Physiological and biochemical responses of the green algaPachycladella chodatii(SAG 2087) to sodicity stress

Abstract

The effects of various concentrations of different carbon sources (Na 2 CO 3 and NaHCO 3) as sodicity stress on growth parameters, CO 2 consumption rate, enzyme activity, intracellular lipid content, and fatty acid profiles of Pachycladella chodatii were studied. Generally, the total chlorophyll was increased by increasing the concentrations of Na 2 CO 3 and NaHCO 3. The biomass productivity as well consumption rate of carbon dioxide of P. chodatii reached the highest values with increasing concentrations of Na 2 CO 3 and NaHCO 3. The soluble protein content of P. chodatii was highest at the lowest Na 2 CO 3 and NaHCO 3 concentrations. The addition of different concentrations of Na 2 CO 3 and NaHCO 3 in the growth media induces lipoxygenase and superoxide dismutase specific activity. Catalase and total antioxidant enzymes were increased by supplementing the growth media with 60 and 45 mg l À1 of Na 2 CO 3 and NaHCO 3 , respectively. Hydrogenase uptake activity in P. chodatii increased gradually in all treated cultures with the time elapsed recording the maximum activity after 11 days of growth especially at 60, 45 mg l À1 of Na 2 CO 3 and NaHCO 3 respectively. Lipids content was increased at low concentration of Na 2 CO 3 (40 and 15 mg l À1) and NaHCO 3 (60, 45 mg l À1) respectively. Subsequent to algal cultivation in different concentrations of Na 2 CO 3 , the cultures were filtered and biodiesel was prepared by direct esterification of dry algal biomass. Methyl esters of palmitic, elaidic and stearic acids represented the major components while myristic, pentadecanoic and 9,12-octadecenoic acids represented a minor component of biodiesel produced from P. chodatii treated with different concentrations of Na 2 CO 3 and NaHCO 3 .

Bicarbonate supplementation enhances growth and biochemical composition of Dunaliella salina V-101 by reducing oxidative stress induced during macronutrient deficit conditions

“…These results indicate that nitrate and phosphate are essential for growth and homeostasis in the microalga, D. salina . Similarly, several reports also revealed the increase in growth and biomass of microalgae and diatoms cultured in bicarbonate amended medium 8 , 15 , 16 , 24 .…”

“…In our results, addition of bicarbonate was found to increase the antioxidant enzymes activities, which was quite consistent with results of Peng et al . 16 and Fawzy et al . 16 .…”

“…To mitigate and repair damage initiated by ROS during abiotic stress, algae have developed a defense mechanism, using a complex antioxidant systems 11 – 14 . However, in a few microalgal species, it has been reported that the ROS scavenging antioxidant enzyme in microalgae is influenced by bicarbonate 15 , 16 . To our knowledge, this is the first report studying the effect of bicarbonate amendment on antioxidant response of D. salina during nutrient deficit conditions.…”

Effect of Exogenously Applied Jasmonic Acid and Kinetin on Drought Tolerance of Wheat Cultivars Based on Morpho-Physiological Evaluation

“…Superoxide dismutase (SOD) and catalase (CAT) activities were mentioned by Fawzy et al (2017). The activity of SOD (EC 1.15.1.1) was measured in a final volume of 2 ml of a reaction medium containing 50 mM of sodium carbonate buffer (pH 10.2), 0.1 mM EDTA, 100 μl enzymatic extract, and 100 μl of 5.5 mg/ml epinephrine (dissolved in 10 mM HCl, pH 2).…”

Structure and Biochemical Analysis of Phytoplankton in the Wadi El-Rayan Lakes, El-Fayoum, Egypt.

“…RESULTS AND DISCUSSION: There was a large variation in the biochemical content of phytoplankton among species and locations across the seasons. Generally, total protein was the major biochemical constituent of the phytoplankton community; the protein content of algae is also an important criterion for their use as food (Fawzy et al, 2017). In contrast, the total lipid content was the minor constituent of the biochemical composition of the communities.…”