Photon Emission by Heme-Protein in the Presence of Reactive Oxygen Species and Phenolic Compounds

Yoshiki, Yumiko; Kawane, M.; Iida, Tetsuo; Yuan, Huiqing; Okubo,; Ishizawa, Takenori; Kawabata, Sueo

doi:10.1021/bk-1998-0702.ch025

Cited by 7 publications

(3 citation statements)

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“…Yoshiki et al (5,6) reported that a low-level chemiluminescence arising from the H 2 O 2 -flavonoid-acetaldehyde system could be observed by mixing three species-reactive oxygen species (X), hydrogen donor (Y) and mediator (Z)-at room temperature. In addition, the photon intensity (P) in this system shows a high concentration dependency on the X, Y and Z species, which leads to the following equation:…”

Section: Introductionmentioning

confidence: 99%

Screening of chemiluminescence constituents of cereals and DPPH radical scavenging activity of γ‐oryzanol

et al. 2001

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The chemiluminescence (CL) constituents of cereals were detected by CL using the H(2)O(2)-acetaldehyde system. The cereals tested, such as rice, millet and sorghum, exhibited various levels of CL activity. The gamma-oryzanol fraction was extracted from brown rice and separated into four constituents by HPLC. The four constituents were identified as cycloartenyl ferulate, 24-methylenecycroartanyl ferulate, campesteryl ferulate and beta-sitosteryl ferulate. Free radical scavenging activities with 1,1-diphenyl-2-picrylhydrazyl (DPPH) and CL intensities of four constituents (gamma-oryzanol components) were measured and compared with that of gallic acid, which is a typical free radical scavenger. Four constituents scavenged DPPH radicals and scavenging activities were proportional to CL intensities. Concentrations of four CL constituents required to quench 50% (IC(50)) of the free radicals ranged from 0.9 to 1.1 mmol/L. We demonstrated that measurement of CL intensities was a rapid and convenient method for screening DPPH radical scavenging activities of rice.

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Section: Introductionmentioning

confidence: 99%

Screening of chemiluminescence constituents of cereals and DPPH radical scavenging activity of γ‐oryzanol

et al. 2001

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“…Yoshiki et al 11) have reported for a XYZ photon emission system that the presence of a catalyst such as an aldehyde compound and heme compound improved the reaction of ROS with a hydrogen donor through electron translation. They have further demonstrated that understanding the photon emission mechanism was beneˆcial studies on the antioxidative mechanism, since the photon intensity and radical-scavenging activity were closely correlated in the presence of these catalytic compounds.…”

Section: Resultsmentioning

confidence: 99%

Generation of Reactive Oxygen Species and Photon Emission from a Browned Product

Iida

Yoshiki

Someya

et al. 2002

Bioscience, Biotechnology, and Biochemistry

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The properties of photon emission arising from a browned product were investigated. The photon intensity of the browned product was proportional to the absorbancy at 420 nm, and was influenced by the amino acid structure. The fluorescence spectrum showed similar compounds in the browned product to be related with this photon emission. Superoxide and hydrogen peroxide contributed highly to this photon emission, and several redox compounds enhanced the photon intensity at appropriate concentrations. Our work suggests that the photon intensity was closely related to the reactive oxygen species (ROS) generated from the browned product, and this effect may be utilized to evaluate the function and quality of browned food.

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“…However, our previous study demonstrated a low-level chemiluminescence from the H 2 O 2 /gallic acid/hemoprotein, suggesting that this chemiluminescence arose from excited triplet carbonyls through the peroxidase-like activity of hemoprotein (11)(12)(13). The combination of gallic acid and hemoprotein, which are chemiluminescence agents in this system, showed synergistic activity to the hydroxyl radical and O 2 Ϫ inhibitions and antioxidative effect (11)(12)(13). Therefore, we hypothesized the H 2 O 2 -scavenging pathway to explain the mechanism of the hemoprotein chemiluminescence.…”

Section: Introductionmentioning

confidence: 80%

Chemiluminescence of Hemoglobin and Identification of Related Compounds with the Hemoglobin Chemiluminescence in Plasma¶

Yoshiki¹,

Iida²,

Okubo³

et al. 2007

Photochemistry and Photobiology

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A low level of chemilumnescence by hemoglobin (Hb) was detected in the reaction with H2O2 and hydrogen donors such as gallic acid and catechins. The photon intensity was affected by the ferric state of Hb (methemoglobin > oxyhemoglobin), and was roughly correlated with the radical‐scavenging potential of catechins. We hypothesized the reversible activation reaction of Hb as the chemiluminescence mechanism of the H2O2/gallic acid/Hb system. It is indicated that the oxidized‐Hb (Hb‐OOH) formation was a chemiluminescence‐rate–determining step and one‐electron reduction by a hydrogen donor of the compound‐I–type intermediate ([·XFeIV]= O) proved a chemiluminescence‐specificity–determining step. Spectral analysis showed that the photon emission from the H2O2/gallic acid/Hb system was produced without singlet oxygen generation. The concentration dependence of photon intensity suggests a high consumption ratio of H2O2 leading to protection from H2O2 toxicity. Albumin was defined as a hydrogen donor by the isolation of chemiluminescent substance in plasma using this chemiluminescence system.

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Photon Emission by Heme-Protein in the Presence of Reactive Oxygen Species and Phenolic Compounds

Cited by 7 publications

References 0 publications

Screening of chemiluminescence constituents of cereals and DPPH radical scavenging activity of γ‐oryzanol

Screening of chemiluminescence constituents of cereals and DPPH radical scavenging activity of γ‐oryzanol

Generation of Reactive Oxygen Species and Photon Emission from a Browned Product

Chemiluminescence of Hemoglobin and Identification of Related Compounds with the Hemoglobin Chemiluminescence in Plasma¶

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