Physical and biological effects of gellan gum on decreasing postoperative adhesion in a rat model

Abstract

An antiadhesion membrane made from gellan gum was fabricated and characterized. A 12-µm-thick membrane of gellan gum was prepared and reacted with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide to obtain a cross-linked membrane (G/A70) with 87% gel content and a tensile strength of 46.5 MPa. In vivo, the G/A70 membrane had 90% less tissue adhesion. Inflammation-related and extracellular matrix protein gene expression in a rat model of abdominal surgery was found by real-time quantitative reverse transcription polymerase chain reaction analysis. On day 3, after surgery, the gene expression of ceruloplasmin and type V collagen in the G/A70-treated group was 1.9 and 0.3 times that of the control group, respectively. The G/A70 membrane elicited mild inflammation but suppressed type V collagen synthesis and reduced the occurrence of tissue adhesion. These findings provide insights into the properties of gellan gum antiadhesion membranes and help to overcome problems involving tissue adhesions in surgical procedures.

Gellan gum/clay hydrogels for tissue engineering application: Mechanical, thermal behavior, cell viability, and antibacterial properties

“…It is also used as a scaffold material for tissue engineering application. 14,15 GG has been reported to have good biocompatibility on mouse fibroblast cells (L929), 16 human skin fibroblast cells (CRL2522), 17 and human fetal osteoblasts (hFOBs 1.19). 18 In general, hydrogel can be classified as a three dimensional polymer network which has received more attention in past decade due to its ability to absorb and retain high amount of water.…”

Bonding behavior of hydrophobically modified gelatin films on the intestinal surface

“…[1][2][3] Among them, material-based barriers, especially anticoagulation film-based barriers, have a high reliability for preventing post-surgical agglutination. [4][5][6][7] Some commercially available barrier materials include Seprafilm ® , [8][9][10][11][12][13] Intercede ® , 14 Repel-CV ® , 15 Gore-Tex Surgical Membrane ® , 16 and Prevadh ® . 17 These barrier materials are normally composed of cellulose derivatives, hyaluronic acid, poly(ethylene glycol), poly(lactic acid), hydrophilic collagen, or polytetrafluoroethylene.…”