Photoperiodic influence on the innervation of the ductus epididymidis and ductus deferens of the Djungarian hamster, Phodopus sungorus: electron-microscopic and biochemical results

Schindelmeiser, Jochen; Kutzner, M; Rolf, L. H.; Hoffmann, K.

doi:10.1007/bf00224732

Cited by 7 publications

(2 citation statements)

References 17 publications

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“…In camel epididymis NPY-positive fibres were reported to co-localize with dopamine beta hydroxylase (DBH). A colocalization of NPY with DBH has also been described in the man (Tainio et al, 1994), hamster, (Schindelmeiser et al, 1989), bull, (Rose et al, 1992), cat (Gürtler, 2001) and pig (Kaleczyc et al, 1997(Kaleczyc et al, , 1999. For this reason NPY seems to be a modulator of adrenergic neurotransmission, playing a role in the ejaculation and sperm transport (LamanoCarvalho et al, 1986;Mirabella et al, 2003;) and regulation of blood flow (Tainio et al, 1994).…”

Section: Resultsmentioning

confidence: 93%

Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

Liguori

Paino

Squillacioti

et al. 2013

Italian Journal of Animal Science

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Alpacas (Vicugna pacos) are domesticated camelids indigenous to south America and recently also bred in Europe and Italy for their high quality wool. There is little data available regarding the innervation of the male reproductive tract of this species. In the present study, the distribution of protein gene product 9.5 (PGP 9.5), neuropeptide Y (NPY), tyrosine hydroxilase (TH), calcitonin gene related peptide (CGRP) and substance P (SP) was analyzed in the epididymis by using immunohistochemical methods. Specimens of the caput, corpus and cauda epididymis were fixed in Bouin's fluid and processed for immunohistochemistry analysis with primary antibodies against PGP 9.5, NPY, TH, CGRP and SP. Immunopositivity to PGP 9.5 and TH and NPY was observed in nerve fibre bundles and in single nerve fibres contained into the peritubular connective tissue. Many TH and NPY immunopositive cells were found to innervate blood vessels. Rare CGRP and SP immunopositive nerves were observed. Several PGP 9,5 and NPY immunopositive epithelial cells were observed in the caput epididymis. The results of the present study suggest a role for the innervations in modulate reproductive functions in the alpaca epididymis.

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Section: Resultsmentioning

confidence: 93%

Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

Liguori

Paino

Squillacioti

et al. 2013

Italian Journal of Animal Science

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“…An increasing density of innervation along the smooth muscle layer of the whole sperm-transporting duct was demonstrated for adrenergic nerves of andrologia 21, No. 6 (1989) the rat (Baumgarten et al -1971) and for adrenergic (Schindelmeiser et al -1989 a) and NPY containing fibres (this study) of Phodopus sungorus, too.…”

Section: Discussionmentioning

confidence: 52%

Immunohistochemical Localization of Neuropeptide Y in Nerves of the Male Genital Tract of the Photoperiodically Responsive Djungarian Hamster, Phodopus Sungoras/Immunhistochemische Lokalisation von Neuropeptid Y in Nerven der männlichen Genitalorgane des

2009

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Neuropeptide Y (NPY) was demonstrated by immunohistochemistry in nerves of the male genital tract of Phodopus sungorus at long (LD 16%) und short (LD 8:16) photoperiods. No immunoreactive nerve fibres could be demonstrated in the testis, caput and corpus epididymidis and the ventral prostate gland. Dense networks of NPY-containing nerve fibers were demonstrated in the smooth muscle layer of the sperm-transporting duct, beginning in the cauda epididymidis with increasing density towards the distal part of the ductus deferens, and in the smooth muscle layer of the seminal vesicles. At short photoperiods, the density of the NPY-containing nerve plexus decreased only in the smooth muscle layer of the ductus deferens. A "trophic" influence of the large smooth muscle cells of the ductus deferens on their nerves not only in regard to their noradrenalin, but also on their NPY content is discussed.

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Postnatal development and distribution of peptide-containing nerves in the genital system of the male rat

1992

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The distribution and relative density of peptide-containing nerves was studied in the rat in order to assess the progression of neuronal changes during the postnatal development of the male genital system from the prepubertal age to adulthood. Testis, caput and cauda epididymis, ductus deferens, seminal vesicles, prostate and penis from 8-, 20-, 38-, and 70-day-old rats were sectioned and were immunostained with antisera to the neuropeptides calcitonin gene-related peptide (CGRP), vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY), and to a general neuronal marker, protein gene product 9.5 (PGP 9.5). The testicular parenchyma and caput epididymis did not show any immunoreactivity. Very scattered CGRP-containing nerves were present in 8-day-old rats; numerous VIP-, CGRP-, and NPY-peptide-containing nerves were observed in the cauda epididymis, ductus deferens, accessory glands and penis of 20-day-old rats. The number of nerves increased in 35-day-old rats while no changes were observed in more adult rats. A parallel increase was seen for the immunostain for PGP 9.5. These data suggest that peptide-containing nerves appear in the genital system after birth and reach a full development before the completion of puberty. Peptide-containing nerves were visible first in the interstitial area and then spread in the muscular coat of the ducts, glands and of the blood vessels. While CGRP- and NPY-containing nerves were distributed in the vicinity of the muscle cells, VIP-containing nerves were also observed in the subepithelial regions, suggesting a possible role of this neuropeptide in the control of epithelial functions.

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Photoperiodic influence on the innervation of the ductus epididymidis and ductus deferens of the Djungarian hamster, Phodopus sungorus: electron-microscopic and biochemical results

Cited by 7 publications

References 17 publications

Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

Immunohistochemical Localization of Neuropeptide Y in Nerves of the Male Genital Tract of the Photoperiodically Responsive Djungarian Hamster, Phodopus Sungoras/Immunhistochemische Lokalisation von Neuropeptid Y in Nerven der männlichen Genitalorgane des

Postnatal development and distribution of peptide-containing nerves in the genital system of the male rat

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