Photodynamic inactivation of
            <i>Lasiodiplodia theobromae</i>
            : lighting the way towards an environmentally friendly phytosanitary treatment

Garcia, Maíra Terra; David, Bruna Araújo; Sierra-García, Isabel N.; Faustino, Maria A. F.; Alves, Artur; Esteves, Ana Cristina

doi:10.1098/rsbl.2020.0820

Cited by 8 publications

(2 citation statements)

References 35 publications

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“…This therapy was also used successfully against phytopathogenic fungi such as Lasiodiplodia theobromae (causes vine trunk disease), Botrytis cinerea (causes plant necrosis), and Colletotrichum sp. (causes anthracnose in various fruit trees) [ 26 , 27 , 28 ]. As already mentioned, spores are crucial to spreading fungal diseases, and fungal conidia are relevant targets for photosensitization [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Photoinactivation of Fusarium oxysporum Conidia with Light-Activated Ammonium Phthalocyanines

Gamelas

Sierra-García

Tomé

et al. 2023

IJMS

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Antimicrobial photodynamic therapy (aPDT) has been explored as an innovative therapeutic approach because it can be used to inactivate a variety of microbial forms (vegetative forms and spores) without causing significant damage to host tissues, and without the development of resistance to the photosensitization process. This study assesses the photodynamic antifungal/sporicidal activity of tetra- and octasubstituted phthalocyanine (Pc) dyes with ammonium groups. Tetra- and octasubstituted zinc(II) phthalocyanines (1 and 2) were prepared and tested as photosensitizers (PSs) on Fusarium oxysporum conidia. Photoinactivation (PDI) tests were conducted with photosensitizer (PS) concentrations of 20, 40, and 60 µM under white-light exposure at an irradiance of 135 mW·cm–2, applied during 30 and 60 min (light doses of 243 and 486 J·cm−2). High PDI efficiency corresponding to the inactivation process until the detection limit was observed for both PSs. The tetrasubstituted PS was the most effective, requiring the lowest concentration and the shortest irradiation time for the complete inactivation of conidia (40 µM, 30 min, 243 J·cm−2). Complete inactivation was also achieved with PS 2, but a longer irradiation time and a higher concentration (60 µM, 60 min, 486 J·cm−2) were necessary. Because of the low concentrations and moderate energy doses required to inactivate resistant biological forms such as fungal conidia, these phthalocyanines can be considered potent antifungal photodynamic drugs.

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Section: Introductionmentioning

confidence: 99%

In Vitro Photoinactivation of Fusarium oxysporum Conidia with Light-Activated Ammonium Phthalocyanines

Gamelas

Sierra-García

Tomé

et al. 2023

IJMS

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“…PDI has shown to be effective against bacterial diseases, such as kiwifruit cancer [30] and citrus cancer [31], as well as phytopathogenic fungi such as Lasiodiplodia theobromae (the causative agent of vine trunk disease), Botrytis cinerea (associated with plant necrosis), and Colletotrichum sp. (responsible for anthracnose in various fruit trees) [32][33][34]. Fungal spores play a critical role in spreading fungal diseases, with fungal conidia being a significant target for photosensitization [35].…”

Section: Introductionmentioning

confidence: 99%

Light-Driven Tetra- and Octa-β-substituted Cationic Zinc(II) Phthalocyanines for Eradicating Fusarium oxysporum Conidia

Lourenço,

Cunha,

Sierra-Garcia

2023

IJMS

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Photodynamic inactivation (PDI) is an emerging therapeutic approach that can effectively inactivate diverse microbial forms, including vegetative forms and spores, while preserving host tissues and avoiding the development of resistance to the photosensitization procedure. This study evaluates the antifungal and sporicidal photodynamic activity of two water-soluble amphiphilic tetra- and octa-β-substituted zinc(II) phthalocyanine (ZnPc) dyes with dimethylaminopyridinium groups at the periphery (ZnPcs 1, 2) and their quaternized derivatives (ZnPcs 1a, 2a). Tetra(1, 1a)- and octa(2, 2a)-β-substituted zinc(II) phthalocyanines were prepared and assessed as photosensitizers (PSs) for their effects on Fusarium oxysporum conidia. Antimicrobial photoinactivation experiments were performed with each PS at 0.1, 1, 10, and 20 µM under white light irradiation at an irradiance of 135 mW·cm–2, for 60 min (light dose of 486 J·cm−2). High PDI efficiency was observed for PSs 1a, 2, and 2a (10 µM), corresponding to inactivation until the method’s detection limit. PS 1 (20 µM) also achieved a considerable reduction of >5 log10 in the concentration of viable conidia. The quaternized PSs (1a, 2a) showed better PDI performance than the non-quaternized ones (1, 2), even at the low concentration of 1 µM, and a light dose of 486 J·cm−2. These cationic phthalocyanines are potent photodynamic drugs for antifungal applications due to their ability to effectively inactivate resistant forms, like conidia, with low concentrations and reasonable energy doses.

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Photodynamic control of citrus crop diseases

Ferreira

Sierra-García

Guieu

et al. 2021

World J Microbiol Biotechnol

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Photodynamic inactivation of Lasiodiplodia theobromae : lighting the way towards an environmentally friendly phytosanitary treatment

Cited by 8 publications

References 35 publications

In Vitro Photoinactivation of Fusarium oxysporum Conidia with Light-Activated Ammonium Phthalocyanines

In Vitro Photoinactivation of Fusarium oxysporum Conidia with Light-Activated Ammonium Phthalocyanines

Light-Driven Tetra- and Octa-β-substituted Cationic Zinc(II) Phthalocyanines for Eradicating Fusarium oxysporum Conidia

Photodynamic control of citrus crop diseases

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