Physiological comparisons among Spathaspora passalidarum, Spathaspora arborariae, and Scheffersomyces stipitis reveal the bottlenecks for their use in the production of second-generation ethanol

Campos, Valquíria Júnia; Ribeiro, Lílian Emídio; Albuini, Fernanda Matias; Castro, Alex Gazolla de; Fontes, Patrícia Pereira; Silveira, Wendel Batista da; Rosa, Carlos A.; Fietto, Luciano Gomes

doi:10.1007/s42770-022-00693-6

Cited by 10 publications

(8 citation statements)

References 57 publications

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“…Whereas, at 0.2% v/v, the lag phase of the strain was extended, like in the furfural treatments. Cell growth delay and extension of the lag phase in S. stipitis has been reported when furfural or acetic acid were used as inhibitors (Ma et al, 2013;Zhu et al, 2018;Campos et al, 2022). Interestingly, when the bc1 complex ofS.…”

Section: Resultsmentioning

confidence: 99%

Influence of furfural and acetic acid on bioenergetics of Scheffersomyces stipitis

Granados-Arvizu,

Saucedo-Gutierrez,

Escamilla-García

et al. 2023

Preprint

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Lignocellulosic material is the most prominent carbon source to obtain profitable biotechnological processes, but compounds like furfural and acetic acid are highly toxic to yeasts. Nonetheless, research about the molecular mechanism of furfural and acetic acid toxicity is still scarce in yeasts like Scheffersomyces stipitis. Thus, this work aimed to identify the role of furfural and acetic acid on S. stipitis in bioenergetic and fermentation parameters. Here, we provide evidence that furfural and acetic acid caused a cell growth delay and extension of the lag phase. The mitochondrial membrane potential decreased in all treatments without differences among inhibitors or concentrations. Interestingly, the reactive oxygen species increased when the inhibitors concentrations were from 0.1 to 0.3% (v/v). The glycolytic flux was not significantly ( p>0.05) altered by acetic acid, but furfural caused different effects. The ethanol production decreased significantly (4.32 g/L in furfural and 5.06 g/L in acetic acid) compared to the control (26.3 g/L). At the same time, biomass was not significantly different in almost all treatments compared to the control. This study provides additional understanding of the effects of furfural and acetic acid at mitochondrial level in a pentose-fermenting yeast like S. stipitis.

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Section: Resultsmentioning

confidence: 99%

Influence of furfural and acetic acid on bioenergetics of Scheffersomyces stipitis

Granados-Arvizu,

Saucedo-Gutierrez,

Escamilla-García

et al. 2023

Preprint

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“…This use will depend on the microorganisms with capacity to metabolise this pentose. Several yeast species stand out as capable candidates for producing many of those products [ 18 , 19 , 20 , 21 ], such as the yeast Meyerozyma caribbica [ 22 ]. This is the new epithet of Pichia caribbica and the teleomorph of Candida fermentati (Division Ascomycota, class Saccharomycetes, order Saccharomycetales, family Debaryomycetaceae—Index Fungorum).…”

Section: Introductionmentioning

confidence: 99%

Meyerozyma caribbica Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

Alencar

Freitas

Guimarães

et al. 2023

JoF

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The production of fuels and other industrial products from renewable sources has intensified the search for new substrates or for the expansion of the use of substrates already in use, as well as the search for microorganisms with different metabolic capacities. In the present work, we isolated and tested a yeast from the soil of sugarcane irrigated with vinasse, that is, with high mineral content and acidic pH. The strain of Meyerozyma caribbica URM 8365 was able to ferment glucose, but the use of xylose occurred when some oxygenation was provided. However, some fermentation of xylose to ethanol in oxygen limitation also occurs if glucose was present. This strain was able to produce ethanol from molasses substrate with 76% efficiency, showing its tolerance to possible inhibitors. High ethanol production efficiencies were also observed in acidic hydrolysates of each bagasse, sorghum, and cactus pear biomass. Mixtures of these substrates were tested and the best composition was found for the use of excess plant biomass in supplementation of primary substrates. It was also possible to verify the production of xylitol from xylose when the acetic acid concentration is reduced. Finally, the proposed metabolic model allowed calculating how much of the xylose carbon can be directed to the production of ethanol and/or xylitol in the presence of glucose. With this, it is possible to design an industrial plant that combines the production of ethanol and/or xylitol using combinations of primary substrates with hydrolysates of their biomass.

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“…Scheffersomyces stipitis is a Crabtree‐negative yeast, naturally able to consume both hexose and pentose sugars (Jeffries & Van Vleet, 2009 ), making it an interesting cell factory for the valorization of diverse residual biomasses, such as those considered in this work. Nonetheless, the ability of this yeast to valorize residual biomasses into compounds of interest is almost unexplored, as it is mainly considered as a potential cell factory for bioethanol production (Biazi et al, 2022 ; Campos et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Scheffersomyces stipitis ability to valorize different residual biomasses for vitamin B₉ production

Mastella

Senatore

Beltrani

et al. 2022

Microbial Biotechnology

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Sugar beet pulp (SBP), sugar beet molasses (SBM) and unfermented grape marcs (UGM) represent important waste in the agro‐food sector. If suitably pre‐treated, hexose and pentose sugars can be released in high quantities and can subsequently be used by appropriate cell factories as growth media and for the production of (complex) biomolecules, accomplishing the growing demand for products obtained from sustainable resources. One example is vitamin B 9 or folate, a B‐complex vitamin currently produced by chemical synthesis, almost exclusively in the oxidized form of folic acid (FA). It is therefore desirable to develop novel competitive strategies for replacing its current fossil‐based production with a sustainable bio‐based process. In this study, we assessed the production of natural folate by the yeast Scheffersomyces stipitis , investigating SBM, SBP and UGM as potential growth media. Pre‐treatment of SBM and SBP had previously been optimized in our laboratory; thus, here we focused only on UGM pre‐treatment and hydrolysis strategies for the release of fermentable sugars. Then, we optimized the growth of S. stipitis on the three media formulated from those biomasses, working on inoculum pre‐adaptation, oxygen availability and supplementation of necessary nutrients to support the microorganism. Folate production, measured with a microbiological assay, reached 188.2 ± 24.86 μg/L on SBM, 130.6 ± 1.34 μg/L on SBP and 101.9 ± 6.62 μg/L on UGM. Here, we demonstrate the flexibility of S. stipitis in utilizing different residual biomasses as growth media. Moreover, we assessed the production of folate from waste, and to the best of our knowledge, we obtained the highest production of folate from residual biomasses ever reported, providing the first indications for the future development of this microbial production process.

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Physiological comparisons among Spathaspora passalidarum, Spathaspora arborariae, and Scheffersomyces stipitis reveal the bottlenecks for their use in the production of second-generation ethanol

Cited by 10 publications

References 57 publications

Influence of furfural and acetic acid on bioenergetics of Scheffersomyces stipitis

Influence of furfural and acetic acid on bioenergetics of Scheffersomyces stipitis

Meyerozyma caribbica Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

Scheffersomyces stipitis ability to valorize different residual biomasses for vitamin B₉ production

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Physiological comparisons among Spathaspora passalidarum, Spathaspora arborariae, and Scheffersomyces stipitis reveal the bottlenecks for their use in the production of second-generation ethanol

Cited by 10 publications

References 57 publications

Influence of furfural and acetic acid on bioenergetics of Scheffersomyces stipitis

Influence of furfural and acetic acid on bioenergetics of Scheffersomyces stipitis

Meyerozyma caribbica Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

Scheffersomyces stipitis ability to valorize different residual biomasses for vitamin B9 production

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Scheffersomyces stipitis ability to valorize different residual biomasses for vitamin B₉ production