Photoelectrochemical biosensor for microRNA detection based on a MoS2/g-C3N4/black TiO2 heterojunction with Histostar@AuNPs for signal amplification

Wang, Minghui; Yin, Huanshun; Zhou, Yunlei; Sui, Chengji; Wang, Yue; Xun, Meng; Waterhouse, Geoffrey I. N.; Ai, Shiyun

doi:10.1016/j.bios.2018.12.048

Cited by 112 publications

(45 citation statements)

References 48 publications

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“…In addition to ECL method, PEC detection method based on g‐C 3 N 4 has also attracted extensive attention because the excitation light source and electrical readout signal are part of different energy forms, endowing it with a low background signal and high sensitivity [93b,100] . For instance, Wang's group reported a novel PEC biosensor for miRNA detection based on a ternary MoS 2 /g‐C 3 N 4 /black TiO 2 heterojunction as the photoactive material, and the AuNPs carrying Histostar antibodies (Histostar@AuNPs) for signal amplification [101] . In this PEC biosensor, MoS 2 /g‐C 3 N 4 /black TiO 2 was first deposited on an indium tin oxide electrode surface, then AuNPs and probe DNA were assembled on the modified electrode.…”

Section: Carbon Nanomaterials For Microrna Electrochemical Biosensingmentioning

confidence: 99%

Recent Applications of Carbon Nanomaterials for microRNA Electrochemical Sensing

Wang

Wen

Yan

2020

Chemistry An Asian Journal

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MicroRNA (miRNA) is an important tumor marker in the human body, and its early detection has a great influence on the survival rate of patients. Although there are many detection methods for miRNA at present such as northern blotting, real‐time quantitative polymerase chain reaction, microarrays, and others, electrochemical biosensors have the advantages of low detection cost, small instrument size, simple operation, non‐invasive detection and low consumption of reagents and solvents, and thus they play an important role in the early detection of cancer. In addition, with the development of nanotechnology, nano‐biosensors show great potential. The application of various nanomaterials in the development of electrochemical biosensor has greatly improved the detection sensitivity of electrochemical biosensor. Among them, carbon nanomaterials which have unique electrical, optical, physical and chemical properties have attracted increasing attention. In particular, they have a large surface area, good biocompatibility and conductivity. Therefore, carbon nanomaterials combined with electrochemical methods can be used to detect miRNA quickly, easily and sensitively. In this review, we systematically review recent applications of different carbon nanomaterials (carbon nanotubes, graphene and its derivatives, graphitic carbon nitride, carbon dots, graphene quantum dots and other carbon nanomaterials) for miRNA electrochemical detection. In addition, we demonstrate the future prospects of electrochemical biosensors modified by carbon nanomaterials for the detection of miRNAs, and some suggestions for their development in the near future.

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Section: Carbon Nanomaterials For Microrna Electrochemical Biosensingmentioning

confidence: 99%

Recent Applications of Carbon Nanomaterials for microRNA Electrochemical Sensing

Wang

Wen

Yan

2020

Chemistry An Asian Journal

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“…Inorganic semiconductors such as titanium dioxide (TiO 2 ) (Tian et al, 2018), cadmium telluride (CdTe) (Lin et al, 2016; Hao et al, 2017b; Li et al, 2018a), cadmium sulfide (CdS) (Gong et al, 2015; Ge et al, 2016a; Lv et al, 2017), Molybdenum disulphide (MoS 2 ) (Zang et al, 2016; Wang et al, 2019), cadmium selenide (CdSe) (Matylitsky et al, 2009; Wang et al, 2014c), and zinc oxide (ZnO) (Han et al, 2017b) are used extensively in affinity based PEC biosensors (Hong et al, 2015; Lin et al, 2016; Hao et al, 2017b; Li et al, 2018a). Inorganic semiconductors offer a few advantages over their organic counterparts as discussed in the following section.…”

Section: Photoactive Species For Pec Biosensorsmentioning

confidence: 99%

“…These electron-hole pairs then take part in chemical reaction with the redox species in the electrolyte or interact with the underlying substrate to either generate a measurable signal or enhance an existing one (Figure 3a). Different types of photoactive species, such as semiconductor nanocrystals (Yue et al, 2013), metallic nanoparticles (Wang et al, 2019) and organic semiconductors such as g-C 3 N 4 , PFP (poly(9,9-bis(6′-(N,N,N,-trimethylammonium)hexyl) fluorene-co-alt-1,4-phenylene) bromide, etc. (Dai et al, 2016; Liu et al, 2018) have been commonly used as a signal label.…”

Section: Transduction Mechanismmentioning

confidence: 99%

Affinity-Based Detection of Biomolecules Using Photo-Electrochemical Readout

et al. 2019

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Detection and quantification of biologically-relevant analytes using handheld platforms are important for point-of-care diagnostics, real-time health monitoring, and treatment monitoring. Among the various signal transduction methods used in portable biosensors, photoelectrochemcial (PEC) readout has emerged as a promising approach due to its low limit-of-detection and high sensitivity. For this readout method to be applicable to analyzing native samples, performance requirements beyond sensitivity such as specificity, stability, and ease of operation are critical. These performance requirements are governed by the properties of the photoactive materials and signal transduction mechanisms that are used in PEC biosensing. In this review, we categorize PEC biosensors into five areas based on their signal transduction strategy: (a) introduction of photoactive species, (b) generation of electron/hole donors, (c) use of steric hinderance, (d) in situ induction of light, and (e) resonance energy transfer. We discuss the combination of strengths and weaknesses that these signal transduction systems and their material building blocks offer by reviewing the recent progress in this area. Developing the appropriate PEC biosensor starts with defining the application case followed by choosing the materials and signal transduction strategies that meet the application-based specifications.

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“…The electrochemical method represented three times higher sensitivity (0.71 pg·mL −1 ) than that of the plasmonic method (0.77 ng·mL −1 ), due to the combination of the three components to enhance the bio-affinity, biocompatibility, and electrochemical activity of the aptasensor. Recently, a photoelectrochemical biosensor was reported for miRNA-396a detection while using MoS 2 /g-C 3 N 4 /black TiO 2 as the platform and Histostar@AuNPs as the signal amplifier [196]. MoS 2 /g-C 3 N 4 /black TiO 2 and AuNPs were sequentially modified on ITO substrate to receive probe DNAs.…”

Section: Electrochemical Biosensors/sensors Based On the Other 2d mentioning

confidence: 99%

2D Materials in Development of Electrochemical Point-of-Care Cancer Screening Devices

et al. 2019

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Effective cancer treatment requires early detection and monitoring the development progress in a simple and affordable manner. Point-of care (POC) screening can provide a portable and inexpensive tool for the end-users to conveniently operate test and screen their health conditions without the necessity of special skills. Electrochemical methods hold great potential for clinical analysis of variety of chemicals and substances as well as cancer biomarkers due to their low cost, high sensitivity, multiplex detection ability, and miniaturization aptitude. Advances in two-dimensional (2D) material-based electrochemical biosensors/sensors are accelerating the performance of conventional devices toward more practical approaches. Here, recent trends in the development of 2D material-based electrochemical biosensors/sensors, as the next generation of POC cancer screening tools, are summarized. Three cancer biomarker categories, including proteins, nucleic acids, and some small molecules, will be considered. Various 2D materials will be introduced and their biomedical applications and electrochemical properties will be given. The role of 2D materials in improving the performance of electrochemical sensing mechanisms as well as the pros and cons of current sensors as the prospective devices for POC screening will be emphasized. Finally, the future scopes of implementing 2D materials in electrochemical POC cancer diagnostics for the clinical translation will be discussed.

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Photoelectrochemical biosensor for microRNA detection based on a MoS2/g-C3N4/black TiO2 heterojunction with Histostar@AuNPs for signal amplification

Cited by 112 publications

References 48 publications

Recent Applications of Carbon Nanomaterials for microRNA Electrochemical Sensing

Recent Applications of Carbon Nanomaterials for microRNA Electrochemical Sensing

Affinity-Based Detection of Biomolecules Using Photo-Electrochemical Readout

2D Materials in Development of Electrochemical Point-of-Care Cancer Screening Devices

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