Phylogenetic relationships and evolutionary traits in Ranunculus s.l. (Ranunculaceae) inferred from ITS sequence analysis

Hörandl, Elvira; Paun, Ovidiu; Johansson, Jan Thomas; Lehnebach, Carlos A.; Armstrong, Tristan; Chen, Lixue; Lockhart, Peter J.

doi:10.1016/j.ympev.2005.02.009

Cited by 126 publications

(160 citation statements)

References 55 publications

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“…The phylogenetic studies carried out by means of cpDNA restriction sites (Johansson, 1998) and the ITS and cpDNA sequences (Hörandl et al, 2005;Paun et al, 2005a;Paun et al, 2005b;Lehnebach et al, 2007;Emadzade et al, 2011) demonstrate the need to separate some of the genera related to Ranunculus, including Ceratocephala, Ficaria and Myosurus of the Italian flora. In particular, Ficaria is substantiated by obvious synapomorphies: achenes with an elongate cuneate base and rudimentary beak, three sepals, strongly dimorphic roots and only one cotyledon (Förster, 1997).…”

Section: Ficariamentioning

confidence: 99%

Notes on systematics and taxonomy for the Italian vascular flora. 2.

Banfi¹,

Galasso²,

Soldano³

2011

Nat Hist Sci

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-The present study forms part of the series of systematic and taxonomic contributions related to plant species of the Italian flora, and also other phanerogams, in light of recent biosystematic works of a molecular-genetic nature.Caloscordum and Nectaroscordum are separated from Allium s.l., Holandrea is included within Dichoropetalum, Ficaria is separated from Ranunculus and Althaea sect. Hirsutae is included within Malva. Confirmation is given for the inclusion of Lavatera within Malva, of Aegilops within Triticum and for the separation of Phelipanche from Orobanche. In addition some observations regarding the genera Cytisus, Hippophaë, Portulaca, Rhaponticum, Senecio and Torilis are presented.Finally new combinations and/or new names are proposed within the genera Caloscordum, Nectaroscordum, Dichoropetalum, Ficaria, Hippophaë, Malva, Portulaca and Rhaponticum.Key words: Italian vascular flora, systematics, taxonomy, nomenclature.Riassunto -Appunti di sistematica e tassonomia per la flora italiana. 2. Continua la serie di contributi sistematici e tassonomici relativi a entità della flora italiana, ma anche ad altre fanerogame, predisposti alla luce di recenti lavori di biosistematica su base geneticomolecolare.Da Allium s.l. vengono separati Caloscordum e Nectaroscordum, Holandrea è unito a Dichoropetalum, Ficaria è separato da Ranunculus e Althaea sect. Hirsutae è unita a Malva. Viene ribadita la riunione di Lavatera a Malva, di Aegilops a Triticum e la separazione di Phelipanche da Orobanche. Inoltre vengono fatte alcune considerazioni sui generi Cytisus, Hippophaë, Portulaca, Rhaponticum, Senecio e Torilis.Infine vengono proposte nuove combinazioni e/o nuovi nomi nei generi Caloscordum, Nectaroscordum, Dichoropetalum, Ficaria, Hippophaë, Malva, Portulaca e Rhaponticum.

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Section: Ficariamentioning

confidence: 99%

Notes on systematics and taxonomy for the Italian vascular flora. 2.

Banfi¹,

Galasso²,

Soldano³

2011

Nat Hist Sci

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“…The ITS region (ITS1, 5.8S and ITS2) was amplified as a single piece with primers 17SE and 26SE (Sun et al 1994;Hörandl et al 2005). Amplification of selected regions was carried out in 25 µL of reaction mixture (25 ng template DNA, 2.5 µL of 10× PCR buffer, 2.5 mM MgCl2, 0.2 mM dNTPs, 0.08 µM of each primer and 1 U of Taq DNA polymerase, Invitrogen), in an Eppendorf Mastercycler Epgradient S (Westbury, NY).…”

Section: Nuclear Rdna (Its) Amplification and Sequencingmentioning

confidence: 99%

Molecular characterization of two natural hybrids from the Iberian Peninsula: Ranunculus × luizetii and R. × peredae (Ranunculaceae)

2012

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Two nothospecies, Ranunculus ×luizetii and R. ×peredae (Ranunculaceae), were analyzed and discussed. For this purpose, Amplified Fragment Length Polymorphism (AFLP) markers, nuclear rDNA sequences (ITS1, 5.8S and ITS2) and pollen viability were conducted. The profiles of these hybrid samples were compared to their putative progenitors. Several additive polymorphic sites detected in the ITS sequences of the hybrid samples (R. ×luizetii and R. ×peredae) also confirmed their derived origins from ribotypes of their parental taxa (R. parnassiifolius subsp. parnassiifolius × R. pyrenaeus; R. amplexicaulis × R. cabrerensis subsp. cabrerensis, respectively). Despite the lack of exclusive AFLP markers reported in both hybrids, presumably due to effects of introgression, the concerted evolution of many rDNA polymorphisms towards either of the parental ribotypes indicated their ancient origin. Pollen fertility estimation in R. ×luizetii presented a mean value of 60.58%, which showed that hybrid samples are well established and fertile. However, a larger difference was observed in R. ×peredae, where the mean value of pollen fertility was very low (18.91%).

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“…However, there are few examples of applications of using network programs at the angiosperm species-level. For instance, hybrid relationships in Opuntia were investigated with the Median Network approach of Spectronet (Griffith, 2003), and split decomposition (with the program SplitsTree) was used to study species radiation and reticulate relationships in Ranunculus (Lockhart & al., 2001;Hörandl & al., 2005). Also, Median-Joining networks (Bandelt & al., 1999) have been used for detailed analyses of introgression and hybridization zones between two species of Populus (Lexer & al., 2005) and Passiflora (LorenzLemke & al., 2005).…”

Section: Current Tools For Repre-senting Incongruent Phylo-genetic Pamentioning

confidence: 99%