Photoluminescence Activation of Organic Dyes via Optically Trapped Quantum Dots

Abstract

Laser tweezers afford quantum dot (QD) manipulation for its use as localized emitters.Here, we demonstrate fluorescence by radiative energy transfer from optically trapped colloidal QDs (donors) to fluorescent dyes (acceptors). To this end, we synthesized silica-coated QDs of different compositions and triggered their luminescence by simultaneous trapping and two-photon excitation in a microfluidic chamber filled with dyes. This strategy produces a near-field light source with great spatial maneuverability, which can be exploited to scan nanostructures. In this regard, we demonstrate induced photo-luminescence of dye-labelled cells via optically trapped silica-coated colloidal QDs placed at their vicinity. Allocating nanoscale donors at controlled distances from a

Graphene Oxide Based Two-Dimensional Optical Tweezers for Low Power Trapping of Quantum Dots and E. coli Bacteria

“…30 There have been only a few reports on optical trapping of colloidal QDs. 3,13,31,32 Oddershede and co-workers had reported three-dimensional optical control using infrared lasers. 3 The power used for trapping the QDs was reported to be 100 mW at the sample (0.525 W at laser output).…”

“…However, it is known that trapping such small nano-sized particles is challenging as it requires high intensities to overcome the thermal Brownian motion . There have been only a few reports on optical trapping of colloidal QDs. ,,, Oddershede and co-workers had reported three-dimensional optical control using infrared lasers . The power used for trapping the QDs was reported to be 100 mW at the sample (0.525 W at laser output).…”

Magnetic Field-Dependent Photoluminescence of Tartrate-Functionalized Gadolinium-Doped Manganese Ferrite Nanoparticles: A Potential Therapeutic Agent for Hyperbilirubinemia Treatment

“…The past decade of modern technology has perceived a surge in interest for developing sensitive analytical techniques in the areas of biological science, pharmacy, medical, and cellular biology. 1−9 Photoluminescence (PL) is one of the prior techniques for bio-analytical purposes and is closely related to the quantized energy states of fluorescent materials, for example, quantum dots, 10 organic dyes, 11,12 and proteins. 13−15 The light emission in the ultraviolet and visible regions of these fluorophores has till now facilitated numerous clinical diagnosis such as fluorescence imaging, 16−20 cell labeling and tracking, 21−25 and biosensing.…”

“…The past decade of modern technology has perceived a surge in interest for developing sensitive analytical techniques in the areas of biological science, pharmacy, medical, and cellular biology. − Photoluminescence (PL) is one of the prior techniques for bio-analytical purposes and is closely related to the quantized energy states of fluorescent materials, for example, quantum dots, organic dyes, , and proteins. − The light emission in the ultraviolet and visible regions of these fluorophores has till now facilitated numerous clinical diagnosis such as fluorescence imaging, − cell labeling and tracking, − and biosensing. − Among various fluorophores, magneto-fluorescent nanomaterials have gained enormous research attention because they can intensify the PL intensity by targeting a specific site with the help of an external magnetic field (B). In addition, the size tunability of magneto-fluorescent nanomaterials from a few nanometers to tens of nanometers allows matching with various biomolecules, which can significantly enhance their interaction capability with different biological entities. − Hence, excellent target selectivity and high interaction probability of magneto-fluorescent nanomaterials can provide a high-quality fluorescence image with good bright/dark contrast, which is beneficial to diversified biomedical applications. − …”

Fluorescence Imaging as a Tool in Preclinical Evaluation of Polymer-Based Nano-DDS Systems Intended for Cancer Treatment

“…Nevertheless, the fluorescent probes and methods used for their application can greatly affect various processes, including the ligand specificity, intracellular targeting efficacy, concentration ranges for target detection, optimal resolution, and sensitivity. The increasing availability of fluorescent dyes [33,34,35], smart fluorophores [36,37], quantum dots [34,38], metallic nanoparticles [39], composite nanoshells [36], or fluorescent proteins [40,41,42] has facilitated the development and applicability of FI in recent years. From the polymer-based DDS point of view the best candidates are the fluorescent dyes, as they are small in size with respect to the polymer DDS systems.…”