Physiological and transcriptional profiling of surfactin exerted antifungal effect against Candida albicans

Jakab, Ágnes; Kovács, Fruzsina; Balla, Noémi; Tóth, Zoltán; Ragyák, Ágota; Sajtos, Zsófi; Csillag, Kinga; Nagy-Köteles, Csaba; Nemes, Dániel; Bácskay, Ildikó; Pócsi, István; Majoros, László; Kovács, Ákos T.; Kovács, Renátó

doi:10.1016/j.biopha.2022.113220

Cited by 12 publications

(7 citation statements)

References 56 publications

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“…[55], Brachyspira hyodysenteriae, Clostridium perfringens [56], Xanthomonas oryzae [57], Propionibacterium acnes [58]. A few surfactins have been reported to possess antifungal activities against F. oxysporum [59], Fusarium moniliforme [60, 61], Candida albicans [62], Magnaporthe grisea [49], Fusarium solani , Trichoderma atroviride , and Trichoderma reesei [55]. Additionally, the surfactin has synergistic inhibition effects on fungi by combining with other antifungal agents [60].…”

Section: Discussionmentioning

confidence: 99%

Antifungal activities of Bacillus velezensis FJAT‐52631 and its lipopeptides against anthracnose pathogen Colletotrichum acutatum

et al. 2023

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This study was aim at investigating antifungal activities of Bacillus velezensis FJAT‐52631 and its lipopeptides against Colletotrichum acutatum ex situ and in situ. The results showed that the strain FJAT‐52631 and its crude lipopeptides (10 mg/ml) exhibited strong inhibitory effects on growth of C. acutatum FJAT‐30256 with an inhibition rate of 75.3% and an inhibition zone diameter of 17.66 mm, respectively. Both the viable bacterial cultures and lipopeptides of FJAT‐52631 could delay the onset of loquat anthracnose by 1 day and lower the incidence of loquat anthracnose in situ. The whole cultures of B. velezensis FJAT‐52631 displayed a 50% biocontrol efficacy on loquat anthracnose at the fourth day after inoculation, but the crude lipopeptides not. The average lesion diameter of the whole‐culture treated group was 5.62 mm, which was smaller than that of control group (6.81 mm). All the three types of lipopeptides including iturin A, fengycin, and surfactin A secreted from the strain FJAT‐52631 exhibited antifungal activities. Among them, surfactin A displayed higher antifungal activity at a concentration of 1.25 mg/mL than other two lipopeptides even if at a concentration of 60 mg/mL. Thus, the results indicated that surfactin A produced by FJAT‐52631 played a major role in the biocontrol of the loquat anthracnose. Scanning electron microscopy (SEM) observation revealed the structural deformities in the mycelia of C. acutatum. The above results suggested that the antifungal lipopeptides from B. velezensis FJAT‐52631 would be potential in biocontrol against anthracnose disease of loquat caused by C. acutatum.

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Section: Discussionmentioning

confidence: 99%

Antifungal activities of Bacillus velezensis FJAT‐52631 and its lipopeptides against anthracnose pathogen Colletotrichum acutatum

et al. 2023

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“…Surfactin has been mainly reported for its resistance to bacteria and can significantly enhance other antifungal effects. However, in recent years, some reports have shown that surfactins also have antifungal activity against Fusarium oxysporum , F. moniliforme , F. solani [ 32 ], Candida albicans [ 33 ] and so on. The expression of Fen gene was also increased in induced culture, which was 1.76 times higher than pure culture.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of the production enhancement mechanism of antimicrobial lipopeptides of Streptomyces bikiniensis HD-087 by co-culture with Magnaporthe oryzae Guy11

et al. 2022

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The lipopeptides produced by Streptomyces bikiniensis have a significant inhibitory effect on Magnaporthe oryzae, but the low yield limits its application. In this study, the anti-M. oryzae activity of the broth of S. bikiniensis HD-087 co-cultured with M. oryzae Guy11 mycelium has risen by 41.22% compared with pure culture, and under induction conditions of adding Guy11-inducer (cell-free supernatant of M. oryzae Guy11), the activity of strain HD-087 improved 61.76%. The result proved that the enhancement effect of Guy11 on the antimicrobial activity of HD-087 was mainly related to metabolites but mycelium cells. Under optimum induction conditions, NRPS gene expression levels of HD-087 were significantly increased by induction with Guy11-inducer, the biomass of HD-087 had no significant change, but crude extract of lipopeptide (CEL) production was 107.4% higher than pure culture, and TLC result under acid hydrolysis showed that the induced culture has one component more than pure culture. To clarify the regulation mechanism of improving lipopeptide production of HD-087 with Guy11-inducer, transcriptomic analysis was performed using RNAseq to compare the induced culture and pure culture. In the induced culture, 943 genes were up-regulated, while 590 genes were down-regulated in DEGs (differentially expressed genes). KEGG results showed that the expression of genes related to amino acid synthesis, fatty acid metabolism, TCA cycle and pyruvate metabolism pathway were significantly increased. The increased expression of genes related to these metabolic pathways provided sufficient precursors for lipopeptide synthesis. Accordingly, key enzyme genes responsible for the synthesis of lipopeptides Srf and NRPS was significantly increased. Quorum sensing related genes OppA and MppA were significantly up-regulated, and then ComP was activated and promoted lipopeptide synthesis. These results provided a scientific basis for using M. oryzae to induce the increase of the production of Streptomyces lipopeptides, and also laid a foundation for further exploring the co-culture mechanisms among different genera.

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“…For example, surfactin caused a significant reduction in the intracellular content of iron, manganese and zinc in Candida albicans compared to the cells of the control treatment. In addition, the expression of genes involved in the synthesis of ergosterol decreased, thus affecting the growth of the fungus [39]. intracellular content of iron, manganese and zinc in Candida albicans compared to the cells of the control treatment.…”

Section: Esi-ms/ms Analysis Of Surfactinmentioning

confidence: 98%

Identification and Quantification of Lipopeptide Homologues Induced and Produced by Bacillus amyloliquefaciens

Ley-López,

Heredia,

Martín-Hernández

et al. 2023

Fermentation

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Cyclic lipopeptides (LPs) are potentially promising in the agricultural, industrial and pharmaceutical sectors. LPs have a variable hydrophilic cyclic peptide part attached to a variable fatty acid chain. One limitation of these compounds is their low availability due to their limited production by bacteria. The objective of this study was to identify and quantify homologues of LPs biosynthesized by Bacillus amyloliquefaciens using ultra-performance liquid chromatography (UPLC–MS/MS) after inducing the synthesis of these secondary metabolites using different inducers, including chemical compounds and inactive cells of Colletotrichum sp. Four homologues were identified in the iturin family (bacillomycin D), and the iturin homologue with the highest synthesis was the molecular ion m/z 1031.54, with 173.1 µg mg−1 crude extract. In addition, seven homologues were detected in the fengycin family (four of fengycin A and three of fengycin B), and the homologue with the highest content was the molecular ion m/z 1463.69 (fengycin A), with 3288 ± 528.5 ng mg−1 crude extract. Finally, five homologues were identified in the surfactin family, where the highest concentration was observed for the molecular ion m/z 1036.68, with 61.5 ± 3.01 µg mg−1 crude extract. The highest concentration of LP homologues (iturin, fengycin and surfactin) synthesized by B. amyloliquefaciens was detected in the presence of inactive cells of Coletotrichum sp., suggesting that the inducing substance is associated with the inducer’s cell envelope and could be a single protein or a structure that includes protein components.

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Physiological and transcriptional profiling of surfactin exerted antifungal effect against Candida albicans

Cited by 12 publications

References 56 publications

Antifungal activities of Bacillus velezensis FJAT‐52631 and its lipopeptides against anthracnose pathogen Colletotrichum acutatum

Antifungal activities of Bacillus velezensis FJAT‐52631 and its lipopeptides against anthracnose pathogen Colletotrichum acutatum

Transcriptome analysis of the production enhancement mechanism of antimicrobial lipopeptides of Streptomyces bikiniensis HD-087 by co-culture with Magnaporthe oryzae Guy11

Identification and Quantification of Lipopeptide Homologues Induced and Produced by Bacillus amyloliquefaciens

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