Physiological response of wheat seedlings to mild and severe osmotic stress

Kocheva, Konstantina; Kartseva, T.; Landjeva, S.; Georgiev, G. I.

doi:10.1556/crc.37.2009.2.6

Cited by 16 publications

(23 citation statements)

References 31 publications

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“…On average, no significant differences between the two types of genotypes were observed regarding the leaf thickness, and the trichome and stomata density (Table ). Figure S2 presents micrographs of leaf cross sections and leaf epidermal impressions in two genotypes—Katya, which is considered the most drought‐tolerant modern variety under the conditions of south‐eastern Europe (Kocheva et al., ), and Slomer, a historic variety of unknown tolerance to drought stress. Based on the micrographs, no distinct differences in leaf morphology were noticed between the two genotypes.…”

Section: Resultsmentioning

confidence: 99%

Relationships between leaf morpho‐anatomy, water status and cell membrane stability in leaves of wheat seedlings subjected to severe soil drought

Petrov

Petrova

Димитров

et al. 2017

J Agronomy Crop Science

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The impact of the genotype‐specific leaf morphological and anatomical characteristics on the ability of wheat plants to preserve leaf water balance and cell membranes stability under drought stress was investigated. Seedlings of six modern semi‐dwarf (carriers of Rht, Reduced height genes) and six old tall bread wheat varieties were subjected to soil drought by withholding watering for 6 days. Morpho‐anatomical traits (leaf area, perimeter, thickness, stomata and trichome density) of daily watered (control) plants were characterized by light microscopy, scanning and image analyses. The leaf water status in both control and stressed plants was determined by measuring the relative water content (RWC). The leaf cell membranes stability in stressed plants was estimated by conductometric determination of the membranes injury index. On average, the modern semi‐dwarf varieties had less leaf area and leaf perimeter, and less dissection index, a parameter characterizing the leaf shape. Under drought stress, the modern genotypes maintained better water balance evidenced by significantly higher leaf RWC and better‐preserved the cell membranes stability supported by significantly lower Injury index. The correlations between morpho‐anatomical traits in control plants and drought tolerance‐related traits showed that the higher the leaf dissection index (i.e. more oblong leaves), the greater the water loss and the leaf membrane damages after desiccation were. The effect of shape of the evaporating surface on the water loss was modelled using wet filter paper. Similar to plant leaves, the evaporation and, respectively, water loss from paper pieces of more oblong shape (i.e. higher dissection index) was more intensive. The elucidation of the impact of the leaf shape on transpiration might contribute to better understanding of the mechanisms used by plants to maintain water reserves during drought stress and could be a basis for developing of simple and fast screening methods aiding the selection of drought tolerant genotypes.

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Section: Resultsmentioning

confidence: 99%

Relationships between leaf morpho‐anatomy, water status and cell membrane stability in leaves of wheat seedlings subjected to severe soil drought

Petrov

Petrova

Димитров

et al. 2017

J Agronomy Crop Science

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“…The latter was demonstrated by the lowest values of Injury index, an indicator of possible stress‐induced impairment of membrane permeability which calculates the degree of ion leakage from damaged tissues (Kocheva et al. ). Moreover, the lowest Injury index values of droughtened Rht‐B1c plants corresponded with the highest stress‐induced proline levels.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the noted effects of drought on the antioxidant defence system, the dwarf line Rht-B1c maintained the lowest content of the lipid degradation product MDA and better preserved membrane functioning. The latter was demonstrated by the lowest values of Injury index, an indicator of possible stress-induced impairment of membrane permeability which calculates the degree of ion leakage from damaged tissues (Kocheva et al 2009). Moreover, the lowest Injury index values of droughtened Rht-B1c plants corresponded with the highest stress-induced proline levels.…”

Section: Discussionmentioning

confidence: 99%

Changes in Water Status, Membrane Stability and Antioxidant Capacity of Wheat Seedlings Carrying Different Rht‐B1 Dwarfing Alleles under Drought Stress

Kocheva

Nenova

Karceva

et al. 2013

J Agronomy Crop Science

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Water deficiency is a major constraint to wheat productivity in drought prone regions. The wheat DELLA‐encoding height‐reducing genes (Rht) are associated with significant increase in grain yield. However, the knowledge of their benefit in dry environments is insufficient. The objective of the study was to examine the effect of induced drought on leaf water content, level of oxidative stress, cell membrane stability, accumulation of osmoprotectants and activity of some antioxidant enzymes in wheat near‐isogenic lines carrying the alleles Rht‐B1b (semidwarfing) and Rht‐B1c (dwarfing) in comparison with the tall control Rht‐B1a. Six‐day‐long water deprivation was imposed at seedling stage. Plants carrying Rht‐B1c and, to a lesser extent, those carrying Rht‐B1b performed better under stress compared with Rht‐B1a in terms of more sustained membrane integrity, enhanced osmoregulation and better antioxidant defence. These differential responses could reflect pleiotropic effects of the Rht‐B1 gene associated with the accumulation of the mutant gene product, that is, altered DELLA proteins, or might be related to allelic variations at neighbouring loci carrying candidate genes for proteins with a major role in plant water regulations and stress adaptation. These findings might be of importance to breeders when introducing Rht‐B1 alleles into wheat cultivars designed to be grown in drought liable regions.

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“…Data have shown that prolonged mild stress is less harmful than short-term exposure to severe water deficit [31]. It is probable that Mv Regiment, the other winter wheat variety, which performed quite well when subjected to water deficiency at 20°C, would have been less tolerant if stressed at 22°C, like the other genotypes.…”

Section: Discussionmentioning

confidence: 99%

Drought tolerance in cereals in terms of water retention, photosynthesis and antioxidant enzyme activities

et al. 2011

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Experiments were carried out on three bread wheat varieties, one barley and one durum wheat variety grown in pots in the phytotron and subjected to water withdrawal for 7 days during grain-filling. Leaf water loss, net assimilation rate and transpiration showed marked differences, allowing the genotypes to be ranked. Although the most resistant variety had the highest activity for ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR) and glutathione-S-transferase (GST), which did not rise further in response to drought and the most susceptible variety had the lowest values, which increased to the greatest extent under drought, the level of sensitivity could not be predicted for all the genotypes from the enzyme activity values alone. The largest increases were recorded for the APX, CAT and GR activities. In most genotypes the GR activity was correlated with that of GST, CAT and APX. Changes in the enzyme activities were observed after a decline in transpiration and photosynthesis. The range of soil moisture values over which the antioxidant enzyme activity levels remained relatively unchanged was a better indication of tolerance to drought than either basic or stress-induced activity levels.

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Physiological response of wheat seedlings to mild and severe osmotic stress

Cited by 16 publications

References 31 publications

Relationships between leaf morpho‐anatomy, water status and cell membrane stability in leaves of wheat seedlings subjected to severe soil drought

Relationships between leaf morpho‐anatomy, water status and cell membrane stability in leaves of wheat seedlings subjected to severe soil drought

Changes in Water Status, Membrane Stability and Antioxidant Capacity of Wheat Seedlings Carrying Different Rht‐B1 Dwarfing Alleles under Drought Stress

Drought tolerance in cereals in terms of water retention, photosynthesis and antioxidant enzyme activities

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