Physicochemical and Biochemical Characterization of Human α1-Microglobulin Expressed in Baculovirus- Infected Insect Cells

Perfusion of human placenta with hemoglobin introduces preeclampsia-like injuries that are prevented by α1-microglobulin

“…The separated proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon-P, Millipore, Bedford, MA, USA). The PVDF membranes were then incubated over-night as described [37] with mouse monoclonal anti-A1M antibodies (BN11.10, 10 µg/ml) [38], followed by incubation with 125 I-labelled rabbit anti-mouse IgG (10 ng/ml; Dako, Denmark). The membranes were developed in a Fuji FLA3000 phosphoimaging system (Fujifilm Sweden AB, Stockholm, Sweden).…”

Vitreous levels of oxidative stress biomarkers and the radical-scavenger α1-microglobulin/A1M in human rhegmatogenous retinal detachment

“…The different forms of A1M in the vitreous samples were analyzed by Western blotting, performed as described previously [34]. Electrophoresis was performed as described above.…”

Up-regulation of α1-microglobulin by hemoglobin and reactive oxygen species in hepatoma and blood cell lines

“…The separated proteins were transferred to polyvinylidenedifluoride (PVDF) membranes (Immobilon, Millipore, Bedford, MA, USA) as described [34]. The membranes were then incubated with the appropriate antibodies and Western blot was performed using 125 I-labelled secondary antibodies as previously described by Wester et al [35], and developing the images on the membranes using Fuji FLA 3000 phosphoimaging system (Fujifilm Sweden AB, Stockholm, Sweden).…”