Physico-Chemical Characterization and &lt;i&gt;In Vitro&lt;/i&gt; Biological Evaluation of Pure SiHA for Bone Tissue Engineering Application

Marchat, David; Bouet, Guénaëlle; Ludeckgen, Aline; Zymelka, Maria; Malaval, Luc; Szenknect, Stéphanie; Dacheux, Nicolas; Bernache‐Assollant, Didier; Chevalier, Jérôme

doi:10.4028/www.scientific.net/kem.529-530.351

Cited by 4 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this field, silicon is known to influence the strength, the formation and the calcification of the bone tissue [7,8], and this has led to the development of silicon containing biomaterials [9]. Among these biomaterials particular attention has been given to silicated hydroxyapatite (SiHA, Ca 10 (PO 4 ) 6−x (SiO 4 ) x (OH) 2−x ) ceramics [10][11][12], whose biological properties are currently being investigated [13][14][15]. Functionalizing the surface with specific biomolecules [16] could increase HA ceramics biointegration by stimulating the behavior of the cells involved in angiogenesis and/or osteogenesis.…”

Section: Introductionmentioning

confidence: 99%

Functionalization of Hydroxyapatite Ceramics: Raman Mapping Investigation of Silanization

et al. 2019

View full text Add to dashboard Cite

Surface modification of bioceramic materials by covalent immobilization of biomolecules is a promising way to improve their bioactivity. This approach implies the use of organic anchors to introduce functional groups on the inorganic surface on which the biomolecules will be immobilized. In this process, the density and surface distribution of biomolecules, and in turn the final biological properties, are strongly influenced by those of the anchors. We propose a new approach based on Raman 2D mapping to evidence the surface distribution of organosilanes, frequently used as anchors on biomaterial surfaces on hydroxyapatite and silicated hydroxyapatite ceramics. Unmodified and silanized ceramic surfaces were characterized by means of contact angle measurements, atomic force microscopy (AFM) and Raman mapping. Contact angle measurements and AFM topographies confirmed the surface modification. Raman mapping highlighted the influence of both the ceramic’s composition and silane functionality (i.e., the number of hydrolysable groups) on the silane surface distribution. The presence of hillocks was shown, evidencing a polymerization and/or an aggregation of the molecules whatever the silane and the substrates were. The substitution of phosphate groups by silicate groups affects the covering, and the spots are more intense on SiHA than on HA.

show abstract

Section: Introductionmentioning

confidence: 99%

Functionalization of Hydroxyapatite Ceramics: Raman Mapping Investigation of Silanization

et al. 2019

View full text Add to dashboard Cite

show abstract

“…For instance in another study of Kumar et al, is the osteogenic differentiation of hBMSCs, seeded on etched poly(ε-caprolactone) scaffolds, induced by surface roughness or surface chemistry change (112) ? It is also unfortunate that series of studies on in vivo and in vitro evaluation of silicon-substituted hydroxyapatite ceramics would have been published without any information about the real phase composition of the samples (110,113), when it was recently shown that even small changes of silicon-substituted hydroxyapatite ceramic phase composition drastically modify cellular responses (114,115).…”

Section: Future Challenges and Strategymentioning

confidence: 99%

In VitroThree-Dimensional Bone Tissue Models: From Cells to Controlled and Dynamic Environment

Bouet

Marchat

Cruel

et al. 2015

Tissue Engineering Part B: Reviews

Self Cite

View full text Add to dashboard Cite

Most of our knowledge of bone cell physiology is derived from experiments carried out in vitro on polystyrene substrates. However, these traditional monolayer cell cultures do not reproduce the complex and dynamic three-dimensional (3D) environment experienced by cells in vivo. Thus, there is a growing interest in the use of 3D culture systems as tools for understanding bone biology. These in-vitro-engineered systems, less complex than in vivo models, should ultimately recapitulate and control the main biophysical, biochemical, and biomechanical cues that define the in vivo bone environment, while allowing their monitoring. This review focuses on state-of-the-art and the current advances in the development of 3D culture systems for bone biology research. It describes more specifically advantages related to the use of such systems, and details main characteristics and challenges associated with its three main components, that is, scaffold, cells, and perfusion bioreactor systems. Finally, future challenges for noninvasive imaging technologies are addressed.

show abstract

“…Hydroxyapatite (HA) is among the most widely used bone replacement materials due to its strong affinity with the mineral component of bones; it possesses good bioactivity, osteoconductivity and biocompatibility with the human bone tissue. 1,2 Although, stoichiometric Hydroxyapatite (HA) -Ca 10 (PO 4 ) 6 (OH) 2 has been a widely used model for the apatite present in the bone tissues for many years, the chemical composition of biological apatites differs from the stoichiometric HA. 3 The biological apatites are uniquely similar in that they all comprise carbonate in varying amounts of 2-8 wt.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Biocompatibility of SiCHA Nanopowders on Human Mesenchymal Stem Cells

Ismail¹,

Wimpenny²,

Bretcanu³

et al. 2018

JES

View full text Add to dashboard Cite

Synthetic hydroxyapatite (HA) possesses good biocompatibility, bioactivity and osteoconductivity and closely mimics the mineralised phase of human bone and teeth. However, for many years the clinical researchers worldwide have been aware that the mineral phase of bone is not solely HA but contains a number of substituents (e.g. CO 3 , Si, Zn, Sr, Na and Mg). Among the substituents, carbonate is the major substitute ions which are present about 2-8 wt. % depending mainly on the individual age. The presence of approximately 1 wt. % Si is known to be essential in bone formation and calcification. Thus, the present study is aimed at developing a new biomaterial consisting both of these two biologically important cations into the HA structure by synthesising silicon carbonated HA (SiCHA) powders via nanoemulsion method, and to assess the in vitro biocompatibility of the as-synthesised powders in response to human Bone Marrow derived Mesenchymal Stem Cells (hMSCs). Both the carbonate and silicon ions were successfully substituted into the HA lattice. Powders with carbonate encouraged better cell activity in comparison to the carbonate-free powders. While, SiHA and SiCHA-1 as-synthesised powders with high amount of Si (> 1 wt. %) tend to kill some cells at the early stage of seeding and hinder cells activity afterwards. Among the tested powders, SiCHA-2 as-synthesised powders is chosen as the optimum composition as it exhibited the highest cell viability and proliferation besides having the closest amount of carbonate and Si ions to the bone mineral.

show abstract

Physico-Chemical Characterization and <i>In Vitro</i> Biological Evaluation of Pure SiHA for Bone Tissue Engineering Application

Cited by 4 publications

References 13 publications

Functionalization of Hydroxyapatite Ceramics: Raman Mapping Investigation of Silanization

Functionalization of Hydroxyapatite Ceramics: Raman Mapping Investigation of Silanization

In VitroThree-Dimensional Bone Tissue Models: From Cells to Controlled and Dynamic Environment

In Vitro Biocompatibility of SiCHA Nanopowders on Human Mesenchymal Stem Cells

Contact Info

Product

Resources

About