Photosynthesis, sugars and the regulation of gene expression

Pego, Jónatas V.; Kortstee, A.J.; Huijser, Casper; Smeekens, Sjef

doi:10.1093/jexbot/51.suppl_1.407

Cited by 246 publications

(159 citation statements)

References 88 publications

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“…The isolation and characterization of mutants has proved powerful in the dissection of numerous signal transduction pathways in Arabidopsis, such as phytochrome-mediated light perception (Smith, 2000); auxin, abscisic acid, and ethylene signaling (Leung and Giraudat, 1998; Callis and Vierstra, 2000; Stepanova and Ecker, 2000) and sugar sensing (Pego et al, 2000). In this work, we have exploited the fact that Chl fluorescence provides a non-destructive quantitative probe of changes in photosynthetic efficiency and/or capacity and have developed a mutant screen based on analysis and imaging of Chl fluorescence.…”

Section: Discussionmentioning

confidence: 99%

Identification of Mutants of Arabidopsis Defective in Acclimation of Photosynthesis to the Light Environment

Walters¹,

Shephard²,

Rogers³

et al. 2003

Plant Physiology

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In common with many other higher plant species, Arabidopsis undergoes photosynthetic acclimation, altering the composition of the photosynthetic apparatus in response to fluctuations in its growth environment. The changes in photosynthetic function that result from acclimation can be detected in a noninvasive manner by monitoring chlorophyll (Chl) fluorescence. This technique has been used to develop a screen that enables the rapid identification of plants defective atACCLIMATION OF PHOTOSYNTHESIS TO THE ENVIRONMENT(APE) loci. The application of this screen to a population of T-DNA-transformed Arabidopsis has successfully led to the identification of a number of mutant lines with altered Chl fluorescence characteristics. Analysis of photosynthesis and pigment composition in leaves from three such mutants showed that they had altered acclimation responses to the growth light environment, each having a distinct acclimation-defective phenotype, demonstrating that screening for mutants using Chl fluorescence is a viable strategy for the investigation of acclimation. Sequencing of the genomic DNA flanking the T-DNA elements showed that in the ape1mutant, a gene was disrupted that encodes a protein of unknown function but that appears to be specific to photosynthetic organisms, whereas the ape2 mutant carries an insertion in the region of the TPT gene encoding the chloroplast inner envelope triose phosphate/phosphate translocator.

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Section: Discussionmentioning

confidence: 99%

Identification of Mutants of Arabidopsis Defective in Acclimation of Photosynthesis to the Light Environment

Walters¹,

Shephard²,

Rogers³

et al. 2003

Plant Physiology

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show abstract

“…We notice that several genes with negative correlation to Suc encode photosynthesis-related proteins, which highlights the inhibitory effect of Suc on photosynthesis (Pego et al, 2000). Other negatively correlated genes are glycosyl hydrolases with b-glucosidase activities and glycosyl hydrolases that presumably act on Man and Xyl (Supplemental Table S7).…”

Section: Sugar Signaling Network May Cause Diurnal Regulation Of a Lmentioning

confidence: 93%

Significance of Light, Sugar, and Amino Acid Supply for Diurnal Gene Regulation in Developing Barley Caryopses

et al. 2010

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The caryopses of barley (Hordeum vulgare), as of all cereals, are complex sink organs optimized for starch accumulation and embryo development. While their early to late development has been studied in great detail, processes underlying the caryopses' diurnal adaptation to changes in light, temperature, and the fluctuations in phloem-supplied carbon and nitrogen have remained unknown. In an attempt to identify diurnally affected processes in developing caryopses at the early maturation phase, we monitored global changes of both gene expression and metabolite levels. We applied the 22 K Barley1 GeneChip microarray and identified 2,091 differentially expressed (DE) genes that were assigned to six major diurnal expression clusters. Principal component analysis and other global analyses demonstrated that the variability within the data set relates to genes involved in circadian regulation, storage compound accumulation, embryo development, response to abiotic stress, and photosynthesis. The correlation of amino acid and sugar profiles with expression trajectories led to the identification of several hundred potentially metabolite-regulated DE genes. A comparative analysis of our data set and publicly available microarray data disclosed suborgan-specific expression of almost all diurnal DE genes, with more than 350 genes specifically expressed in the pericarp, endosperm, or embryo tissues. Our data reveal a tight linkage between day/night cycles, changes in light, and the supply of carbon and nitrogen. We present a model that suggests several phases of diurnal gene expression in developing barley caryopses, summarized as starvation and priming, energy collection and carbon fixation, light protection and chaperone activity, storage and growth, and embryo development.

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“…Interestingly, these inhibitory effects are antagonized by nitrates, suggesting interplay between carbon and nitrogen status in the control of germination (Moore et al, 2003;Bi et al, 2005). Using screens for either resistance or sensitivity of germination to high sugar concentrations, sugar-insensitive or -hypersensitive mutants have been identified (Zhou et al, 1998;Laby et al, 2000;Pego et al, 2000;Rolland et al, 2002). Surprisingly, many sugar-insensitive mutants, such as sugar-insensitive4/glucose-insensitive1 (sis4/gin1) and sis5/gin6, are allelic to ABA synthesis (aba2) and ABA-insensitive (abi4) mutants, respectively (ArenasHuertero et al, 2000;Cheng et al, 2002).…”

mentioning

confidence: 99%

BT2, a BTB Protein, Mediates Multiple Responses to Nutrients, Stresses, and Hormones in Arabidopsis

et al. 2009

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The Arabidopsis (Arabidopsis thaliana) gene BT2 encodes a 41-kD protein that possesses an amino-terminal BTB domain, a central TAZ domain, and a carboxyl-terminal calmodulin-binding domain. We previously demonstrated that BT2 could activate telomerase expression in mature Arabidopsis leaves. Here, we report its distinct role in mediating diverse hormone, stress, and metabolic responses. We serendipitously discovered that steady-state expression of BT2 mRNA was regulated diurnally and controlled by the circadian clock, with maximum expression in the dark. This pattern of expression suggested that BT2 mRNA could be linked to the availability of photosynthate in the plant. Exogenous sugars decreased BT2 expression, whereas exogenous nitrogen increased expression. bt2 loss-of-function mutants displayed a hypersensitive response to both sugar-mediated inhibition of germination and abscisic acid (ABA)-mediated inhibition of germination, thus supporting a role of ABA in sugar signaling in germination and development. Moreover, constitutive expression of BT2 imparted resistance to both sugars and ABA at germination, suggesting that BT2 suppresses sugar and ABA responses. In support of the previously described antagonistic relationship between ABA and auxin, we found that BT2 positively regulated certain auxin responses in plants, as revealed by knocking down BT2 expression in the high-auxin mutant yucca. Accumulation of BT2 mRNA was affected by a variety of hormones, nutrients, and stresses, and BT2 was required for responses to many of these same factors. Together, these results suggest that BT2 is a central component of an interconnected signaling network that detects and responds to multiple inputs.

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Photosynthesis, sugars and the regulation of gene expression

Cited by 246 publications

References 88 publications

Identification of Mutants of Arabidopsis Defective in Acclimation of Photosynthesis to the Light Environment

Identification of Mutants of Arabidopsis Defective in Acclimation of Photosynthesis to the Light Environment

Significance of Light, Sugar, and Amino Acid Supply for Diurnal Gene Regulation in Developing Barley Caryopses

BT2, a BTB Protein, Mediates Multiple Responses to Nutrients, Stresses, and Hormones in Arabidopsis

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