated nanoparticles by a layer‐by‐layer technique 22.…”

Section: Resultsmentioning

confidence: 47%

Folic Acid‐Decorated Nanocomposites Prepared by a Simple Solvent Displacement Method

Benfer

Reul

Betz

et al. 2012

Macromolecular Bioscience

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A biodegradable nanocarrier system based on PLGA applicable for FR targeting is described. PEI-based conjugates with covalently coupled folic acid are synthesized, characterized with regard to their composition and used for DNA complexation. The preparation of composites is performed by a solvent displacement technique, assuming an electrostatic interaction of PEI-based polyplexes with PLGA. The synthesis of a folic acid-PEG3kDa-PEI25kDa conjugate is achieved. Blending of PLGA with polyplexes results in spherical nanoparticles with sizes ≤ 250 nm. Incorporation of polyplexes and the localization of folic acid on the particle surface, performed by antibody binding, is confirmed. The method is suitable for the preparation of nanosized, folic-acid-decorated nanoparticles.

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Section: Resultsmentioning

confidence: 47%

Folic Acid‐Decorated Nanocomposites Prepared by a Simple Solvent Displacement Method

Benfer

Reul

Betz

et al. 2012

Macromolecular Bioscience

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“…[ 72 ] . Briefl y, the NPs/pEGFP-ZNF580 complexes with various N/P molar ratios ranging from 10 to 30, containing pEGFP-ZNF580 (20 µg) in each Eppendorf tube, were incubated in Tris-HCl buffer (200 µL) (pH = 7.4) in a shaking incubator at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Nanoparticles Complexed with Gene Vectors to Promote Proliferation of Human Vascular Endothelial Cells

Shi

Zhang

et al. 2015

Adv Healthcare Materials

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Amphiphilic block copolymers containing biodegradable hydrophobic segments of depsipeptide based copolymers have been synthesized and explored as gene carriers for enhancing proliferation of endothelial cells in vitro. These polymers form nanoparticles (NPs) with positive charges on their surface, which could condense recombinant plasmids of enhanced green fluorescent protein plasmid and ZNF580 gene (pEGFP-ZNF580) and protect them against DNase I. ZNF580 gene is efficiently transported into EA.hy926 cells to promote their proliferation, whereby the transfection efficiency of NPs/pEGFP-ZNF580 is approximately similar to that of Lipofectamine 2000. These results indicate that the NPs might have potential as a carrier for pEGFP-ZNF580, which could support endothelialization of cardiovascular implants.

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“…Two important reaction factors that determine the structure difference of our LGA-PEI polymer with other polymers that include PLGA and PEI are the reaction time of PLGA with PEI and the PLGA to PEI ratio. Thus, our LGA-PEI polymer is a new material, which has differences in chemical structure, properties, functions and potential applications from those reported in literature [44,54,[65][66][67][68][69][70][71].…”

Section: Discussionmentioning

confidence: 95%

“…Several studies reported preparing PLGA NPs bearing PEI on their surface by mixing first the PLGA NP with the water-in-oilin-water technique in PVI solution, and then adding PEI into the solution to form a PEI-coated PLGA NPs [44,54,[65][66][67][68][69][70][71]. In these studies, PLGA NPs are the core, and PEI attached to the surface makes the NPs cationic.…”

Section: Discussionmentioning

confidence: 99%

New Polymer of lactic-co-glycolic acid-modified Polyethylenimine for Nucleic Acid Delivery

Lü

Liang

Wang

et al. 2016

Nanomedicine (Lond.)

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Aim:To develop an improved delivery system for nucleic acids. Materials & methods:We designed, synthesized and characterized a new polymer of lactic-co-glycolic acidmodified polyethylenimine (LGA-PEI). Functions of LGA-PEI polymer were determined. Results:The new LGA-PEI polymer spontaneously formed nanoparticles (NPs) with DNA or RNA, and showed higher DNA or RNA loading efficiency, higher or comparable transfection efficacy, and lower cytotoxicity in several cell types including PANC-1, Jurkat and HEK293 cells, when compared with lipofectamine 2000, branched or linear PEI (25 kDa). In nude mouse models, LGA-PEI showed higher delivery efficiency of plasmid DNA or miRNA mimic into pancreatic and ovarian xenograft tumors.LGA-PEI/DNA NPs showed much lower toxicity than control PEI NPs in mouse models. Conclusion:The new LGA-PEI polymer is a safer and more effective system to deliver DNA or RNA than PEI.

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Photochemical internalization for pDNA transfection: Evaluation of poly(d,l-lactide-co-glycolide) and poly(ethylenimine) nanoparticles

Cited by 16 publications

References 47 publications

Folic Acid‐Decorated Nanocomposites Prepared by a Simple Solvent Displacement Method

Folic Acid‐Decorated Nanocomposites Prepared by a Simple Solvent Displacement Method

Nanoparticles Complexed with Gene Vectors to Promote Proliferation of Human Vascular Endothelial Cells

New Polymer of lactic-co-glycolic acid-modified Polyethylenimine for Nucleic Acid Delivery

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