Phylogenetic diversity of Mesorhizobium in chickpea

Abstract

Crop domestication, in general, has reduced genetic diversity in cultivated gene pool of chickpea (Cicer arietinum) as compared with wild species (C. reticulatum, C. bijugum). To explore impact of domestication on symbiosis, 10 accessions of chickpeas, including 4 accessions of C. arietinum, and 3 accessions of each of C. reticulatum and C. bijugum species, were selected and DNAs were extracted from their nodules. To distinguish chickpea symbiont, preliminary sequences analysis was attempted with 9 genes (16S rRNA, atpD, dnaJ, glnA, gyrB, nifH, nifK, nodD and recA) of which 3 genes (gyrB, nifK and nodD) were selected based on sufficient sequence diversity for further phylogenetic analysis. Phylogenetic analysis and sequence diversity for 3 genes demonstrated that sequences from C. reticulatum were more diverse. Nodule occupancy by dominant symbiont also indicated that C. reticulatum (60 percent) could have more various symbionts than cultivated chickpea (80 percent). The study demonstrated that wild chickpeas (C. reticulatum) could be used for selecting more diverse symbionts in the field conditions and it implies that chickpea domestication affected symbiosis negatively in addition to reducing genetic diversity.

Diversity and Effectivity of Indigenous Mesorhizobium Strains for Chickpea (Cicer arietinum L.) in Myanmar

“…In Ethiopia, the collected root nodule bacteria from chickpea were observed the genus Mesorhizobium by 16S rRNA gene [38]. These results were dependable with former reports that showed chickpea rhizobia were more closely associated to Mesorhizobium species [39,40]. The present study focused on the investigation of 16S rRNA region of 30 Mesorhizobial strains were successfully isolated from the different soil samples of major chickpea growing areas in Myanmar and proved as pure Mesorhizobium strains [19].…”

Adapting legume crops to climate change using genomic approaches

“…Reduction in genetic diversity of cultivated legumes compared with their wild relatives and ancestors has been discussed for different plants such as common bean (Bellucci et al, ; Bitocchi et al, ; Gepts, ), soybean (Hyten et al, ; Lam et al, ), narrow‐leafed lupin ( Lupinus angustifolius ; Berger, Buirchell, Luckett, & Nelson, ), and chickpea (Varshney et al, ). Interestingly, a study illustrated that reduction in genetic diversity as a result of domestication could go beyond the plant itself, as lower sequence variation was observed in rhizobia from domesticated chickpea compared with those from the wild type, which may suggest the potential negative impact of chickpea domestication on symbiosis (Kim et al, ). Shifts in genetic variation as a result of domestication, crop expansion, and breeding highlight the need for conserving and management of genetic resources for future breeding attempts.…”

Genetic Variability, Heritability and Genetic Advance Study for Seed Yield and Yield Component Traits in a Chickpea Recombinant Inbred Line (RIL) Population

“…Crop domestication under the influence of evolutionary forces and human derived selection process has resulted in several desirable traits in crops such as high productivity, short duration, reduced seed dispersal/ dormancy and branching, uniform flowering/ fruiting and seed maturation 1,2,3,4 .…”