Physico-chemical studies of molecular interactions between non-ionic surfactants and bovine serum albumin

Ruiz-Peña, Miriam; Oropesa‐Nuñez, Reinier; Pons, Tirso; Louro, Sônia R.W.; Pérez‐Gramatges, Aurora

doi:10.1016/j.colsurfb.2009.08.046

Cited by 97 publications

(50 citation statements)

References 35 publications

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“…There was no significant difference in the EC of polysorbate 20, polysorbate 80 and soy lecithin. This is not in accordance with the study by Ruiz-Peña et al (2010) who reported that polysorbate 80 had higher value of surface excess concentration at surface saturation compared to polysorbate 20. High value of surface excess concentration at surface saturation indicated high effectiveness of adsorption at surface, thereby giving high emulsifying capacity.…”

Section: Emulsifying Capacity Of Emulsifierscontrasting

confidence: 55%

Characterization of flaxseed oil emulsions

Lee

Choo

2014

J Food Sci Technol

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The emulsifying capacity of surfactants (polysorbate 20, polysorbate 80 and soy lecithin) and proteins (soy protein isolate and whey protein isolate) in flaxseed oil was measured based on 1 % (w/w) of emulsifier. Surfactants showed significantly higher emulsifying capacity compared to the proteins (soy protein isolate and whey protein isolate) in flaxseed oil. The emulsion stability of the flaxseed oil emulsions with whey protein isolate (10 % w/w) prepared using a mixer was ranked in the following order: 1,000 rpm (58 min)≈ 1,000 rpm (29 min) ≈ 2,000 rpm (35 min) >2,000 rpm (17.5 min). The emulsion stability of the flaxseed oil emulsions with whey protein isolate (10 % w/w) prepared using a homogenizer (Ultra Turrax) was independent of the speed and mixing time. The mean particle size of the flaxseed oil emulsions prepared using the two mixing devices ranged from 23.99±1.34 μm to 47.22±1.99 μm where else the particle size distribution and microstructure of the flaxseed oil emulsions demonstrated using microscopic imaging were quite similar. The flaxseed oil emulsions had a similar apparent viscosity and exhibited shear thinning (pseudoplastic) behavior. The flaxseed oil emulsions had L* value above 70 and was in the red-yellow color region (positive a* and b* values).

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Section: Emulsifying Capacity Of Emulsifierscontrasting

confidence: 55%

Characterization of flaxseed oil emulsions

Lee

Choo

2014

J Food Sci Technol

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“…SDS and Tween-20 were chosen as points of comparison because their interactions with BSA are well characterized [46][47][48]. SDS and Tween-20 are also structurally similar to POPC and PEGylated lipids (Supporting Information Fig.…”

Section: Comparison Of Popc/dspeg3000 Coating With Anionic and Neutramentioning

confidence: 99%

Dynamic coating of SU‐8 microfluidic chips with phospholipid disks

et al. 2010

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In this work, PEG-stabilized phosphatidylcholine lipid aggregates (disks), mimicking mammalian cell membranes, were introduced as a new biofouling resistant coating for SU-8 polymer microchannels. A rapid and simple method was developed for immobilization of PEGylated phosphatidylcholine disks in microchannels. Microfluidic chips made from SU-8, PDMS, or glass were dynamically coated with the PEGylated disks followed by characterization of their surface chemistry before and after coating. On the basis of the observed changes in EOF and nonspecific protein adsorption, the affinity of the PEGylated disks was shown to be particularly strong toward SU-8. The PEG-lipid coating enabled permanent change in EOF in SU-8 microchannels with an initial value of 4.5 x 10(-8) m(2) V(-1) s(-1), decreasing to 2.1 x 10(-8) m(2) V(-1) s(-1) (immediately after modification), and, eventually, to 1.5 x 10(-8) m(2) V(-1) s(-1) (7 days after modification) for 9 mM sodium borate (pH 10.5) as BGE. As determined by the Wilhelmy plate measurements and microchip-CE analysis of BSA, the PEG-lipid coating also enabled efficient biofouling shield against protein adsorption, similar to that of low amounts of SDS (3.5 mM) or Tween-20 (80 microM) as buffer additives. These results suggest that dynamically attached PEG-lipid aggregates provide stable, biomimicking surface modification that efficiently reduces biofouling on SU-8.

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“…To use them correctly, it is necessary to have an idea of how and in which amounts they interact with proteins. Although surfactant-protein interactions have been widely studied for half a century [1][2][3][4][5], the mechanism of interaction is not well understood. Knowledge of the interactions is not only fundamental in theoretics, but also practical in industrial applications.…”

Section: Introductionmentioning

confidence: 99%

Interaction of bovine serum albumi with two alkylimidazolium-based ionic liquids investigated by microcalorimetry and circular dichroism

Zhu¹,

Li²,

Zheng³

2011

JBPC

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The interactions of bovine serum albumin (BSA) with two alkylimidazolium-based ionic liquids, 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim]BF 4 ) and 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim]PF 6 ), in buffer solutions at pH 7.0 were investigated by isothermal titration calorimetry (ITC) and circular dichroism (CD). CD spectra showed that the two ionic liquids changed the secondary structure of BSA. Data process was based on the supposition that there were several independent types of binding sites on each BSA molecule for the two ligand molecules. The results obtained by using this supposition combined with Langmuir adsorption model showed that there were two types of such binding sites. One was the high affinity binding site, and the other was the low affinity binding site. The binding constants, changes in enthalpy, entropy and Gibbs free energy for the two types of binding were obtained, which showed that the two types of binding were driven by a favorable entropy increase. Furthermore, for either the ionic liquids, the number of the high affinity binding sites is much smaller than that of the low affinity ones. These results were interpreted with the molecular structure of BSA and the different substituent groups on imidazole ring of the two ionic liquid molecules.

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Physico-chemical studies of molecular interactions between non-ionic surfactants and bovine serum albumin

Cited by 97 publications

References 35 publications

Characterization of flaxseed oil emulsions

Characterization of flaxseed oil emulsions

Dynamic coating of SU‐8 microfluidic chips with phospholipid disks

Interaction of bovine serum albumi with two alkylimidazolium-based ionic liquids investigated by microcalorimetry and circular dichroism

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