Physical properties of NiCl2 coated protein chip plates

Hyun, June Won; Yun, Mi Young; Noh, S. J.; Ahn, Yonghyun; Huh, Young‐Duk; Park, Heonyong; Pyee, Jaeho; Ji, Hye-Goo

doi:10.1016/j.cap.2005.07.056

Cited by 4 publications

(3 citation statements)

References 5 publications

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“…Hyun et al (2002) developed the nickel chloride-coated protein chip by spin-coating 11% (w/w) of NiCl 2 on the surface of glass slide to absorb the His-tagged protein. Later, the physical properties of this protein chip were examined (Hyun et al, 2006). Agarwal et al (2003) utilized the dip-pen nanolithography (DPN) technique to immobilize the His-tagged protein on a metallic nickel surface.…”

Section: Introductionmentioning

confidence: 99%

Protein microarray chip with Ni–Co alloy coated surface

Chang

2010

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

Protein microarray chip with Ni–Co alloy coated surface

Chang

2010

Biosensors and Bioelectronics

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“…Based on this mature technique, the research on protein chips has evoked great attention [7,8] and many kinds of protein chips were developed. In 2002, Hyun et al [9,10] directly deposited Ni chemical composition onto glass slides to capture His protein. Cheng et al [11], in 2004, reported that a unique peptide sequence of HGGHHG showed a good chelating ability to bind a transition metal on a chip better than hexa-His peptide and, thus, this chip can be used as a high throughput technique for protein array.…”

Section: Introductionmentioning

confidence: 99%

Immunoassay chip for URICASE protein using histidine-immobilization technique

Chang

2008

2008 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems

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This paper presents a nickel-coated immunoassay chip fabricated by electroplating technology for Uricase protein. The Taguchi method was adopted to determine the optimal electroplating condition. The considered factors that affect the performance of nickel-coated chip included current density of electroplating, operation temperature, and pH of electroplating buffer, etc. As a result of systematic electroplating approach, a stable and optimal chip surface was obtained. Due to the physical adsorption between intermediate metal and protein, this chip provides specific binding with His-tagged Uricase. In addition, it offers self-assembling and directional molecule immobilization on the surface of chip. The immunoassay was performed for verification. This nickel-coated chip can be used not only for His-tagged Uricase but for any His-tagged proteins in the pathological analyses with inexpensive microarray chip design.

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“…The immobilisation mechanism is based on the technique of immobilised metal affinity chromatography (IMAC). Most protein microarrays based on the theory of IMAC were fabricated by using the chelator or compound of monometallic ion, such as Ni +2 , to capture the histidine-tagged protein [14][15][16]. However, the protein immobilisation layer of this proposed droplet-based protein chip possesses bi-metallic elements, that is, by means of adding cobalt to nickle to enhance the specific binding capability to immobilise functional proteins with the His-tag attached [17].…”

mentioning

confidence: 99%

Droplet-based protein chip with Ni–Co coated surface

Zhang

Chang²,

Ding

et al. 2013

Micro Nano Lett.

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This Letter describes the implementation of a droplet-based protein chip with an Ni-Co alloy layer for conducting immunoassays. The proposed chip consists of a substrate of a printed circuit board and a microchannel layer. On the substrate surface, an Ni-Co coating was fabricated by electrodeposition to immobilise the histidine-tagged protein. Through droplet fusion and mixing, the fused droplets aligned at the downstream position of the microchannel and were immobilised by the Ni-Co layer for further incubation and fluorescence detection. The experimental results showed that the sample which is encapsulated by the carrier fluid does not have evaporation and contamination problems. Therefore strong fluorescence intensity proportional to the sample concentration can be obtained. The fluorescence intensity is also related to the droplet size. An extremely low concentration of encapsulated content in a smaller droplet might result in arduous fluorescence detection. This proposed droplet-based protein chip allows the operation sequence of the immunoassay to be conducted automatically through the manipulation of droplets. Moreover, the fabrication process is simple and the chip can be implemented to immobilise any functional proteins with His-tag attached.

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Physical properties of NiCl2 coated protein chip plates

Cited by 4 publications

References 5 publications

Protein microarray chip with Ni–Co alloy coated surface

Protein microarray chip with Ni–Co alloy coated surface

Immunoassay chip for URICASE protein using histidine-immobilization technique

Droplet-based protein chip with Ni–Co coated surface

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