Phylogenetic of ERIC-DNA Fingerprinting and New Sequencing of <i>Aeromonas</i> Species and <i>V. Cholerae </i>DNA

Abstract

The current study included 44 isolate of A. hydrophila, A. sobria and V. cholerae and other bacteria isolated from stool samples and environmental samples (Kufa river water and hospital environmental samples). ERIC DNA Fingerprinting with ERIC primers pairs generated distinct amplification bands ranging in size from (87 bp to 8000 kb). The 44 isolates produced 93 different patterns by ERIC DNA fingerprinting. The fingerprinting patterns of the isolates were constructed using cluster analysis the UPGMA (group method) using average linkages Number of different bands (Similarity coefficient), 1% tolerance. The PCR method of gene (16SrDNA and 16SrRNA) were the best methods for diagnosis, which has led to isolate and diagnosis of A. hydrophila A. sobria and V. cholerae are distributed as clinical isolates of A. hydrophila A. sobria were diarrhea samples. While, the environmental isolates were isolate of V. cholerae from Kufa river water. Sequencing technology is used to diagnosis of A. hydrophila, A. sobria and V. cholerae isolates were examined by (16SrDNA, 16SrRNA) genes. Recorded the new isolates in Nucleotide/Blast and recorded as the first sequencing in Gene-Bank/NCBI, DDBJ and ENA (INSDC). Each sequence have Accession number (No.: Gene bank: LC194875 Aeromonas sobria-HNK1, Gene bank: LC194876 Aeromonas hydrophila-HNK2, Gene bank: LC194877 Vibrio cholerae-HNK3) this is the first study in Iraq for discovery of new isolates by new sequences. The frequency of A. hydrophila, A. sobria and V. cholerae isolates in Najaf were higher among clinical and environmental isolates. The ERIC band pattern is an adequate tool for epidemiological investigations of A. hydrophila, A. sobria and V. cholerae isolates.

Molecular Profiling of Class I Integron Gene in MDR Salmonella typhi Isolates

“…Integrons are classified based on sequence similarity, there are at least five classes of integrons with class I integrons being the most studied and characterized 16 . Class 1 integrons are the most common and widely distributed among Gram-negative bacteria and are associated with functional and nonfunctional transposons 17,18 . Class 1 integron is composed of the(3"CS) and including 2 (ORFs)open reading frames.…”

“…-statistical service technology and Bioinformatics has advance of diagnostics and medical in (NGS) and ecological research. Bioinformatics specialized in Microbiology and set new standards for high level services of data analysis including high expertise in NGS18,24 . In this study is using Bioinformatics program to designing the specific primer of Class I intI gene in MDR Salmonella typhi.…”

Molecular detection and phylogenetic analysis of Vibrio cholerae genotypes in Hillah, Iraq

“…Phylogenetic trees have many functions; the generation of branching, treelike diagrams provides a way to visualize an estimated lineage of the genetic relationship among organisms, showing the ways families are derived during their evolution. In addition, the trees can help clarify the origins of an organism, its evolution pathway, its sequence similarity mutation correlation with other organisms and evolutionary distances [ 20 , 53 , 54 , 55 ].…”

ANTIBIOFILM FORMATION ACTIVITY, RESISTANT GENES PROFILING AND DETECTION OF VIRULENCE FACTORS OF TOXIGENICVibrio choleraeISOLATES FROM KISUMU COUNTY, KENYA

“…cholerae within the host [7]. TcpI is thought to be a chemo sensor and is proposed to be a sensor protein that negatively regulates tcpA [5]. ToxR dimers, but not monomers will bind to operator region of ctxAB operon and activate its transcription [8].…”

“…Besides CT and the pilus, other factors [other potential toxins, accessory colonization factors, outer membrane proteins, proteases, hemolysins, hemagglutinins (HAs), and a capsular polysaccharide] including those which are necessary for survival of the bacteria in vivo , penetration of the mucous layer and adherence to the underlying epithelial cells of the intestine, binding and internalization of CT, evasion of the host defense system, etc., may also contribute to the virulence contributing to survival and multiplication of V. cholerae within the host [7] . TcpI is thought to be a chemo sensor and is proposed to be a sensor protein that negatively regulates tcpA [5] . ToxR dimers, but not monomers will bind to operator region of ctxAB operon and activate its transcription [8] .…”