Phylogenetic studies of three sinipercid fishes (Perciformes: Sinipercidae) based on complete mitochondrial DNA sequences

Abstract

The sinipercids are a group of 12 species of freshwater percoid fish endemic to East Asia and their phylogenetic placements have perplexed generations of taxonomists. We cloned and sequenced the complete mitochondrial DNA (mtDNA) of three sinipercid fishes (Siniperca chuatsi, S. kneri, and S. scherzeri) to characterize and compare their mitochondrial genomes. The mitochondrial genomes of S. chuatsi, S. kneri, and S. scherzeri were 16,496, 17,002, and 16,585 bp in length, respectively. The organization of the three mitochondrial genomes is similar to those reported from other fish mitochondrial genomes, which contains 37 genes (13 protein-coding genes, 2 ribosomal RNAs, and 22 transfer RNAs) and a major non-coding control region. Among the 13 protein-coding genes of all the three sinipercid fishes, three reading-frame overlaps were found on the same strand. There is an 81-bp tandem repeat cluster at the end of CSB-3 in the S. scherzeri control region. The complete mitochondrial genomes of the three sinipercids should be useful for the evolutionary studies of sinipercids and other vertebrate species.

Complete mitochondrial genome sequence of Hemiculter bleekeri

“…The total length of the complete mitochondrial genome was 16,617 bp in length which included 13 protein-coding genes, 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes and one control region (D-loop). The gene arrangement and transcriptional direction were similar to those seen in typical teleosts mitogenomes (Li et al 2010;Chen et al 2012). Most genes were encoded on the H-strand, except for ND6 gene and eight tRNA genes that were encoded on the L-strand.…”

“…Mitochondria are doublelayered membrane-bound organelles, found in nearly all eukaryotic cells with an estrangement genetic affinity. In addition, the mitochondria have some unique characteristics, such as small size (16K), high abundance, maternal inheritance, lack of recombination and rapid evolutionary rate (Chen et al 2012). It has been considered as a natural marker and widely applied in population genetics and evolutionary studies (Rand 2008;Su et al 2014).…”

Inheritance and phylogenetic analysis of Tylosurus acus melanotus based on the complete mitochondrial genomes

“…The genomic DNA was stored in À20 C. The total length of the mitochondrial genome is 16,723 bp and deposited in the GenBank with accession number KU605633. The gene arrangement and genome size of the mitochondrial genomes were similar to other bony fishes (Chen et al 2012;Li et al 2010). The mitochondrial DNA (mtDNA) was composed of 13 proteincoding genes, 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes and 1 control region (D-loop).…”

“…Mitochondria are double-layered membrane-bound organelles with some unique characteristics, including maternal inheritance, small size (16K), lack of recombination and high evolutionary rate (Chen et al 2012). The mitogenome has been widely considered as a natural bio-marker for population genetic and evolutionary studies (Su et al 2014).…”

Simple sequence repeat-based analysis of the genetic diversity and population genetic structure of populations of Siniperca chuatsi

“…It is an important freshwater fish species in China, and is in high demand by domestic and international markets (Zhao et al, 2008;Chen et al, 2012). S. chuatsi has been farmed since the early 1970s in several provinces in China, including Guangdong, Jiangsu, Hubei, and Jianxi.…”