Phylogenetic Comparison of Endosymbionts with Their Host Ants Based on Molecular Evidence

Sameshima, Shinya; Hasegawa, Eisuke; Kitade, Osamu; Minaka, Nobuhiro; Matsumoto, Tadao

doi:10.2108/zsj.16.993

Cited by 49 publications

(64 citation statements)

References 25 publications

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“…In the future, 16S rDNA combined with the gyrB sequence method may be able to clarify molecular relationships of endosymbiotic Enterobacteriaceae (Heddi et al, 1998 ;Chen et al, 1999 ;Sameshima et al, 1999) and to identify viable but non-culturable Enterobacteriaceae in the environment (Vuddhakul et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae: a model molecule for molecular systematic studies.

Dauga¹

2002

International Journal of Systematic and Evolutionary Microbiology

174

112

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Phylogenetic trees showing the evolutionary relatedness ofEnterobacteriaceae based upon gyrB and 16S rRNA genes were compared. Congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. Phylogenetic trees based on gyrB sequences appeared to be more reliable at determining relationships among Serratia species than trees based on 16S rRNA gene sequences. gyrB sequences from Serratia species formed a monophyletic group validated by significant bootstrap values. Serratia fonticola had the most deeply branching gyrB sequence in the Serratia monophyletic group, which was consistent with its atypical phenotypic characteristics. Klebsiella and Enterobacter genera seemed to be polyphyletic, but the branching patterns of gyrB and 16S rRNA gene trees were not congruent. Enterobacter aerogenes was grouped with Klebsiella pneumoniae on the gyrB phylogenetic tree, which supports that this species could be transferred to the Klebsiella genus. Unfortunately, 16S rRNA and gyrB phylogenetic trees gave conflicting evolutionary relationships for Citrobacter freundii because of its unusual gyrB evolutionary process. gyrB lateral gene transfer was suspected for Hafnia alvei. Saturation of gyrB genes was observed by the pairwise comparison of Proteus spp., Providencia alcalifaciens and Morganella morganii sequences. Depending on their level of variability, 16S rRNA gene sequences were useful for describing phylogenetic relationships between distantly related Enterobacteriaceae, whereas gyrB sequence comparison was useful for inferring intra-and some intergeneric relationships.

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Section: Discussionmentioning

confidence: 99%

Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae: a model molecule for molecular systematic studies.

Dauga¹

2002

International Journal of Systematic and Evolutionary Microbiology

174

112

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“…In the tribe Camponotini, the omnivorous ants of the genus Camponotus have established an association with the intracellular bacteria Blochmannia and it has been a fundamental partner by improving colony growth and the host immune system (Souza et al, 2009). The congruence in the topology of bacteria and ant phylogeny suggests it has initiated in a common ancestral followed by the coevolution between the partners (Sameshima et al, 1999;Sauer et al, 2000). Moreover, genomic analysis of Blochmannia from host divergent lineages has showed differential loss of genes that affect cellular functions and metabolic pathways and this variation have been linked to distinct host-associated pressures (Williams and Wernegreen, 2015).…”

Section: Introductionmentioning

confidence: 99%

Bacterial Communities in the Midgut of Ponerine Ants (Hymenoptera: Formicidae: Ponerinae)

Oliveira¹,

Ferro²,

Bacci³

et al. 2016

Sociobiology

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Symbiotic microorganisms are directly related to the ecological success of host insects, influencing many aspects of their biology. The present study is the first to investigate the microbiota associated with ants of the subfamily Ponerinae and aims to identify the bacterial midgut communities of Dinoponera lucida, Neoponera curvinodis, Pachycondyla striata, Odontomachus brunneus and Odontomachus bauri relied on culture-dependent technique, particularly 16S rRNA sequencing. The greatest species richness was observed in O. bauri, with 15 OTUs, followed by D. lucida with five OTUs, O. brunneus, with four OTUs, and N. curvinodis and P. striata, both with three OTUs. There were representatives of the phyla Actinobacteria, Proteobacteria, Tenericutes and Firmicutes, including the genera Bartonella, Mesoplasma, Mesorhizobium, Spiroplasma, Wolbachia and Serratia in the guts of the studied Ponerine ants. Spiroplasma and Mesoplasma were found to be prevalent in the studied ants and they were the only genera of bacteria found in more than one of the analyzed ant species suggesting they might be beneficial symbionts. The low microbial diversity observed given the predatory trophic habits of the species studied suggests that there is selection for these microorganisms, predominantly preserving symbionts with functional roles that are able to colonize this environment. It is also valid to infer that the identified bacteria are predominant in the gut and exhibit mutualistic functions that are important mainly for immunity, but also to reproduction and nutrition; moreover, a subset may be parasites that could have considerable impacts on the studied ants.

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“…So far, Blochmannia spp. could be detected in every Camponotus species (Ͼ30) investigated, indicating an important role of the bacteria for the biology of the host animals (8,18,20). The bacterial endosymbionts reside in specialized cells, the bacteriocytes, which are intercalated between enterocytes of the midgut tissue of the host.…”

mentioning

confidence: 99%

Relevance of the Endosymbiosis of Blochmannia floridanus and Carpenter Ants at Different Stages of the Life Cycle of the Host

Zientz¹,

Beyaert

Gross³

et al. 2006

Appl Environ Microbiol

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Expression of several genes possibly involved in the symbiotic relationship between the obligate intracellular endosymbiont Blochmannia floridanus and its ant host Camponotus floridanus was investigated at different developmental stages of the host by real-time quantitative PCR. These included a set of genes related to nitrogen metabolism (ureC, ureF, glnA, and speB) as well as genes involved in the synthesis of the aromatic amino acid tyrosine (tyrA, aspC, and hisC). The overall transcriptional activity of Blochmannia was found to be quite low during early developmental stages and to increase steadily with host age. However, a concerted peak of gene expression related to nitrogen recycling could be detected around the entire process of pupation, while expression of biosynthesis pathways for aromatic amino acids was elevated only during a short phase in pupation. These data suggest an important role of certain metabolic functions for the symbiotic interactions of the bacteria and an individual host organism in early phases of development. General relevance of Blochmannia for its ant host was tested in fostering experiments with worker groups of Camponotus floridanus, and their success in raising pupae from first-instar larvae was used as a fitness measure. Groups treated with antibiotics had a significantly reduced success in raising the brood in comparison to untreated control groups, indicating that the symbiosis is relevant for the development of the entire colony.

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Phylogenetic Comparison of Endosymbionts with Their Host Ants Based on Molecular Evidence

Cited by 49 publications

References 25 publications

Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae: a model molecule for molecular systematic studies.

Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae: a model molecule for molecular systematic studies.

Bacterial Communities in the Midgut of Ponerine Ants (Hymenoptera: Formicidae: Ponerinae)

Relevance of the Endosymbiosis of Blochmannia floridanus and Carpenter Ants at Different Stages of the Life Cycle of the Host

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