Physical Map of the DNA Genome of
            <i>Autographa californica</i>
            Nuclear Polyhedrosis Virus

Miller, Lois K.; Dawes, Katherine P.

doi:10.1128/jvi.29.3.1044-1055.1979

Cited by 76 publications

(23 citation statements)

References 19 publications

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“…Second, later in the infectious cycle, production of NOV is curtailed and occlusion bodies (OB), which consist of enveloped bundles of nucleocapsids lying within a paracrystalline protein matrix, form in the cell nuclei. The genome of AcNPV consists of a double-stranded, superhelical, circular DNA molecule of approximately 128 kilobase pairs which has been physically mapped for several restriction enzyme sites (9,12,17). Wild isolates of AcNPV derived from insects can be separated by plaque purification into variant strains having minor alterations in DNA restriction patterns (8,11,15).…”

mentioning

confidence: 99%

Molecular Cloning and Physical Mapping of Restriction Endonuclease Fragments of Autographa californica Nuclear Polyhedrosis Virus DNA

Cochran

Carstens

Eaton

et al. 1982

J Virol

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A restriction fragment library containing Autographa californica nuclear polyhedrosis virus (AcNPV) DNA was constructed by using the pBR322 plasmid as a vector. The library, which is representative of more than 95% of the viral genome, consists of 2 of the 7 BamHI fragments, 12 of the 24 HindIII fragments, and 23 of the 24 EcoRI fragments. The cloned fragments were characterized and used to generate physical maps of the genome by hybridizing nick-translated recombinant plasmid to Southern blots of AcNPV DNA digested with SmaI, BamHI, XhoI, PstI, HindIII, and EcoRI restriction endonucleases. This information was used to define our strain of AcNPV (HR3) with respect to other strains for which physical maps have been previously published. The hybridization data also indicate that reiteration of DNA sequences occurs at the HindIII-L and -Q regions of the genome.

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mentioning

confidence: 99%

Molecular Cloning and Physical Mapping of Restriction Endonuclease Fragments of Autographa californica Nuclear Polyhedrosis Virus DNA

Cochran

Carstens

Eaton

et al. 1982

J Virol

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“…The restriction map sites are a combination of the sites determined by Miller and Dawes for L-1 (14) and the sites determined by Cochran et al for HR (2). The fragment letter designations are similar to those described by Cochran et al (14), except that the Hindlll fragment in the 40to 50-map unit region is designated HindIII-A and the two HindIll fragments in the 70to 85-map unit region are designated B1 and B2. The assignment of Bi and B2 to specific AcNPV L-1 HindIll fragments is shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Particularly advantageous is that each cDNA selects only a single mRNA or a series of related spliced or symmetrically transcribed RNAs. In this paper we report the successful synthesis and cloning of DNA complementary to late AcNPV mRNA. Using cDNA clones, we gathered information concerning the relative amounts of various mRNAs found late in infection, the locations of these mRNAs with respect to the physical map of AcNPV L-1 (2,14), and, in some cases, the identities of the proteins which the mRNAs encode. The physical map location of the 3' portion of the gene encoding polyhedrin, the major protein of the occluded form of AcNPV, was determined.…”

mentioning

confidence: 99%

Molecular cloning of DNA complementary to mRNA of the baculovirus Autographa californica nuclear polyhedrosis virus: location and gene products of RNA transcripts found late in infection

Adang¹,

Miller²

1982

J Virol

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DNAs complementary to late Autographa californica nuclear polyhedrosis virus (AcNPV) mRNA were synthesized by reverse transcription and cloned in Escherichia coli by using pBR322 as a vector. Eleven different cDNAs were distinguished in our screening of 45 AcNPV-homologous clones. Location of the regions of cDNA homology with respect to the AcNPV physical map showed that the 11 cDNAs were dispersed throughout the genome. The most abundant cDNA insertion, representing approximately one-third of the late viral mRNAs, was homologous to the AcNPV HindIII-P,Q and EcoRI-P fragments. The direction of transcription in this region was from left to right on a linearized AcNPV physical map. Hybridization selection followed by in vitro translation showed that this region encoded a 7,200-dalton (7.2K) protein which comigrated with a minor protein found in the extracellular nonoccluded form of the virus (NOV). Similarly, the gene for polyhedrin, the major structural protein of the occluded virus form, was located, at least in part, in the HindIII-V/EcoRI-I region of the AcNPV map. The polyhedrin transcript represented approximately one-quarter of the viral polyadenylic acid-containing RNAs at 27 h postinfection. Another relatively abundant cDNA was homologous to the HindIII-AIEcoRI-CISstI-G region, and t Research paper 8256 of the Idaho Agricultural Experiment Station. bridization to the DNA fragment, and determine the protein encoded in the nucleic acid sequences by in vitro translation of the hybridselected RNA followed by gel electrophoresis of the resulting protein products. Using two AcNPV genomic fragments as hybridization probes, Vlak et al. have mapped 33,000-dalton (33K) and 39K proteins to the EcoRI-I and EcoRI-J fragments, respectively (23). Recent advances in recombinant DNA technology have provided methods for cloning the coding regions of individual virus genes by synthesizing DNA complementary to mRNA, using reverse transcriptase. The use of cDNA clones as hybridization probes for specific mRNAs has distinct advantages over the use of viral genomic 782

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“…Baculoviruses are a diverse group of common insect pathogens that primarily infect the order Lepidoptera. These viruses contain circular double-stranded DNA genomes of 90 to 160 kb (6,30,38,41). They infect insect cells productively and generate numerous viral progenies.…”

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confidence: 99%

Persistent Baculovirus Infection Results from Deletion of the Apoptotic Suppressor Gene p35

1998

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Infection with the wild-type baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) results in complete death of Spodoptera frugiperda (Sf) cells. However, infection of Sf cells with AcMNPV carrying a mutation or deletion of the apoptotic suppressor gene p35 allowed the cloning of surviving Sf cells that harbored persistent viral genomes. Persistent infection established with the virus with p35 mutated or deleted was blocked by stable transfection of p35 in the host genome or by insertion of the inhibitor of apoptosis (iap) gene into the viral genome. These artificially established persistently virus-infected cells became resistant to subsequent viral challenge, and some of the cell lines carried large quantities of viral DNA capable of early gene expression. Continuous release of viral progenies was evident in some of the persistently virus-infected cells, and transfection of p35 further stimulated viral activation of the persistent cells, including the reactivation of viruses in those cell lines without original continuous virus release. These results have demonstrated the successful establishment of persistent baculovirus infections under laboratory conditions and that their establishment may provide a novel continuous, nonlytic baculovirus expression system in the future.

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Physical Map of the DNA Genome of Autographa californica Nuclear Polyhedrosis Virus

Cited by 76 publications

References 19 publications

Molecular Cloning and Physical Mapping of Restriction Endonuclease Fragments of Autographa californica Nuclear Polyhedrosis Virus DNA

Molecular Cloning and Physical Mapping of Restriction Endonuclease Fragments of Autographa californica Nuclear Polyhedrosis Virus DNA

Molecular cloning of DNA complementary to mRNA of the baculovirus Autographa californica nuclear polyhedrosis virus: location and gene products of RNA transcripts found late in infection

Persistent Baculovirus Infection Results from Deletion of the Apoptotic Suppressor Gene p35

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