Phototoxicity of argon laser irradiation on biofilms of Porphyromonas and Prevotella species

Henry, Clay A.; Dyer, Barbara; Wagner, Martin; Judy, Millard M.; Matthews, James L.

doi:10.1016/1011-1344(95)07239-x

Cited by 58 publications

(62 citation statements)

References 27 publications

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“…In exogenous PDT, we used TBO as photosensitizer, which was commonly used in periodontitis PDT studies. 6,9 We found out that di®erent kinds of bacteria responded di®erently to TBO-dependent PDT -P :m: was most PDT sensitive in our study. This was consistent with the previous¯nding that the e®ect of periodontitis PDT depended on bacterium species.…”

Section: Discussionmentioning

confidence: 56%

“…[9][10][11][12] In our study, using portable LED phototherapy devices, which are much smaller and less costly with easier access than the light sources in previous studies, we have demonstrated that both exogenous and endogenous PDT have killing e®ects in vitro on P :m:, P :g: and A:a: -the three main pathogenic bacteria in periodontitis. Meanwhile, BKEPs were screened out, which would be helpful to further preclinical or clinical studies.…”

Section: Discussionmentioning

confidence: 91%

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Toluidine blue O and porphyrin-mediated photodynamic therapy on three main pathogenic bacteria of periodontitis using portable LED phototherapy device

Jiang

Fan

et al. 2015

J. Innov. Opt. Health Sci.

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Photodynamic therapy (PDT) has been commonly used in treating many diseases, such as cancer and infectious diseases. We investigated the di®erent e®ects of PDT on three main pathogenic bacteria of periodontitis -Prevotella melaninogenica (P.m.), Porphyromonas gingivalis (P.g.) and Aggregatibacter actinomycetemcomitans (A.a.). The portable red light-emitting diode (LED) phototherapy device was used to assess the exogenous PDT e®ects with di®erent light doses and photosensitizer concentrations (Toluidine blue O, TBO). The portable blue LED phototherapy device was used to assess the endogenous PDT e®ects with the use of endogenous photosensitizers (porphyrin) under di®erent light doses. We found out that both exogenous and endogenous PDT were able to restrict the growth of all the three bacteria signi¯cantly. Moreover, the optimal PDT conditions for these bacteria were obtained through this in vitro screening and could guide the clinical PDT on periodontitis.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 91%

Toluidine blue O and porphyrin-mediated photodynamic therapy on three main pathogenic bacteria of periodontitis using portable LED phototherapy device

Jiang

Fan

et al. 2015

J. Innov. Opt. Health Sci.

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“…photodynamic treatment) has been reported in the literature (Wilson, 1994;Henry et al, 1995Henry et al, , 1996Konig et al, 2000;Soukos et al, 2005), and it has been suggested that this effect is mediated through the production of ROS (Gourmelon et al, 1994). This hypothesis is further supported by the observation that obligate anaerobic oral bacteria (pigmented and non-pigmented) are more susceptible to blue light exposure treatment than facultative ones (Feuerstein et al, 2004;Sterer & Feuerstein, 2005).…”

Section: Discussionmentioning

confidence: 97%

“…Various studies have demonstrated the antibacterial effect of visible light applied with or without exogenous photosensitizers (Wilson, 1994;Henry et al, 1995Henry et al, , 1996Konig et al, 2000;Soukos et al, 2005;Sterer & Feuerstein, 2005). It was further suggested that light-induced production of reactive oxygen species (ROS) is the mechanism underlying this phototoxic effect (Gourmelon et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Zinc enhances the phototoxic effect of blue light against malodour-producing bacteria in an experimental oral biofilm

Sterer

Jeffet

Dadoun

et al. 2014

Journal of Medical Microbiology

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Oral malodour is thought to be caused mainly by the production of volatile sulfide compounds (VSCs) by anaerobic Gram-negative oral bacteria. Previous studies have shown that these bacteria are susceptible to blue light (400-500 nm wavelength). In the present study, we tested the effect of blue light in the presence of zinc, erythrosine B or both on malodour production in an experimental oral biofilm. Biofilms were exposed to a plasma-arc light source for 30, 60 and 120 s (equal to energy fluxes of 41, 82 and 164 J cm "2 , respectively) with or without the addition of zinc acetate, erythrosine B or both. After the light exposure, biofilm samples were examined for malodour production (by an odour judge) and VSC production (with a Halimeter), and VSCproducing bacteria were quantified using a microscopy-based sulfide assay (MSA) and in situ confocal laser scanning microscopy (CLSM). Results showed that exposing experimental oral biofilm to both blue light and zinc reduced malodour production, which coincided with a reduction in VSC-producing bacteria in the biofilm. These results suggest that zinc enhances the phototoxicity of blue light against malodour-producing bacteria.

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“…The in vitro bactericidal effect of PDT on Gram-negative bacteria was also investigated and it was found to have a high bactericidal effect on Haemophilus parainfluenzae 34 , Prevotella and Porphyromonas species [35][36][37] , Helicobacter mustelae 38 , E. coli B, Acinetobacter baumannii, and P. aeruginosa 39,40 . PDT of Gram-positive and Gram-negative bacteria in animal models hasproven to be as successful as in in vitro studies in inactivating the causative bacteria [41][42][43][44][45][46] .…”

Section: Prophylactic Antimicrobials and Resistancementioning

confidence: 99%

Surgical site infections subjected to photodynamic therapy: a potential application in orthopaedic surgery

Jerjes¹,

Tan²,

Hopper³

et al. 2012

Hard Tissue

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Introduction Photodynamic therapy, a minimally invasive oncological modality, has been in use for over 20 years. However, little clinical data is available on its non-oncological efficacy. Several laboratory-based trials on animals have suggested that this technology may be applicable as an antimicrobial therapy. In orthopaedics, where implants and metal work are placed in deep tissue planes, a potential risk of infection is treated very seriously. Infection not only increases patient morbidity and mortality but also the burden on healthcare system. Proposing a new modality that can instantly tackle this problem. This critical review discusses surgical site infections subjected to photodynamic therapy. Conclusion Surgical care should be state of the art with careful attention to strategies that avoid the development of SSIs. When SSI does occur, superficial and deep wound infections can be treated using PDT by applying topical photosensitiser to the area followed by light illumination.

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Phototoxicity of argon laser irradiation on biofilms of Porphyromonas and Prevotella species

Cited by 58 publications

References 27 publications

Toluidine blue O and porphyrin-mediated photodynamic therapy on three main pathogenic bacteria of periodontitis using portable LED phototherapy device

Toluidine blue O and porphyrin-mediated photodynamic therapy on three main pathogenic bacteria of periodontitis using portable LED phototherapy device

Zinc enhances the phototoxic effect of blue light against malodour-producing bacteria in an experimental oral biofilm

Surgical site infections subjected to photodynamic therapy: a potential application in orthopaedic surgery

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