Physiological, biochemical and proteomic insight into the response system of Streptomyces hygroscopicus OsiSh-2 to rice blast fungus toxins

Liu, Ying; Chen, Ning; Gao, Yan; Bu, Zhigang; Niu, Shuqi; Wang, Yirong; Liu, Xuanming; Zhu, Yonghua

doi:10.1016/j.apsoil.2021.104058

Cited by 4 publications

(3 citation statements)

References 65 publications

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“…The collected fractions were then concentrated by lyophilization. Then, select protein samples with high and no HR activity were analyzed by nano-liquid chromatography-tandem mass spectrometry (NanoLC-MS/MS) as described previously ( 75 ).…”

Section: Methodsmentioning

confidence: 99%

The Rice Endophyte-Derived α-Mannosidase ShAM1 Degrades Host Cell Walls To Activate DAMP-Triggered Immunity against Disease

Liu

et al. 2023

Microbiol Spectr

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Section: Methodsmentioning

confidence: 99%

The Rice Endophyte-Derived α-Mannosidase ShAM1 Degrades Host Cell Walls To Activate DAMP-Triggered Immunity against Disease

Liu

et al. 2023

Microbiol Spectr

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“…As for the inhibitory effect on the development of conidia of 70-15, the assay was based on the method in the previous study and replaced the cellfree culture filtrate (CFC) of OsiSh-2 with 5 mM thiamine. 37 To investigate the antagonism of WT and ΔTHIC against M. oryzae, the dual-culture assay was performed. 28 M. oryzae 70-15 was inoculated in the center of the PDA plate, and the two strains were one-line streaked 30 mm above and below the center.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…After incubating at 28 °C for 10 days, the growth of 70-15 mycelia was photographed. As for the inhibitory effect on the development of conidia of 70-15, the assay was based on the method in the previous study and replaced the cell-free culture filtrate (CFC) of OsiSh-2 with 5 mM thiamine …”

Section: Methodsmentioning

confidence: 99%

The Vital Role of ShTHIC from the Endophyte OsiSh-2 in Thiamine Biosynthesis and Blast Resistance in the OsiSh-2-Rice Symbiont

Liu

Qin

Chen

et al. 2022

J. Agric. Food Chem.

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Endophytes can benefit the growth and stress resistance of host plants by secreting bioactive components. Thiamine is an essential vitamin involved in many metabolic pathways and can only be synthesized by microbes and plants. In this study, we found that thiamine could inhibit the development of the phytopathogen Magnaporthe oryzae and decrease the rice blast index under field conditions. In the thiamine biosynthesis pathway, the key enzyme ShTHIC of an endophyte Streptomyces hygroscopicus OsiSh-2 and OsTHIC of rice (Oryza sativa) were highly homologous. Gene overexpression or knockout approaches revealed that both THIC contributed to thiamine synthesis and resistance to M. oryzae. Furthermore, S. hygroscopicus OsiSh-2 colonization led to a decrease in the thiamine synthesis level of rice but still maintained thiamine homeostasis in rice. However, inoculation with the ShTHIC knockout strain ΔTHIC reduced the thiamine content in rice, although the thiamine synthesis level of rice was increased. After infection with M. oryzae, blast resistance was dramatically improved in OsiSh-2-inoculated rice but decreased in ΔTHIC-inoculated rice compared with non-inoculated rice. This result demonstrated that ShTHIC could regulate thiamine biosynthesis and consequently assist blast resistance in the OsiSh-2-rice symbiont. Our results revealed a novel blast-resistance mechanism mediated by a key thiamine biosynthetic enzyme from an endophyte OsiSh-2.

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Antifungal Metabolites of Streptomyces chrestomyceticus STR-2 Inhibits Magnaporthe oryzae, the Incitant of Rice Blast

et al. 2023

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Physiological, biochemical and proteomic insight into the response system of Streptomyces hygroscopicus OsiSh-2 to rice blast fungus toxins

Cited by 4 publications

References 65 publications

The Rice Endophyte-Derived α-Mannosidase ShAM1 Degrades Host Cell Walls To Activate DAMP-Triggered Immunity against Disease

The Rice Endophyte-Derived α-Mannosidase ShAM1 Degrades Host Cell Walls To Activate DAMP-Triggered Immunity against Disease

The Vital Role of ShTHIC from the Endophyte OsiSh-2 in Thiamine Biosynthesis and Blast Resistance in the OsiSh-2-Rice Symbiont

Antifungal Metabolites of Streptomyces chrestomyceticus STR-2 Inhibits Magnaporthe oryzae, the Incitant of Rice Blast

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