Photodynamic antimicrobial chemotherapy action of phenothiazinium dyes in planktonic Candida albicans is increased in sodium dodecyl sulfate

Collina, Gabriela Alves da; Freire, Fernanda; Barbosa, Vinicius da Silva; Correa, Caroline Bento; Nascimento, Helenyce Reis do; Horliana, Anna Carolina Ratto Tempestini; Silva, Daniela de Fátima Teixeira da; Prates, Renato Araújo; Pavani, Christiane

doi:10.1016/j.pdpdt.2019.101612

Cited by 14 publications

(10 citation statements)

References 53 publications

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“…It is widely known that the aPDT community lacks on standards for in vitro aPDT assays and that any changes in inocula concentration, photosensitizer concentration, solvent composition (e.g., water, PBS, saline solution, etc. ), light irradiance, optical path, and other factors may lead to diverging results [61][62][63]. Therefore, it is very unlikely that precise comparisons of inactivation kinetics data are achievable between two studies performed by different teams who varied any of the above-mentioned parameters and analyzed a reduced spectrum of species.…”

Section: Discussionmentioning

confidence: 99%

Global priority multidrug-resistant pathogens do not resist photodynamic therapy

Sabino¹,

Wainwright²,

Ribeiro³

et al. 2020

Journal of Photochemistry and Photobiology B: Biology

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Microbial drug-resistance demands immediate implementation of novel therapeutic strategies. Antimicrobial photodynamic therapy (aPDT) combines the administration of a photosensitizer (PS) compound with low-irradiance light to induce photochemical reactions that yield reactive oxygen species (ROS).Since ROS react with nearly all biomolecules, aPDT offers a powerful multitarget method to avoid selection of drug-resistant strains. In this study, we assayed photodynamic inactivation under a standardized method, combining methylene blue (MB) as PS and red light, against global priority pathogens. The species tested include Acinetobacter baumannii, Klebsiella aerogenes, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, Candida albicans and Cryptococcus neoformans. Our strain collection presents resistance to all tested antimicrobials (>50). All drug-resistant strains were compared to their drug-sensitive counterparts. Regardless of resistance phenotype, MB-aPDT presented species-specific dose-response kinetics.More than 5log10 reduction was observed within less than 75 seconds of illumination for A. baumannii, E. coli, E. faecium, E. faecalis and S. aureus and within less than 7 minutes for K. aerogenes, K. pneumoniae, P. aeruginosa, C. albicans and C. neoformans. No signs of cross-resistance or -tolerance in between aPDT and standard chemotherapy was observed. Therefore, MB-aPDT can provide effective therapeutic protocols for a very broad spectrum of pathogens. Hence, we believe that this study represents a very important step to bring aPDT closer to implementation into mainstream medical practices.

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Section: Discussionmentioning

confidence: 99%

Global priority multidrug-resistant pathogens do not resist photodynamic therapy

Sabino¹,

Wainwright²,

Ribeiro³

et al. 2020

Journal of Photochemistry and Photobiology B: Biology

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“…The synergistic effects of PACT with other surfactant agents have been well studied mainly in the field of periodontal diseases. Sodium dodecyl sulfate, a surfactant agent, increased the PAE of methylene blue on Porphyromonas gingivalis [23] and of azure A, azure B, and dimethyl methylene blue on Candida albicans [24]. Moreover, to the best of our knowledge, no report has addressed the synergistic effect of BAC and PACT mediated by any photosensitizer.…”

Section: Discussionmentioning

confidence: 99%

Synergistic effect of TONS504-mediated photodynamic antimicrobial chemotherapy and additives widely contained in ophthalmic solutions: benzalkonium chloride and ethylenediaminetetraacetic acid

Shinji

Chikama

Okazaki

et al. 2022

Photochem Photobiol Sci

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TONS504 (C 51 H 58 N 8 O 5 I 2 ), a chlorine derivative, effectively generates singlet oxygen by light activation and exhibits photodynamic antimicrobial effects (PAEs) on various pathogens. However, this photosensitizer has some limitations: a high tendency to self-aggregate and a relatively weak PAE for Gram-negative bacteria compared with Gram-positive bacteria. To overcome these limitations, the present study investigated the synergistic effects of the PAE of TONS504 and two additives commonly contained in ophthalmic solutions: benzalkonium chloride (BAC) or ethylenediaminetetraacetic acid (EDTA). Staphylococcus aureus and Pseudomonas aeruginosa were exposed to TONS504 and/or each additive. Photodynamic antimicrobial chemotherapy was performed with light irradiation centered at a wavelength of 665 nm with a total light energy of 30 J/cm 2 . Following incubation, the number of colonies formed was counted. Additionally, we examined the inhibitory effects of the additives on TONS504 self-aggregation by observing its absorption spectrum. Consequently, the PAEs of TONS504 on S. aureus were enhanced by both additives, and BAC displayed stronger synergistic effects on the bacteria than EDTA. By contrast, only EDTA increased the PAE on P. aeruginosa. The peak of the TONS504 absorption spectrum shifted to a longer wave length and the absorbance increased in the presence of BAC, suggesting that BAC inhibited the self-aggregation of the photosensitizer. In conclusion, the combination of BAC or EDTA and TONS504-mediated photodynamic antimicrobial chemotherapy exhibits a synergistic antimicrobial effect on S. aureus and P. aeruginosa. The optimal additive to enhance the PAE may differ between bacterial strains.

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“…Antimicrobial photodynamic therapy (APDT), also known as photodynamic antimicrobial chemotherapy (PACT), was used in periodontal treatment based on scaling and root planing, as an adjunct form to decrease the bacterial load in the periodontal pocket and avoid using systemic antimicrobials (1)(2)(3). APDT for periodontitis consists of the topical administration of a photosensitizer (PS) that is distributed within the periodontal pockets with accumulation on microbial biofilms.…”

Section: Introductionmentioning

confidence: 99%

“…The aggregation state determines the types of photochemical reactions that occur, and these affect the efficiency of treatment with APDT. The presence of dimers or monomers is noticeable through the visible absorption spectrum, with the dimer showing absorption at 590 nm and the monomer at 665 nm (3,7). The dimer / monomer ratio is commonly used to characterize the degree of aggregation of methylene blue in a given medium (8)(9)(10)(11).The application of MB in a solution of 0.25% w/v sodium dodecyl sulfate (SDS, surfactant vehicle) in phosphate-buffered saline (PBS), is intended to increase killing of the Gramnegative bacteria A. actinomycetemcomitans and P. gingivalis when compared to methylene blue in phosphate-buffered saline (PBS).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial photodynamic therapy mediated by methylene blue in surfactant vehicle on periodontopathogens

Tortamano

Anselmo

Kassa

et al. 2020

Photodiagnosis and Photodynamic Therapy

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Background: Periodontal disease (PD) is a chronic inflammatory disease caused by the presence of microbial biofilm. The aim of this study was to evaluate antimicrobial effect of antimicrobial photodynamic therapy (APDT) mediated by methylene blue (MB) in monomer form on A. actinomycetemcomitans and P. gingivalis.Methods: A. actinomycetemcomitans ATCC 29523 and P. gingivalis ATCC 33577 were cultured on anaerobic jars at 37°C for 48 h, and we tested APDT in the presence of 0.25% sodium dodecyl sulfate (SDS) in phosphate-buffered saline (PBS) or in PBS alone. APDT was carried out with 100 µM MB under laser radiation (PhotolaseIII, DMC, Brazil) at ʎ = 660 nm and parameters as following (P = 100 mW; I = 250 mW/cm 2 , and doses of 15, 45 and 75 J/cm 2 ).Results: Following APDT, PBS groups of A. actinomycetemcomitans presented 4 Logs of microbial death after 5 min irradiation. However, there was no bacterial reduction in SDS groups. On the other hand, P. gingivalis was sensitive to APDT in the presence of 0.25% SDS with 2 logs reduction from dark toxicity. Conclusion:The presence of 0.25% SDS can lead to different responses depending on the different microbial species.

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Photodynamic antimicrobial chemotherapy action of phenothiazinium dyes in planktonic Candida albicans is increased in sodium dodecyl sulfate

Cited by 14 publications

References 53 publications

Global priority multidrug-resistant pathogens do not resist photodynamic therapy

Global priority multidrug-resistant pathogens do not resist photodynamic therapy

Synergistic effect of TONS504-mediated photodynamic antimicrobial chemotherapy and additives widely contained in ophthalmic solutions: benzalkonium chloride and ethylenediaminetetraacetic acid

Antimicrobial photodynamic therapy mediated by methylene blue in surfactant vehicle on periodontopathogens

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