Physicochemical and Biological Properties of Gelatin Extracted from Marine Snail Rapana venosa

Abstract

In this study, we aimed to obtain gelatin from the marine snail Rapana venosa using acidic and enzymatic extraction methods and to characterize these natural products for cosmetic and pharmaceutical applications. Marine gelatins presented protein values and hydroxyproline content similar to those of commercial mammalian gelatin, but with higher melting temperatures. Their electrophoretic profile and Fourier transform infrared (FTIR) spectra revealed protein and absorption bands situated in the amide region, specific for gelatin molecule. Scanning electron microscopy (SEM) analysis showed significant differences in the structure of the lyophilized samples, depending on the type of gelatin. In vitro studies performed on human keratinocytes showed no cytotoxic effect of acid-extracted gelatin at all tested concentrations and moderate cytotoxicity of enzymatic extracted gelatin at concentrations higher than 0.5 mg/mL. Also, both marine gelatins favored keratinocyte cell adhesion. No irritant potential was recorded as the level of IL-1α and IL-6 proinflammatory cytokines released by HaCaT cells cultivated in the presence of marine gelatins was significantly reduced. Together, these data suggest that marine snails are an alternative source of gelatins with potential use in pharmaceutical and skincare products.

Biomaterials from the sea: Future building blocks for biomedical applications

“…Marine gelatins possess high protein values (91.5% for acid-soluble gelatin and 83.1% for pepsin-soluble gelatin). The hydroxyproline content of marine gelatin is similar to that of commercial mammalian gelatin but with higher melting temperatures [ 144 ]. Some marine gelatin such as those derived from saithe ( Pollachius virens ) skin has a considerable mineral content (>20%) compared to the commercially available gelatin (<0.2%) [ 145 ].…”

Progress in Modern Marine Biomaterials Research

“…Gelatin (Table 9) can serve as cell carrier to repair tissue defects, i.e., gelatin extracted from marine snail Rapana venosa was reported as a biocompatible template for the growth of human keratinocytes [289]. Hence, this marine biomaterial can be used in tissue engineering, often in combination with other materials such as chitosan and silk fibroin [42].…”

Comparative Quality of Proteins and Morphological Structures of Gelatin From Sheepskin With Acid and Alkaline Treatment

“…When linked between gelatin microstructure with protein quality and yield, it can be concluded that high yield will produce a high protein and have better microstructure quality. The quality of gelatin morphology is closely related to the quality of viscosity (Nuamsrinuan et al, 2015) The quality of gelatin morphology is also influenced by cross-linking that occurs in gelatin (Gaspar-Pintiliescu et al, 2019).…”