Physicochemical characterization of biomaterials commonly used in dentistry as bone substitutes—Comparison with human bone

Figueiredo, Margarida; Henriques, José; Martins, Gabriela Botelho; Guerra, Fernando; Judas, F; Figueiredo, Helena

doi:10.1002/jbm.b.31529

Cited by 130 publications

(146 citation statements)

References 47 publications

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“…It has been shown that physico-chemical properties (like chemical composition and particle morphology) are among the most important factors that influence the material performance in vivo, causing significantly different biological responses [22,[24][25][26][27]. In fact, such properties may change the behavior of macrophages in processes like adhesion, apoptosis, fusion and cytokine secretion [28].…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Comparison of a xenogeneic and an alloplastic material used in dental implants in terms of physico-chemical characteristics and in vivo inflammatory response

Figueiredo

Coimbra

Cabrita

et al. 2013

Materials Science and Engineering: C

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Two commercial bone grafts used in dentistry (Osteobiol Gen-Os®, a xenograft of porcine origin, and Bonelike®, a hydroxyapatite based synthetic material), in the form of granules, were characterized and evaluated in vivo regarding the intensity of the tissue inflammatory response. These biomaterials were characterized in terms of morphology, particle size distribution, porosity and pore size, specific surface are and density. The chemical composition and structure of the materials were accessed by Fourier-Transform Infrared Spectroscopy (FTIR) and X-ray Diffraction (XRD). The graft materials were implanted in the gluteus maximus muscles of Wistar rats and the inflammatory response evaluated through histological analysis, after one week of implantation.The results showed that the two grafts have quite different characteristics in practically all the evaluated properties. While Osteobiol® exhibits a structure and composition similar to the natural bone, Bonelike® is constituted by a main crystalline phase of hydroxyapatite and two secondary phases of -and -tricalcium phosphate. Osteobiol® granules, besides being larger, are irregular, and exhibit sharp-edged tips, while those of Bonelike® are approximately cylindrical, with round contours, and more uniform in size. The in vivo response evaluated from the inflammatory infiltrates revealed that although both implants did not cause severe inflammation, Bonelike® granules elicit a consistently more intense inflammatory reaction than that triggered by the granules of Osteobiol®, particularly in terms of collagen production and formation of fibrous capsule. This reaction was partly explained in terms of the characteristics evaluated for the granules of this material.

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Section: Accepted M Manuscriptmentioning

confidence: 99%

Comparison of a xenogeneic and an alloplastic material used in dental implants in terms of physico-chemical characteristics and in vivo inflammatory response

Figueiredo

Coimbra

Cabrita

et al. 2013

Materials Science and Engineering: C

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“…Commercial Bio-HAp has been studied by Figuereido et al, fourteen different samples were used in dentistry, three of them based on HAp and the others based on calcium carbonate [11]. Kupiec et al studied the effect of calcination parameters on bone hydroxyapatite in artificial saliva and its biosafety.…”

Section: Introductionmentioning

confidence: 99%

Access to Optimal Calcination Temperature for Nanoparticle Synthesis from Hydroxyapatite Bovine Femur Bone Waste

Hammood¹,

Hassan²,

Alkhafagy³

2017

Nano BioMed ENG

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Hydroxyapatite (HAp) is one of the vital and bioactive materials that are commonly used in biomedical field and concentrated in clinical area. It is a bio-ceramic powder synthesized by using different bio-waste materials such as bovine femur bone. In this study, the bovine femur bone powder was prepared to obtain nano powder. The purpose of this study was to reach the optimal temperature to obtain nanoparticles HAp. The resulted powder was calcinated in a furnace at different temperatures (900, 950, 1000, 1050 and 1100 °C) for 2 h at the heating rate of 10 °C/min and cooled slowly in a furnace. Results showed, the formation of pure HAp by the presence of peaks corresponding to (PO 4 ) 3-at 632/cm and OH -at 3,572/cm in fourier transformation infrared spectroscopy (FTIR). For the calcined samples, there were three main peaks of 211, 112 and 300 planes at 2θ near 31.8, 32.2 and 32.9 respectively. The amorphous raw bones were transformed into crystalline phase and the lattice parameters for HAp c and a were calculated in X-ray diffraction (XRD), Raman analysis showed that the calcination process removed the organic compound from the bovine femur bones matrix. Scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX) showed that the shape of the granules was irregular, containing quantities of oxygen, magnesium, sodium and carbon; the ratio of calcium to phosphate was calculated, Atomic force microscopy (AFM) showed that the particles sizes ranged from nanometers to microns. EDX result found that the calcium to phosphate ratio reached 1.7058 after calcination at 950 °C which was close to stoichiometric hydroxyapatite (1.67). This result implied the formation of pure HAp phase at 950 °C.

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“…hydroxyapatite (HA) are well documented. The in vivo performance of particular brands of alloplastic grafts is often variable [3,8].…”

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confidence: 99%

“…On the contrary, those with greater Ca/P ratios degrade gradually or not at all and may give rise to remnants that can induce inflammation [2,8]. In addition, lower degradation rates usually result in less new bone formation around the scaffold.…”

mentioning

confidence: 99%

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Cytotoxicity Evaluation and Crystallochemical Analysis of a Novel and Commercially Available Bone Substitute Material

Bojar

Ciach²,

Kucharska³

et al. 2015

Adv Clin Exp Med

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Background. Alloplastic biomaterials are an alternative for autologous transplants and xenografts in oral surgery and dental implantology. These non-immunogenic and resorbable materials are becoming the basis for complete and predictable guided bone regeneration in many cases. The chemical composition of a great majority of them is based on calcium phosphate salts. In vivo performance is often variable. Objectives. The objective was to evaluate the biological and chemical properties of an experimental bone substitute material. Material and Methods. The present research focuses on the cytotoxicity comparison and physiochemical characterization of two biomaterials: a novel chitosan/tricalcium phosphate/alginate composite (CH/TCP/Ag) and a commercially available synthetic bone graft made of HA (60%) and βTCP (40%) (HA/TCP). The materials were evaluated according to PN-EN ISO 10993 Biological evaluation of medical devices i.e. cytotoxicity on mouse fibroblasts (L929) and, in addition, tests on human osteoblasts (hFOB1.19) and human osteosarcoma (MG-63) were conducted. The crystallochemical analysis was performed using the X-ray powder diffraction method. The Bruker--AXS D8 Advance diffractometer (Karlsruhe, Germany) was used to collect diffractograms. Results. The tested materials showed a close resemblance in chemical composition and a considerable differentiation in cytotoxic response. Conclusions.The novel composite demonstrated a high degree of cytocompatibility, which is promising in future clinical trials (Adv Clin Exp Med 2015, 24, 3, 511-516).

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Physicochemical characterization of biomaterials commonly used in dentistry as bone substitutes—Comparison with human bone

Cited by 130 publications

References 47 publications

Comparison of a xenogeneic and an alloplastic material used in dental implants in terms of physico-chemical characteristics and in vivo inflammatory response

Comparison of a xenogeneic and an alloplastic material used in dental implants in terms of physico-chemical characteristics and in vivo inflammatory response

Access to Optimal Calcination Temperature for Nanoparticle Synthesis from Hydroxyapatite Bovine Femur Bone Waste

Cytotoxicity Evaluation and Crystallochemical Analysis of a Novel and Commercially Available Bone Substitute Material

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