Photodynamic antibacterial enhanced effect of methylene blue-gold nanoparticles conjugate on Staphylococcal aureus isolated from impetigo lesions in vitro study

Tawfik, Abeer Attia; Alsharnoubi, Jehan; Morsy, Mona

doi:10.1016/j.pdpdt.2015.03.003

Cited by 31 publications

(25 citation statements)

References 10 publications

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“…The antimicrobial photodynamic inactivation of planktonic cells after light exposure was higher in TB MSN treated samples than in TB treated samples. Similar to previous literature, Gram-positive cells were more susceptible to aPDT treatment than Gram-negative cells because of the difference in their cell envelopes (Tawfik et al 2015;Usacheva et al 2016).…”

Section: Discussionsupporting

confidence: 88%

Antimicrobial photodynamic activity of toluidine blue encapsulated in mesoporous silica nanoparticles againstPseudomonas aeruginosaandStaphylococcus aureus

Parasuraman

Antony

et al. 2019

Biofouling

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In the present study, the antimicrobial and antibiofilm efficacy of toluidine blue (TB) encapsulated in mesoporous silica nanoparticles (MSN) was investigated against Pseudomonas aeruginosa and Staphylococcus aureus treated with antimicrobial photodynamic therapy (aPDT) using a red diode laser 670 nm wavelength, 97.65 J cm À2 radiant exposure, 5 min). Physico-chemical techniques (UV-visible (UV-vis) absorption, photoluminescence emission, excitation, and FTIR) and high-resolution transmission electron microscopy (HR-TEM) were employed to characterize the conjugate of TB encapsulated in MSN (TB MSN). TB MSN showed maximum antimicrobial activities corresponding to 5.03 and 5.56 log CFU ml À1 reductions against P. aeruginosa and S. aureus, respectively, whereas samples treated with TB alone showed 2.36 and 2.66 log CFU ml À1 reductions. Anti-biofilm studies confirmed that TB MSN effectively inhibits biofilm formation and production of extracellular polymeric substances by P. aeruginosa and S. aureus. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 88%

Antimicrobial photodynamic activity of toluidine blue encapsulated in mesoporous silica nanoparticles againstPseudomonas aeruginosaandStaphylococcus aureus

Parasuraman

Antony

et al. 2019

Biofouling

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“…In 2015, Morsy and co-workers [ 70 ] also reported that the presence of 13 nm AuNP could improve the phototoxicity of MB towards an S. aureus strain isolated from childrens’ impetigo lesions; this strain is the most common organism found in impetigo infections and resistance to conventional antibiotics starts to be problematic. The authors prepared the PS-AuNP nanoconjugates by using the swell–encapsulation–shrink method.…”

Section: Metal Nanoparticlesmentioning

confidence: 99%

Revisiting Current Photoactive Materials for Antimicrobial Photodynamic Therapy

et al. 2018

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Microbial infection is a severe concern, requiring the use of significant amounts of antimicrobials/biocides, not only in the hospital setting, but also in other environments. The increasing use of antimicrobial drugs and the rapid adaptability of microorganisms to these agents, have contributed to a sharp increase of antimicrobial resistance. It is obvious that the development of new strategies to combat planktonic and biofilm-embedded microorganisms is required. Photodynamic inactivation (PDI) is being recognized as an effective method to inactivate a broad spectrum of microorganisms, including those resistant to conventional antimicrobials. In the last few years, the development and biological assessment of new photosensitizers for PDI were accompanied by their immobilization in different supports having in mind the extension of the photodynamic principle to new applications, such as the disinfection of blood, water, and surfaces. In this review, we intended to cover a significant amount of recent work considering a diversity of photosensitizers and supports to achieve an effective photoinactivation. Special attention is devoted to the chemistry behind the preparation of the photomaterials by recurring to extensive examples, illustrating the design strategies. Additionally, we highlighted the biological challenges of each formulation expecting that the compiled information could motivate the development of other effective photoactive materials.

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“…The use of nanotechnology is an attractive approach to improving the effectiveness of APDI against resistant microbial cells [128–133] and to improve PS delivery to biofilms [134–136]. This goal may be achieved in different ways, for instance by enhancing the delivery of PS to microorganisms (encapsulating the PS in nanoparticles) [137] or by increasing the 1 O 2 yield of the PS (by covalently binding the PS to the surface of the nanoparticles [138], or simply by mixing the nanoparticles and PS together).…”

Section: Biofilm Resistance To Apdimentioning

confidence: 99%

Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Kashef

Hamblin

2017

Drug Resistance Updates

255

173

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Infections have been a major cause of disease throughout the history of humans on earth. With the introduction of antibiotics, it was thought that infections had been conquered. However, bacteria have been able to develop resistance to antibiotics at an exponentially increasing rate. The growing threat from multi-drug resistant organisms calls for intensive action to prevent the emergence of totally resistant and untreatable infections. Novel, non-invasive, non-antibiotic strategies are needed that act more efficiently and faster than current antibiotics. One promising alternative is antimicrobial photodynamic inactivation (APDI), an approach that produces reactive oxygen species when dyes and light are combined. So far, it has been questionable if bacteria can develop resistance against APDI. This review paper gives an overview of recent studies concerning the susceptibility of bacteria towards oxidative stress, and suggests possible mechanisms of the development of APDI-resistance that should at least be addressed. Some ways to potentiate APDI and also to overcome future resistance are suggested.

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Photodynamic antibacterial enhanced effect of methylene blue-gold nanoparticles conjugate on Staphylococcal aureus isolated from impetigo lesions in vitro study

Cited by 31 publications

References 10 publications

Antimicrobial photodynamic activity of toluidine blue encapsulated in mesoporous silica nanoparticles againstPseudomonas aeruginosaandStaphylococcus aureus

Antimicrobial photodynamic activity of toluidine blue encapsulated in mesoporous silica nanoparticles againstPseudomonas aeruginosaandStaphylococcus aureus

Revisiting Current Photoactive Materials for Antimicrobial Photodynamic Therapy

Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

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