Physiological and developmental problems encountered by in vitro cultured plants

Bairu, Michael W.; Kane, Michael E.

doi:10.1007/s10725-011-9565-2

Cited by 57 publications

(31 citation statements)

References 14 publications

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“…Regenerants were morphologically separate from, but genetically similar to, the normal seedlings. This seems to support the view that morphological differences observed in this study may have arisen from epigenetic or physiological factors as described by Miguel and Marum (2011) and Bairu and Kane (2011).…”

Section: Cluster Analysissupporting

confidence: 91%

“…There were very large overlaps of the 95% confidence ellipses of the regenerants and wild type seedlings (Figure 2c). Lack of clear separation of the regenerants and normal seedlings based on RAPD-genic variation characters could suggest that the morphological differences observed in this study may be epigenetic or physiological as suggested by Miguel and Marum (2011) and Bairu and Kane (2011).…”

Section: Principal Coordinates (Pcoorda) Analysismentioning

confidence: 72%

“…Some of the factors responsible for somaclonal variation include plant growth regulators, physiological and biochemical stresses imposed by the in vitro culture conditions, type of explant and mode of regeneration (Neelakanda and Wang, 2012). Tissue culture-induced somaclonal variation can produce off-types which are undesirable when the expected outcome is mass production of uniform genotypes (Bairu and Kane, 2011). However, it can also be a source of useful novel variations (Larkin and Scowcroft, 1981;Tang, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Mulanda¹,

Chuhila²,

Awori³

et al. 2015

Afr. J. Biotechnol.

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Somaclonal variation induced in vitro during tissue culture can be a problem in clonal micropropagation of elite plants. This study investigated the extent of morphological and genetic similarity or dissimilarity between Melia volkensii in vitro plants (somatic seedlings) obtained via somatic embryogenesis and normal seedlings. Comparisons were made between in vitro plants regenerated directly from cotyledon explants, indirectly from zygotic embryos and normal seedlings of the same parent trees. Regeneration was achieved using half MS medium supplemented with 0.05 mg/l thidiazuron. Shoots were elongated in half MS with 0.1 mg/l BAP plus 0.01 mg/l IAA then rooted in half MS with 0.1 mg/l IBA and 0.1 mg/l NAA. Six morphometric and five meristic characters were used for the morphological characterization. PCR-RAPD markers were used for assessment of genetic similarity or distance. Multivariate analysis using principal coordinates, cluster analysis, analysis of similarities (Anosim) and similarity percentages analysis (SIMPER) revealed significant dissimilarities (p< 0.0001) in morphometric and meristic characters between the in vitro plants and normal seedlings. However, significant similarity (p<0.01) was observed in the RAPD-genic characters of the in vitro plants and normal seedlings. Out of six morphometric traits, taproot length, internode length and shoot height were the most important sources of dissimilarity, cumulatively accounting for 72.37% of overall morphometric dissimilarity. Number of lateral roots was the single most important source of meristic dissimilarity, with 77.02% contribution. Plants regenerated directly from cotyledons were more similar to the normal seedlings in morphological and RAPD-marker characters than those regenerated indirectly from zygotic embryos. This study paves the way for identification of trait-specific RAPD markers for further characterization through sequence-characterized amplified regions (SCARs).

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Section: Cluster Analysissupporting

confidence: 91%

Section: Principal Coordinates (Pcoorda) Analysismentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

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Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Mulanda¹,

Chuhila²,

Awori³

et al. 2015

Afr. J. Biotechnol.

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“…in vitro, sofrem uma situação de estresse e crescem sob condições bem diferentes das naturais (Molinier et al, 2006;Van Staden et al, 2006;Desjardins et al, 2009). Esse tipo de alteração, ao contrário da variação somaclonal, é temporária, o que torna possível a reversão após algum tempo (Bairu & Kane, 2011). A cultivar Woodard foi a que apresentou maiores diâmetros, sem que tenha diferido significativamente Briteblue (Tabela 1).…”

Section: Resultsunclassified

Desempenho de mudas de mirtilo obtidas por micropropagação ou estaquia

Souza

Schuch

Antunes

et al. 2011

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar o desenvolvimento vegetativo e o início do período produtivo de mirtilos (Vaccinium virgatum) propagados por estaquia e micropropagação. No ano de 2009, foi implantado um pomar de mirtilo com mudas de um ano de idade, em espaçamento de 1,3x4,0 m. Utilizaram-se as cultivares Bluegem, Briteblue e Woodard, do grupo "rabbiteye". Foram avaliados os seguintes parâmetros: altura de planta, diâmetro e número de brotações, diâmetro médio dos frutos, frutos colhidos por planta, produção média por planta, produtividade estimada por hectare, massa de matéria fresca por fruto, teor de sólidos solúveis, acidez titulável e pH. Plantas obtidas pela técnica de micropropagação apresentaram maior crescimento vegetativo inicial, em razão do rejuvenescimento causado por este tipo de propagação. O método de propagação não influenciou a qualidade dos frutos. Ao contrário do esperado, as plantas micropropagadas não mostraram atraso no início da produção de frutos e apresentaram produtividade e qualidade de frutos semelhantes às de plantas propagadas por estaquia.Termos para indexação: Vaccinium virgatum, juvenilidade, produção de mudas, propagação vegetativa, rejuvenescimento. Performance of blueberry seedlings obtained by micropropagation or cuttingAbstract -The objective of this work was to evaluate the vegetative development and the onset of the productive period of blueberry (Vaccinium virgatum) seedlings obtained by semi-hardwood cuttings or micropropagation. In 2009, a blueberry orchard was established with one-year-old seedlings, at 1.3x4.0 m spacing. The cultivars Bluegem, Briteblue, and Woodard, from the rabbiteye group were utilized. The following parameters were evaluated: plant height, number and diameter of shoot, berry average diameter, number of harvested fruit per plant, average production per tree, estimated yield by hectare, fresh matter per fruit, content of soluble solids, titratable acidity and pH. Plants originated from the micropropagation technique showed high rate of initial vegetative growth, due to the rejuvenation caused by this sort of propagation. Propagation methods did not affect fruit quality. Despite the expected, micropropagated plants showed no delay in the initial fruit production and its fruit yield and quality were similar to those of cutting-propagated plants.

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“…Furthermore, any complex organorgan and plant-environment interaction can be controlled or removed, and the level of stress can be accurately and conveniently controlled [60]. All this together makes that some aspects of plant growth, that were barely understood before the advancement of the science of tissue culture, such as the metabolism and interaction of plant hormones, as well as their physiological effects can be deeply studied [61].…”

Section: In Vitro Tissue Culture As a Tool For Physiological And Biocmentioning

confidence: 99%

In vitro Tissue Culture, a Tool for the Study and Breeding of Plants Subjected to Abiotic Stress Conditions

Pérez-Clemente¹,

Gómez-Cádenas²

2012

Recent Advances in Plant in Vitro Culture

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Physiological and developmental problems encountered by in vitro cultured plants

Cited by 57 publications

References 14 publications

Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Morphological and RAPD-marker characterization of Melia volkensii (Grke) in vitro plants regenerated via direct and indirect somatic embryogenesis

Desempenho de mudas de mirtilo obtidas por micropropagação ou estaquia

In vitro Tissue Culture, a Tool for the Study and Breeding of Plants Subjected to Abiotic Stress Conditions

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