Phylogenetic analysis of bovine papillomavirus E5 detected in equine sarcoids in Poland

Abstract

The aim of the study was to analyse a part of the sequence of the E5 gene of bovine papillomaviruses (BPV) associated with equine sarcoids in Polish horses. Samples of 40 skin lesions obtained from 29 horses were collected for molecular examination. The PCR amplicons of BPV DNA were detected in 38 specimens. After phylogenetic analysis 37 specimens were recognized as BPV-1 and one as BPV-2. Phylogenetic analysis has allowed the classification of the amplicons into two phylogenetic groups (A1,) and four separate isolates (2, 10, 16, 17).

Quantitative PCR High-Resolution Melting (qPCR-HRM) curve analysis, a new approach to rapid detection and differentiation of bovine papillomavirus detected in equine sarcoids

“…The results were closely correlated with those previously described (Szczerba-Turek et al 2011). The point mutation in HRM analysis exists in 3763-4002 in comparison with BPV-1 (X02346) -described as a "wild".…”

“…The qPCR-HRM results were in high concordance with the sequencing results described previously (Szczerba-Turek et al 2011). HRM analysis showed that 6 (16%), 24 (63%) and 8 (21%) specimens were identified as BPV-1 -"wild", BPV-1 -"European" and BPV "variation", respectively (Table 1).…”

“…All of these results were confirmed in phylogenetic analysis of E5 ORF complete CDS (Table 2). Only four isolates: 3, 14b, 18, 24b were recognized not exactly the same as previously (Szczerba-Turek et al 2011). In the present study, they were recognized as a BPV "variation" of E5 ORF partial CDS, while previously, after phylogenetic analysis of E5 ORF partial CDS isolates, 3 and 18 were recognized as a "European" BPV-1 (phylogenetic group D) and isolates 14b and 24b were recognized as a "wild" BPV-1 (phylogenetic group A1) (Szczerba-Turek et al 2011).…”

“…Only four isolates: 3, 14b, 18, 24b were recognized not exactly the same as previously (Szczerba-Turek et al 2011). In the present study, they were recognized as a BPV "variation" of E5 ORF partial CDS, while previously, after phylogenetic analysis of E5 ORF partial CDS isolates, 3 and 18 were recognized as a "European" BPV-1 (phylogenetic group D) and isolates 14b and 24b were recognized as a "wild" BPV-1 (phylogenetic group A1) (Szczerba-Turek et al 2011). The phylogenetic analysis of these short nucleotide sequences (250 bp) was conducted for all specimens examined and the results of HRM analysis may suggest the first selection for future sequencing.…”

Equine Sarcoid

“…In Europe and Australia, BPV-1 is most detected. In eastern USA, an almost equal proportion of both virus types was found, whereas in Canada and Western USA, BPV-2 was demonstrated in most of the samples [12][13][14][15][16][17].…”