Photoinduced Prevention of Tissue Adhesion

Matsuda, Takehisa; Moghaddam, Minoo J.; Mlwa, H.; Sakurai, Katsukiyo; Iida, Futoshi

doi:10.1097/00002480-199207000-00008

Cited by 47 publications

(37 citation statements)

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“…For example, polyvinyl alcohol (Amanda and Mallapragada, 2001), polyacrylamide (Park et al, 2000), hyaluronic acid (Matsuda et al, 1992), dextran (Holland et al, 1998), and especially polyethylene glycol (PEG) (Scott and Murad, 1998;Zalipsky and Lee, 1992) have been used as coating materials for such purposes. The methods of surface treatment depend strongly upon the nature of the polymer being utilized and include covalent grafting of polymers (Desai and Hubbell, 1991), plasma treatment (Shen et al, 2001), physisorption (Holland et al, 1998;Neff et al, 1999), chemisorption (Winblade et al, 2000), incorporation of interpenetrating networks (Drumheller and Hubbell, 1995), and thin film deposition (Nikolova and Jones, 1998).…”

Section: Introductionmentioning

confidence: 99%

RGD‐grafted poly‐l‐lysine‐graft‐(polyethylene glycol) copolymers block non‐specific protein adsorption while promoting cell adhesion

VandeVondele

Vörös

Hubbell

2003

Biotech & Bioengineering

308

261

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A novel class of surface-active copolymers is described, designed to protect surfaces from nonspecific protein adsorption while still inducing specific cell attachment and spreading. A graft copolymer was synthesized, containing poly-(L-lysine) (PLL) as the backbone and substrate binding and poly(ethylene glycol) (PEG) as protein adsorption-resistant pendant side chains. A fraction of the grafted PEG was pendantly functionalized by covalent conjugation to the peptide motif RGD to induce cell binding. The graft copolymer spontaneously adsorbs from dilute aqueous solution onto negatively charged surfaces. The performance of RGD-modified PLL-g-PEG copolymers was analyzed in protein adsorption and cell culture assays. These coatings efficiently blocked the adsorption of serum proteins to Nb(2)O(5) and tissue culture polystyrene while specifically supporting attachment and spreading of human dermal fibroblasts. This surface functionalization technology is expected to be valuable in both the biomaterial and biosensor fields, because different signals can easily be combined, and sterilization and application are straightforward and cost-effective.

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Section: Introductionmentioning

confidence: 99%

RGD‐grafted poly‐l‐lysine‐graft‐(polyethylene glycol) copolymers block non‐specific protein adsorption while promoting cell adhesion

VandeVondele

Vörös

Hubbell

2003

Biotech & Bioengineering

308

261

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“…[5][6][7][8] A previous study demonstrated an ultraviolet-(UV) light-curable glue in which a viscous buffer solution of poly(ethylene glycol) diacrylate (PEGDA) in the presence of a UV-induced radical generator multiply coupled to the gelatin molecules, was converted to a water-swellable gel upon UV irradiation. 7,8 This enables rapid gelation, hemostasis, and wound healing.…”

Section: Introductionmentioning

confidence: 99%

Novel visible‐light‐induced photocurable tissue adhesive composed of multiply styrene‐derivatized gelatin and poly(ethylene glycol) diacrylate

Sajiki

Nakayama

et al. 2003

J Biomed Mater Res

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A novel photocurable tissue adhesive glue, which is composed of styrene-derivatized (styrenated) gelatin, poly(ethylene glycol) diacrylate (PEGDA), and carboxylated camphorquinone in phosphate-buffered saline (PBS), was prepared. The prototype formulation suitable for arterial repair was determined based on the gel yield, degree of swelling, tissue adhesive strength, and breaking (or burst) strength in vitro. The formulated photocurable tissue adhesive glue with an appropriate viscosity was converted to a water-swollen gel within 1 min of visible light irradiation. The tissue adhesive glue, which was coated on a rat abdominal aorta incised with a pair of scissors, was immediately converted to a swollen gel upon subsequent irradiation with visible light, and concomitantly hemostasis was completed. Histological examination showed that the produced gel was tightly adhered to the artery shortly after photoirradiation. The gel gradually degraded with time and was completely absorbed within 4 weeks after treatment. These results indicate that the photocurable glue developed here may serve as a tissue adhesive glue applicable to vascular surgery.

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“…Treatments in which a barrier is placed onto the surface of the damaged tissue have been explored, including oxidized regenerated cellulose, 2 expanded polytetrafluorethylene, 3 and chemically modified hyaluronic acid. 4,5 Other approaches that have been explored in animal models include barriers formed from photopolymerized networks based on polyethylene glycol (PEG), 6 gels from polymeric surfactants, 7 and lavages from viscous polymeric compounds, including hyaluronic acid, 8 high-molecular-weight dextran, 9 other polysaccharides, 10 and polyethylene glycol 4000. 11,12 Pharmaceutical treatments have also been studied, including nonsteroidal anti-inflamatory drugs, 13 anticoagulants, 14 fibrinolytic and fibrinogenolytic agents, [15][16][17] agents that promote intestinal movement, 18 mast cell stabilizers, 19 and oxygen free-radical scavengers.…”

Section: Introductionmentioning

confidence: 99%

Reduction of fibrous adhesion formation by a copolymer possessing an affinity for anionic surfaces

Elbert

Hubbell

1998

J. Biomed. Mater. Res.

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Postsurgical adhesions represent a common complication following a variety of surgical procedures. We sought to develop and evaluate a water-soluble polymer that could self-assemble onto tissue surfaces, forming a barrier on the surface. A copolymer was synthesized so as to contain two components: one component adsorbed to the tissue surface, and the other created a steric barrier, thereby preventing cell interactions with the tissue surface, and perhaps altering the wound-healing response that leads to the formation of fibrous adhesions. The component selected for tissue binding was a water-soluble polycation, poly-L-lysine, which can bind to negative sites on glycoproteins, proteoglycans, and cells; and the component selected for steric stabilization was polyethylene glycol, a nonionic polymer that interacts poorly with proteins. Efficacy of lavage with an aqueous solution of the copolymer for the prevention of postsurgical abdominopelvic adhesions was assessed following a standard electrocautery injury of the uterine horns of rats. The copolymer resulted in an 88% reduction in the extent of adhesions that formed. In vitro studies designed to investigate the mechanism of this efficacy indicated that the copolymer may both hinder cell-tissue adhesive interactions and alter the process of fibrin formation.

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Photoinduced Prevention of Tissue Adhesion

Cited by 47 publications

References 0 publications

RGD‐grafted poly‐l‐lysine‐graft‐(polyethylene glycol) copolymers block non‐specific protein adsorption while promoting cell adhesion

RGD‐grafted poly‐l‐lysine‐graft‐(polyethylene glycol) copolymers block non‐specific protein adsorption while promoting cell adhesion

Novel visible‐light‐induced photocurable tissue adhesive composed of multiply styrene‐derivatized gelatin and poly(ethylene glycol) diacrylate

Reduction of fibrous adhesion formation by a copolymer possessing an affinity for anionic surfaces

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