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Low-level laser therapy (LLLT) has been used in photobiomodulation to promote healing, regenerate, and restore tissue function. Women with persistent thin endometrium were assumed to encounter diminished activity in the regenerative and functional capacity of their endometrium. The aim of this study was to examine the ability of LLLT in 635 nm wavelength to enhance the proliferation and gene expression of in vitro cultured endometrial cells. Single (SE) versus multiple exposures (ME) to LLLT were examined in the study groups and compared to controls. A fluence dose of 4.27 J/cm for 16 min was given once in the SE or divided in three equal sessions in days 2, 4, and 6 of the culture in ME. Cellular response was evaluated by measuring viable cell numbers and surface area. Pattern of MUC1, ITGA5, ITGB3, LIF, and PTEN gene expression was assessed using the qRT-PCR. Greater numbers of cells were found in both study groups (P < 0.001) as compared to controls. The surface area of cells at the end of culture phase was highly significant (P < 0.001) in ME when compared to SE and controls. A statistically significant difference was found in terms of gene expression in both irradiated groups (P < 0.001) as compared to controls, although greater difference in PTEN tumour suppressor gene (Phosphatase and tensin homolog) expression was toward ME. The introduction of LLLT to the armamentarium of infertility will serve as a new adjuvant therapy in this field. The current study proofed that LLLT was able to increase the proliferative and functional capacity of cultured endometrial cells.

The laser pretreatment of seed is drawing pronounced attention from the scientific community for its positive impact in boosting germination, seedling, and growth of plants. In this study, the laser pretreatment of Adansonia digitata (A. digitata) seeds was evaluated. Eight laser treatments were conducted at different powers, 10, 20, 40, and 80 mW, with the two-time interval for each power at 2 and 4 min. The outcomes indicated that the most efficient irradiation was 10 mW/2 min which induces the highest germination rate and polyphenolic contents for seeds. Based on these results, the animal experimental design was processed to assess the hepatoprotective activity of A. digitata extracts obtained through the optimum laser preillumination to enhance the resistance of liver damage in mice. The total phenol and flavonoid contents and the antioxidant properties of the methanolic extracts were estimated in vitro. The CCl4 was used to induce hepatotoxicity in mice. The animals were divided into five groups. The sera of the treated animals were used for the determination of transaminases, and the liver homogenates were used for the determination of antioxidant status, and further liver tissues were subjected to verify the anti-apoptotic effect of A. digitata methanolic extract. The in vivo results showed that the methanolic extract exposed to laser treatment at 10 mW/2 min provided better hepatoprotective capacity than the other treatments. Administration of A. digitata extract not only offered a significant decrease in liver enzyme activity but also markedly improved the antioxidant status and reduced the apoptotic progression induced by CCl4 toxicity in liver tissue.

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Background: Osteoarthritis (OA) is one of the most prevalent causes of musculoskeletal pain and disability among the middle age and elderly population. It significantly affects the overall quality of life of the affected individuals. Objectives: To find out the impact of high intensity laser therapy (HILT) on myofascial trigger points (MTrPs) in symptomatic knee OA. Methods: This is a single-blinded pre–post randomized experimental trial. A total of 40 patients, both male and female, falling within the age group of 35-55 years, BMI 25–29.9 Kg/Cm2, diagnosed with bilateral knee osteoarthritis (grade 2), were recruited as participants of the study. All the participants were clinically evaluated for the signs and symptoms of myofascial pain, tenderness over the vastus medialis muscle and the medial head of the gastrocnemius muscle. The participants were randomly allocated into two groups with n=20 in each group. The participants of the experimental group received high-power laser therapy and underwent exercises on the MTrPS of the vastus medialis and the medial head of the gastrocnemius muscle. The participants of the control group received placebo laser "sham laser" and underwent exercises for the vastus medialis and the medial head of the gastrocnemius muscle. A blood cortisol level test, pressure algometer, digital electronic goniometer, and the KOOS-PS questionnaire were used to measure the pain level, pain threshold, knee ROM, and knee function. Outcome assessment was done at Day 0 and post intervention at the end of 4 weeks. Results: The MANOVA test revealed that both groups improved significantly in all parameters at week 4, with the HILT group improving more than the sham group in blood cortisol levels, pressure algometer measures, knee passive ROM, and KOOS scores (P < .05). Conclusion: HILT is an efficient approach in the treatment of MTrPs in knee OA.