Physiological and Phytochemical Responses of Rosemary (Rosmarinus officinalis L.) Plant on  in vitro Callus Formation

El-Zefzafy, M. M.; Dawoud, Gouda T. M.; Shahhat, Ismail

doi:10.9734/ejmp/2016/30486

Cited by 2 publications

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“…Besides, in vitro culture conditions with optimal supplement could stimulate the secondary metabolites, which higher than the intact plant. For example, callus culture of Rosmarinus officinalis (El‐Zefzafy et al., 2016), Salvia miltiorrhiza (Wu et al., 2016), and Ocimum basilicum (Nazir et al., 2020). Another strategy for enhancing secondary metabolite in callus, such as cell suspension culture, elicitation with a suitable supplement or elicitor, and stress condition culture, should be applied for further study.…”

Section: Resultsmentioning

confidence: 99%

Section: Determination Of Phenolic Acid and Flavonoid Contents In T Laurifolia Leaves And Callus Culture Of T Laurifoliamentioning

confidence: 99%

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Comparative study of callus culture and leaves of Thunbergia laurifolia for their bioactive constituents and the activation of AMPK and GLUT‐dependent glucose uptake on rat skeletal muscle (L6) cells

Choonong

Jabsanthia

Waewaram

et al. 2021

J Food Process Preserv

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Diabetes mellitus (DM) is a group of metabolic diseases characterized by chronic hyperglycemia due to defects in the mechanism for insulin secretion. The low insulin levels to achieve adequate response and insulin resistance of target tissues and effector enzymes or genes are responsible for these metabolic abnormalities (Kharroubi & Darwish, 2015;Raffel & Goodarzi, 2014). The glucose uptake in muscle cells could process by various mechanism pathways.The common mechanism, such as AMP-activated protein kinase (AMPK) and insulin-regulated glucose transporter (GLUT), plays an essential role in regulates cellular metabolism (Musi et al., 2001). AMPK-α1 is commonly distributed in various cells, and AMPK-α2 is available expressed in the heart, liver, and skeletal muscle (Long & Zierath, 2006). Glucose transporters transfer glucose from the extracellular space (derived from the bloodstream) through cells.The glucose reduced in the blood results of insulin action. Via the GLUT2, glucose joins the β-cells in the pancreas, and this is a significant trigger that contributes to the release of insulin that enters the bloodstream (Stein & Litman, 2015).

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Section: Resultsmentioning

confidence: 99%

Section: Determination Of Phenolic Acid and Flavonoid Contents In T Laurifolia Leaves And Callus Culture Of T Laurifoliamentioning

confidence: 99%

Comparative study of callus culture and leaves of Thunbergia laurifolia for their bioactive constituents and the activation of AMPK and GLUT‐dependent glucose uptake on rat skeletal muscle (L6) cells

Choonong

Jabsanthia

Waewaram

et al. 2021

J Food Process Preserv

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Sustainable Exploitation of Greek Rosmarinus officinalis L. Populations for Ornamental Use through Propagation by Shoot Cuttings and In Vitro Cultures

et al. 2022

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Rosmarinus officinalis L. belongs to the Lamiaceae family and is cultivated worldwide due to its diverse uses as an ornamental evergreen shrub in gardening, as well as a food seasoning and a natural medicine. The present research was conducted to study the morphological traits of seven wild-grown rosemary populations in Greece, as well as the propagation of two selected populations with the most desirable ornamental characteristics, by the use of shoot cuttings and in vitro cultures. From the study of the morphological traits of the seven populations, those with interesting features were grown in the areas of Amaliada and Piperia and, thus, were chosen for studying their propagation. Rooting of the shoot cuttings was carried out during the four seasons of the year, using potassium salt Indole-3-butyric acid (K-IBA) at concentrations of 0.5 and 1 g·L−1, in various substrates under the intermittent mist or fog system. It was found that the shoot cuttings rooted easily in all four seasons, but there were better results for the population of ‘Piperia’ in autumn, with 1 g·L−1 K-IBA (80%), and ‘Amaliada’ in spring, with 0.5 g·L−1 K-IBA (82.5%), while higher rooting percentages were achieved in the fog system, on a substrate consisting of perlite and peat, in a ratio of 2:1 (85%). For the in vitro cultures, shoot tips excised from the two selected populations were successfully disinfested by pre-soaking in an antioxidant solution and then, by sterilizing them in 0.6% (w/v) NaOCl, followed by transferring them onto a Murashige and Skoog (MS) nutrient medium. ‘Amaliada’ cultures proved to be the most productive population (2.1 shoots per explant), with the highest shoot formation frequency (91.6%), when cultivated on the MS nutrient medium without plant growth regulators. For ‘Piperia’ cultures, the highest shoot formation frequency (66.6%) was achieved on the MS nutrient medium supplemented with 0.25 or 0.5 mg·L−1 6-Benzyloaminopurine (BAP) and 0.1 mg·L−1 Indole-3-acetic acid (IAA). Spontaneous root formation frequency was noticed on the MS nutrient medium, containing 0.5 mg·L−1 BAP and 0.1 mg·L−1 IAA, for both ‘Amaliada’ and ‘Piperia’ cultures (50% and 41.6%, respectively) in a single stage, with root lengths of 7.1 and 5.3 cm, respectively. Rosemary plantlets, with roots formed in vitro after transplanting them in soilless substrate, were acclimatized adequately in the greenhouse environment (~70%).

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Physiological and Phytochemical Responses of Rosemary (Rosmarinus officinalis L.) Plant on in vitro Callus Formation

Cited by 2 publications

References 0 publications

Comparative study of callus culture and leaves of Thunbergia laurifolia for their bioactive constituents and the activation of AMPK and GLUT‐dependent glucose uptake on rat skeletal muscle (L6) cells

Comparative study of callus culture and leaves of Thunbergia laurifolia for their bioactive constituents and the activation of AMPK and GLUT‐dependent glucose uptake on rat skeletal muscle (L6) cells

Sustainable Exploitation of Greek Rosmarinus officinalis L. Populations for Ornamental Use through Propagation by Shoot Cuttings and In Vitro Cultures

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