Photodynamic Modulation of Adrenergic Receptors in the Isolated Rat Hepatocytes

Selective activation by photodynamic action of cholecystokinin receptor in the freshly isolated rat pancreatic acini

“…For the latter case, SALPC photodynamic desensitization of adrenergic receptors may be a case in point. It was suggested that His–His, His–Lys crosslinks formed between transmembrane domains in the same receptor or between different receptors would confer spatial restraints on the receptor; therefore receptor activation was inhibited (Cui et al ., 2000). This may be similar to the inhibitory effect of disulphide bond formation on muscarinic receptor activation (Zeng et al ., 1999).…”

“…PDT is largely a type II photodynamic action involving the generation of singlet oxygen; singlet oxygen then triggers different cellular responses (Cui & Matthews, 1998). Previously, it has been found that photodynamic action could trigger smooth muscle contraction (Matthews & Mesler, 1984), stimulate amylase secretion in freshly isolated rat pancreatic acini (Matthews & Cui, 1989, 1990a), inhibit basal amylase secretion in pancreatic tumour cells (Matthews & Cui, 1990b), block degranulation in peritoneal mast cells (Hashikura et al ., 2001), and desensitize adrenergic receptors in freshly isolated hepatocytes (Cui et al ., 2000). Most significantly, photodynamic action in rat pancreatic acini could trigger calcium oscillations, which were identical to secretagogue‐induced or physiological oscillations (Cui & Kanno, 1997; Cui et al ., 1997).…”

Photodynamic Physiology—Photonanomanipulations in Cellular Physiology with Protein Photosensitizers

“…Singlet oxygen generated in photodynamic action with the sulphonated aluminum phthalocyanine (SALPC), has been found to activate rather permanently the CCK1 receptor in rat pancreatic acinar cells (Cui and Kanno, 1997; An et al, 2003; Cui et al, 2012), but desensitizes the α1 adrenergic receptor in rat hepatocytes (Cui et al, 2000) and other G protein-coupled receptors (GPCR).…”

“…Due to such cytocidal effects of singlet oxygen, photodynamic action has been found to be effective in the clinical treatments of both cancers and non-malignant lesions (Kennedy et al, 1990; Bown et al, 2002; Mittra and Singerman, 2002; Brown et al, 2004; Szeimies et al, 2010; Agostinis et al, 2011; Bown, 2013; Huggett et al, 2014; Craig et al, 2015; Abrahamse and Hamblin, 2016; Liu et al, 2016; Newman, 2016). On the other hand, it has been found that controlled doses of singlet oxygen could modulate cellular signaling in different cell types such as glandular cells with proven high specificity (Matthews and Cui, 1989, 1990a,b; al-Laith et al, 1993; Cui and Kanno, 1997; Cui et al, 1997, 2000, 2003, 2012; Cui and Matthews, 1998; Hashikura et al, 2001; Cui and Guo, 2002a,b; An et al, 2003; Wang et al, 2003; Krammer and Verwanger, 2012; Bacellar et al, 2015). One particular noted case is the photodynamic activation of CCK1 receptors in rat pancreatic acinar cells.…”

Direct chemiluminescent imaging detection of Cu/Zn-superoxidase dismutase, glutathione peroxidase, carbonic anhydrase-III, and catalase in rat liver cytosol separated by native porous gradient polyacrylamide gel electrophoresis

“…Hepatocytes were isolated as reported previously [30]. Fed rats were killed by cervical dislocation, the portal vein was cannulated, and perfused with Ca 21 5.6, HEPES 10, BSA 2 g/L, MEM amino acid mixture (2 mL/100 mL), L-glutamine 2, sodium pyruvate 2; the pH was adjusted to 7.4 with 4 M NaOH.…”