Phylogenetic analysis of Bombyx mori nucleopolyhedrovirus polyhedrin and p10 genes in wild isolates from Guangxi Zhuang Autonomous Region, China

Bombyx mori pylorus infection by Alphabaculovirus

“…In particular, the anterior area of the PIR was susceptible to the virus, and the first signs of infection were observed from the 5th dpi ( Figure 1A The anterior area of the PIR was found to be a secondary target of infection caused by the viral phenotype BV. This occurs because the cover provided by the intima, which is present on the luminal surface of the epithelium, represents a barrier to primary infection caused by ODVs (Ribeiro et al, 2009;Satadal et al, 2012;Liang et al, 2013). Moreover, the very action of digestive juice proteins, such as serine protease and fluorescent red, and the alkaline pH present in the midgut are responsible for the ODV release from the polyhedron, but can also degrade it, and they become inactivated if remaining in the lumen too long (Rohrmann, 2011;Sunagar et al, 2011;Qin et al, 2012;Liang et al, 2013).…”

“…The geometrical shape of BmMNPVs was described by Torquato et al (2006b) as a truncated octahedron. However, they may also present in other geometric forms, such as cuboids, hexahedral, dodecahedral, triangular, or tetrahedral, which may be the result of genetic variations specific to each viral strain, or even due to polyhedrin mutations (Katsuma et al, 1999;Hong et al, 2000;Ribeiro et al, 2009;Brancalhão et al, 2009;Liang et al, 2013).…”

“…This is surrounded by a membranous envelope and forms the enveloped nucleocapsid or virion. The several nucleocapsids of AlphaBV are grouped into an occlusion body protein or a polyhedron, which provides protection to the virions (Rohrmann, 2011;Liang et al, 2013;Ikeda et al, 2013).…”

“…According to the literature, MNPVs are more virulent than SNPVs, and there is evidence for geographical viral variability of NPVs to the same species, which may affect their virulence (Adams and McClintock, 1991;Hong et al, 2000;Fan et al, 2007;Liang et al, 2013). However, Hu et al (1999) and Rohrmann (2011) stated that the genetic basis for understanding the enveloping of one or more nucleocapsids remains unknown.…”

“…The knowledge gained from this study will contribute to a greater understanding of the infectious cycle of this important entomopathogenic virus, which is responsible for nuclear polyhedrosis disease, popularly known as grasserie or milky diseases. This is a serious worldwide problem for sericulture, for once the larvae are sick, they must be eliminated to prevent the spread and propagation of the disease in the environment (Sengupta et al, 1990;Qin et al, 2012;Satadal et al, 2012;Liang et al, 2013).…”