Phytochemical and Antimicrobial Evaluation of Aqueous and Organic Extracts of Calotropis procera Ait Leaf and Latex

Shobowale, O.O.; Ogbulie, N.J.; Itoandon, E.E.; Oresegun, M.O.; Olatope, S.O.A.

doi:10.1016/s0189-7241(15)30059-x

Cited by 37 publications

(31 citation statements)

References 9 publications

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“…Different solvents have varying levels of solubility for different bioactive compounds. The reason for this sequence in activity may be that bioactive compounds that cause the antimicrobial activity dissolve easily in organic compared to aqueous solvents [27]. This supports earlier findings that organic leaf extracts had stronger antimicrobial activity compared to aqueous extracts [28].…”

Section: Discussionsupporting

confidence: 81%

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Effects of Medicinal Plant Extracts and Photosensitization on Aflatoxin Producing Aspergillus flavus (Raper and Fennell)

Njoki

Okoth

Wachira

2017

International Journal of Microbiology

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This study was undertaken with an aim of exploring the effectiveness of medicinal plant extracts in the control of aflatoxin production. Antifungal properties, photosensitization, and phytochemical composition of aqueous and organic extracts of fruits from Solanum aculeastrum, bark from Syzygium cordatum, and leaves from Prunus africana, Ocimum lamiifolium, Lippia kituiensis, and Spinacia oleracea were tested. Spores from four-day-old cultures of previously identified toxigenic fungi, UONV017 and UONV003, were used. Disc diffusion and broth dilution methods were used to test the antifungal activity. The spores were suspended in 2 ml of each extract separately and treated with visible light (420 nm) for varying periods. Organic extracts displayed species and concentration dependent antifungal activity. Solanum aculeastrum had the highest zones of inhibition diameters in both strains: UONV017 (mean = 18.50 ± 0.71 mm) and UONV003 (mean = 11.92 ± 0.94 mm) at 600 mg/ml. Aqueous extracts had no antifungal activity because all diameters were below 8 mm. Solanum aculeastrum had the lowest minimum inhibitory concentration at 25 mg/ml against A. flavus UONV017. All the plant extracts in combination with light reduced the viability of fungal conidia compared with the controls without light, without extracts, and without both extracts and light. Six bioactive compounds were analyzed in the plant extracts. Medicinal plant extracts in this study can control conidia viability and hence with further development can control toxigenic fungal spread.

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Section: Discussionsupporting

confidence: 81%

“…The bioactive compounds detected in this study have been shown to cause antimicrobial activities in other studies through various mechanisms [27, 36–38]. Increase in the concentration of bioactive compounds increases the antifungal activity as reported by [39].…”

Section: Discussionsupporting

confidence: 67%

Effects of Medicinal Plant Extracts and Photosensitization on Aflatoxin Producing Aspergillus flavus (Raper and Fennell)

Njoki

Okoth

Wachira

2017

International Journal of Microbiology

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“…The C. procera leaves powder was weighed in portions of 2, 6, and 10 grams using the method described by Chikpah et al (2014), by increasing the sample dose based on the sideline laboratory toxicity test. The extraction was assessed by the method described by García et al (2012) and Shobowale, Ogbulie, Itoandon, Oresegun, and Olatope (2013). The extraction temperature was modified from constant temperature to 30, 46, and 60°C for each sample dose, to analyze crude extract on clotting activity and cheese manufacturing process.…”

Section: Methodsmentioning

confidence: 99%

Manufacture of fresh cheese using east African Calotropis procera leaves extract crude enzyme as milk coagulant

Abebe

Emire

2020

Food Science & Nutrition

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The present work was aimed to develop fresh cheese using the crude extract from calotropis procera leaves and thereby performed evaluating milk‐clotting activity and acute toxicity of crude extract. The extraction of coagulant was performed at 30, 45, and 60℃ using 2, 6, and 10 g of dried calotropis procera leaves, respectively. The highest yield of crude enzyme extract was acquired with 2 g and 30℃, and results were varied significantly (p < .05). The highest cheese yield and fastest clotting time were 17.89 kg cheese/100kg milk and 14:50 min acquired with 10 g C. procera powder at 60℃ extraction temperature, respectively. E. coli, total bacterial count, yeast, and mold microbial load detection were observed below the limit established by codex alimentarius. This study recommends that use of C. procera crude enzyme leaves extract can be used as alternate milk coagulant for production of fresh cheeses. Soft cheeses manufactured with calotropin enzyme as milk coagulant revealed no adverse effects of toxicity tested on albino rates to the highest dose.

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“…The plant is rich in many types of secondary metabolites, that found to have in-vitro antimicrobial properties 3,4,5 . Because most plants have medicinal potency 6 , recently their extracts have been developed and proposed for use in food as natural antimicrobials 7,8 .…”

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confidence: 99%

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2019

IJPSR

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Calotropis procera plant belongs to the Asclepiadaceae family. It is prescribed to treat bronchitis, asthma, cough, infections, cancer, ascites, intestinal worms, cutaneous diseases, eczema, leprosy and also aid to stimulate the immune system. The leaves of C. procera were investigated for their phytochemical and anti-microbial activity. Chemical screening of leaves ethanolic extract exhibited to contain active compounds like alkaloids, terpenoids, flavonoids, saponins, and reducing sugar. The antibacterial activity of C. procera leaves against four different bacteria revealed that the ethanolic extracts could kill only Staphylococcus aureus and Basillus subtilis, but more resisted by Pseudomonas sp., and Escherichia coli compare to the standard antibiotic streptomycin. GC-MS analysis of the ethanolic extracts indicated that the highest percentage in 100% ethanolic leaves extract belongs to camphene 6.22%, thebaine 7.59% dodecanoic acid 19.15%, and linolenic acid ethyl ester 14.87%, while the highest percentage in 70% ethanolic leaves extract belongs to hexa-hydro-farnesol 9.87%, gamolenic acid 12.71%, and linolenic acid ethyl ester 6.83%. This is the first phytochemical, and antibacterial study on C. procera leaves in Iraq.

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Phytochemical and Antimicrobial Evaluation of Aqueous and Organic Extracts of Calotropis procera Ait Leaf and Latex

Cited by 37 publications

References 9 publications

Effects of Medicinal Plant Extracts and Photosensitization on Aflatoxin Producing Aspergillus flavus (Raper and Fennell)

Effects of Medicinal Plant Extracts and Photosensitization on Aflatoxin Producing Aspergillus flavus (Raper and Fennell)

Manufacture of fresh cheese using east African Calotropis procera leaves extract crude enzyme as milk coagulant

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