Photoprotection of Buddleja cordata extract against UVB-induced skin damage in SKH-1 hairless mice

Abstract

BackgroundIn recent years, there has been considerable interest in using botanical agents to prevent skin damage resulting from solar UV-irradiation. Buddleja cordata is a plant that is known as “tepozan”. Some people in Mexico use the leaves of this plant to treat tumours, abscesses, sores and burns. The purpose of this study is to investigate the photoprotective properties of Buddleja cordata methanolic extract (BCME) against UVB-induced skin damage in SKH-1 hairless mice at the macroscopic and histological levels.MethodsBCME was characterised to determine its spectroscopic, chromatographic and antioxidant (DPPH, superoxide and hydroxyl radicals) properties. To conduct the photoprotection studies, BCME was applied topically to the skin of SKH-1 mice before acute exposure to UVB for 10 minutes. The murine skin samples were used for macroscopic and histological studies to assess tissue damage. Penetration of active components of BCME into stratum corneum on the dorsal area of mice was investigated in vivo by the tape stripping method. Moreover, genotoxicity of BCME was evaluated in a Vicia faba cell root micronucleus model.ResultsBCME displayed absorbance over the entire UVB spectrum, and its principal components included verbascoside and linarin. BCME exhibited antioxidant activity and significantly scavenged hydroxyl radicals. BCME reduced erythema, sunburn cell production, vessel congestion and epidermal thickening of UVB irradiated mouse skin. BCME penetrate the skin of mice. BCME did not exhibit genotoxic activity in the micronucleus test.ConclusionThe topical administration of BCME protected against acute UVB-induced damage in mouse SKH-1 skin, and our results suggest that BCME may potentially prevent photodamage.

Reduction in ultraviolet B light–induced erythema by oxymetazoline and brimonidine is mediated by different α‐adrenoceptors

“…Repetitive UVB irradiation of hairless mice has been used previously to evaluate test compounds on skin photoageing, photodamage, inflammation and tumor development . UVB–induced erythema also has been used clinically in healthy volunteers to assess the photoprotective and anti‐inflammatory activity of potential therapeutic agents .…”

Fn14 deficiency protects lupus‐prone mice from histological lupus erythematosus‐like skin inflammation induced by ultraviolet light

“…Given the acute nature of the experimental protocol, features of both CLE and UVB damage were noted. Classic features found in UVB‐irradiated skin (24 hours following exposure) include thickening of the stratum corneum, epidermal spongiosis, microvesicles/pustules, sunburn cells and superficial vascular dilatation . While these features were seen in both Fn14WT and KO mice, the severity was significantly enhanced in WT.…”

In vivo ANTI-INFLAMMATORY ACTIVITY AND ACUTE TOXICITY OF METHANOLIC EXTRACTS FROM WILD PLANT LEAVES AND CELL SUSPENSION CULTURES OF Buddleja cordata Kunth (Buddlejaceae)

“…& Kunth, commonly known as Tepozán blanco, has been used diversely in Mexican traditional medicine as a treatment for several health disorders, such as tumors, abscesses, sores, burn wounds, rheumatic pains, skin illness and inflammation related to arthritis. Today, these ethno-medicinal uses require support from scientific studies (Ávila- Acevedo et al, 2014).…”