Photoelectrochemical imaging system with high spatiotemporal resolution for visualizing dynamic cellular responses

Zhou, Bo; Das, Anirban; Zhong, Muchun; Guo, Qian; Zhang, De-Wen; Hing, Karin A.; Sobrido, Ana Jorge; Titirici, Maria‐Magdalena; Krause, Steffi

doi:10.1016/j.bios.2021.113121

Cited by 29 publications

(36 citation statements)

References 56 publications

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“…Among permeabilization methods there are protective and fluidifying agents. Organic solvents (dimethyl sulfoxide, dimethylformamide) induce mitochondrial swelling, enhance the permeation to H + /K + , collapse the potential of the inner mitochondrial membrane and increase its fluidity [ 69 ].…”

Section: Methods Applied For Permeabilizationmentioning

confidence: 99%

Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes

Bánfalvi

2022

IJMS

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Eukaryotic chromosomes have not been visualized during the interphase. The fact that chromosomes cannot be seen during the interphase of the cell cycle does not mean that there are no means to make them visible. This work provides visual evidence that reversible permeabilization of the cell membrane followed by the regeneration of cell membranes allows getting a glimpse behind the nuclear curtain. Reversibly permeable eukaryotic cells have been used to synthesize nascent DNA, analyze the 5′-end of RNA primers, view individual replicons and visualize interphase chromosomes. Dextran T-150 in a slightly hypotonic permeabilizing buffer prevented cells from disruption. Upon reversal of permeabilization, the nucleus could be opened at any time during the interphase. A broad spectrum of a flexible chromatin folding pattern was revealed through a series of transient geometric forms of chromosomes. Linear attachment of chromosomes was visualized in several mammalian and lower eukaryotic cells. The linear connection of chromosomes is maintained throughout the cell cycle showing that rather than individual chromosomes, a linear array of chromosomes is the functional giant macromolecule. This study proves that not only the prokaryotic genome but also linearly attached eukaryotic chromosomes form a giant macromolecular unit.

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Section: Methods Applied For Permeabilizationmentioning

confidence: 99%

Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes

Bánfalvi

2022

IJMS

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“…The LAE is a promising system for the imaging of cellular morphology and activity. The Krause group recently reported a photoelectrochemical imaging system to monitor cell lysis with a surfactant (Figure 5b) [87,88]. [85] with permission from IOP journals, copyright 2017.…”

Section: Lae As a Photoelectrochemical Imaging Sensormentioning

confidence: 99%

Light in Electrochemistry

2021

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Electrochemistry represents an important analytical technique used to acquire and assess chemical information in detail, which can aid fundamental investigations in various fields, such as biological studies. For example, electrochemistry can be used as simple and cost-effective means for bio-marker tracing in applications, such as health monitoring and food security screening. In combination with light, powerful spatially-resolved applications in both the investigation and manipulation of biochemical reactions begin to unfold. In this article, we focus primarily on light-addressable electrochemistry based on semiconductor materials and light-readable electrochemistry enabled by electrochemiluminescence (ECL). In addition, the emergence of multiplexed and imaging applications will also be introduced.

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“…CMOS based amperometric [89] SiNWFET PNA/DNA N/A 10 fm Zhang et al [126] SiNW DNA/DNA 2.5 × 10 13 molecules cm −2 10 pm Bunimovich. [127] Impedimetric DNA/DNA N/A 500 pm Wei et al [128] Amperometric COOH/LPS N/A 1.8 ng mL −1 Reta et al [129] LAE DNA/DNA N/A 500 pm Zarei et al [130] LAPS N/A N/A N/A Zhou et al [131] Figure 8. a) Illustration of a Si Nanowires on SiO 2 chip and b) Schematic Illustration of PNA functionalization that selectively bind to Si over enclosed SiO 2 .…”

Section: Electrochemical Sensorsmentioning

confidence: 99%

“…Zou et al. [ 131 ] further showed that functionalized oxide free silicon substrates may be a suitable for LAPS imaging. The authors presented an innovative photo‐electrochemical imaging sensing platform that combines the ability of LAPS to analyze dynamic ion fluxes with LAE that may give information about dynamic changes in cell attachment and permeability of the cell membrane.…”

Section: Recent Application Of Oxide Free Silicon‐based Biosensorsmentioning

confidence: 99%

Functionalization of Oxide‐Free Silicon Surfaces for Biosensing Applications

Henriksson

Neubauer

Birkholz

2021

Adv Materials Inter

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As an alternative to standard silicon biofunctionalization protocol based on alkoxysilanes that bind to SiO2 on oxidized silicon substrates, several protocols to form bifunctional interlayers that directly bind to the silicon surface via a strong SiC bond have been developed during the last decades. These interlayers are more stable, and the lack of an insulating layer between the substrate and the interlayer reduces electric noise in biosensor devices. SiC monolayers are not yet regularly applied as the protocols are more complex and generally require an inert gas atmosphere. However, a variety of applications and new methods to form bifunctional SiC interlayers are recently developed. Here, the role these innovative protocols and interlayers play in biofunctionalization of silicon surfaces is reviewed and their applications in electrochemical, microelectromechanical, and optical biosensing are reported. From the analysis of the current situation, it can be concluded that the advantageous properties offered with this approach in many cases more than outweigh the additional processes to form SiC bonded interlayers and the approach is predicted to expand the applications of future silicon‐based biosensors.

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Photoelectrochemical imaging system with high spatiotemporal resolution for visualizing dynamic cellular responses

Cited by 29 publications

References 56 publications

Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes

Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes

Light in Electrochemistry

Functionalization of Oxide‐Free Silicon Surfaces for Biosensing Applications

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