Phylogenetic analysis of the NS5 gene of dengue viruses isolated in Ecuador

Regato, Mary; Recarey, Ricardo; Moratorio, Gonzalo; Mora, Doménica de; García-Aguirre, Laura; González, Manuel; Mosquera, Carlos; Alava, Aracely; Fajardo, Álvaro; Alvarez, Macarena; Andrea, Lucía D’; Dubra, Ana; Martínez, Mariela; Khan, Baldip; Cristina, Juan

doi:10.1016/j.virusres.2007.10.012

Cited by 16 publications

(11 citation statements)

References 23 publications

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“…Although the Ecuadorian strains did not cluster with the Brazilian DENV-3 isolate (strain EF110568), recent studies suggest that DENV-3 might also have been introduced to Brazil from the Caribbean region [63]. …”

Section: Resultsmentioning

confidence: 99%

Dengue in Latin America: Systematic Review of Molecular Epidemiological Trends

et al. 2017

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Dengue, the predominant arthropod-borne viral disease affecting humans, is caused by one of four distinct serotypes (DENV-1, -2, -3 or -4). A literature analysis and review was undertaken to describe the molecular epidemiological trends in dengue disease and the knowledge generated in specific molecular topics in Latin America, including the Caribbean islands, from 2000 to 2013 in the context of regional trends in order to identify gaps in molecular epidemiological knowledge and future research needs. Searches of literature published between 1 January 2000 and 30 November 2013 were conducted using specific search strategies for each electronic database that was reviewed. A total of 396 relevant citations were identified, 57 of which fulfilled the inclusion criteria. All four dengue virus serotypes were present and co-circulated in many countries over the review period (with the predominance of individual serotypes varying by country and year). The number of countries in which more than one serotype circulated steadily increased during the period under review. Molecular epidemiology data were found for Argentina, Bolivia, Brazil, the Caribbean region, Colombia, Ecuador, Mexico and Central America, Paraguay, Peru and Venezuela. Distinct lineages with different dynamics were found in each country, with co-existence, extinction and replacement of lineages occurring over the review period. Despite some gaps in the literature limiting the possibility for comparison, our review has described the molecular epidemiological trends of dengue infection. However, several gaps in molecular epidemiological information across Latin America and the Caribbean were identified that provide avenues for future research; in particular, sequence determination of the dengue virus genome is important for more precise phylogenetic classification and correlation with clinical outcome and disease severity.

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Section: Resultsmentioning

confidence: 99%

Dengue in Latin America: Systematic Review of Molecular Epidemiological Trends

et al. 2017

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“…After an absence of 17 years from the Latin American region, DENV-3 re-emerged in Central America in 1994 [23], and continue to expand into South America [8,17,24-32]. Previous studies have shown that this emerging DENV-3 is a genotype III variant of Asiatic origin [8,24-26,33].…”

Section: Discussionmentioning

confidence: 99%

Evolution of Dengue Virus Type 3 Genotype III in Venezuela: Diversification, Rates and Population Dynamics

Ramírez

Fajardo

Moros

et al. 2010

Virol J

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BackgroundDengue virus (DENV) is a member of the genus Flavivirus of the family Flaviviridae. DENV are comprised of four distinct serotypes (DENV-1 through DENV-4) and each serotype can be divided in different genotypes. Currently, there is a dramatic emergence of DENV-3 genotype III in Latin America. Nevertheless, we still have an incomplete understanding of the evolutionary forces underlying the evolution of this genotype in this region of the world. In order to gain insight into the degree of genetic variability, rates and patterns of evolution of this genotype in Venezuela and the South American region, phylogenetic analysis, based on a large number (n = 119) of envelope gene sequences from DENV-3 genotype III strains isolated in Venezuela from 2001 to 2008, were performed.ResultsPhylogenetic analysis revealed an in situ evolution of DENV-3 genotype III following its introduction in the Latin American region, where three different genetic clusters (A to C) can be observed among the DENV-3 genotype III strains circulating in this region. Bayesian coalescent inference analyses revealed an evolutionary rate of 8.48 × 10-4 substitutions/site/year (s/s/y) for strains of cluster A, composed entirely of strains isolated in Venezuela. Amino acid substitution at position 329 of domain III of the E protein (A→V) was found in almost all E proteins from Cluster A strains.ConclusionsA significant evolutionary change between DENV-3 genotype III strains that circulated in the initial years of the introduction in the continent and strains isolated in the Latin American region in recent years was observed. The presence of DENV-3 genotype III strains belonging to different clusters was observed in Venezuela, revealing several introduction events into this country. The evolutionary rate found for Cluster A strains circulating in Venezuela is similar to the others previously established for this genotype in other regions of the world. This suggests a lack of correlation among DENV genotype III substitution rate and ecological pattern of virus spread.

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“…Four serotypes were circulating at that time and the circulation of DENV-2 and DENV-3 were later confirmed by the National Institute of Hygiene and Tropical Medicine of Ecuador. Few DHF cases were reported 6,117 (Figure 2A). Paraguay -outbreak 2000.…”

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confidence: 99%

The History of Dengue Outbreaks in the Americas

Dick

Martín²,

Montoya³

et al. 2012

The American Society of Tropical Medicine and Hygiene

368

264

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Dengue is a viral disease usually transmitted by Aedes aegypti mosquitoes. Dengue outbreaks in the Americas reported in medical literature and to the Pan American Health Organization are described. The outbreak history from 1600 to 2010 was categorized into four phases: Introduction of dengue in the Americas (1600–1946); Continental plan for the eradication of the Ae. aegypti (1947–1970) marked by a successful eradication of the mosquito in 18 continental countries by 1962; Ae. aegypti reinfestation (1971–1999) caused by the failure of the mosquito eradication program; Increased dispersion of Ae. aegypti and dengue virus circulation (2000–2010) characterized by a marked increase in the number of outbreaks. During 2010 > 1.7 million dengue cases were reported, with 50,235 severe cases and 1,185 deaths. A dramatic increase in the number of outbreaks has been reported in recent years. Urgent global action is needed to avoid further disease spread.

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Phylogenetic analysis of the NS5 gene of dengue viruses isolated in Ecuador

Cited by 16 publications

References 23 publications

Dengue in Latin America: Systematic Review of Molecular Epidemiological Trends

Dengue in Latin America: Systematic Review of Molecular Epidemiological Trends

Evolution of Dengue Virus Type 3 Genotype III in Venezuela: Diversification, Rates and Population Dynamics

The History of Dengue Outbreaks in the Americas

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