Phytochemical, Antioxidant, Antimicrobial, and Protein Binding Qualities of Hydro-ethanolic Extract of Tinospora cordifolia

Abstract

Data Phytochemical, antioxidant, antimicrobial, and protein binding qualities of hydro-ethanolic extract of Tinospora cordifolia S1. Qualitative analysis of phytochemicalsSmall branches and stem bark extracts (petroleum ether, acetone and methanol) of TC were analyzed for the presence of various phytochemicals using the respective chemical tests as follows. S1.1. Test for glycosides0.5 mL extract was taken in a test tube, 0.2 mL of 10 % ferric chloride solution and (50 %) glacial acetic acid added. Few drops of concentrated sulphuric acid were added. A blue color production shows the presence of glycosides. S1.2. Tests for terpenoidsExtract was mixed with chloroform and a few drops of conc. H 2 SO 4 were added, shaken well and allowed to stand for some time. Red color appeared at the lower layer indicated the presence of steroids and formation of yellow colored layer indicated the presence of terpenoids. S1.3. Test for proteinsAn aliquot of 2 mL of extract was treated with one drop of 2% copper sulphate solution. To this, 1 mL of ethanol (90%) was added, followed by excess of potassium hydroxide pellets. Pink color in ethanol layer indicated the presence of proteins. S1.4. Test for amino acidsTwo drops of ninhydrine (5%) were added to 1 mL of extract. A characteristic purple color indicated the presence of amino acids. S1.5. Test for alkaloidsTwo millilitre of 1 % HCl was mixed with 0.1 gm of crude extract and heated slightly. After cooling Wagner's reagent and Mayer's reagent were added to it. The presence of buff colored precipitate indicated the presence of alkaloids. S1.6. Test for carbohydratesBenedict's reagents was mixed with the 1 mL of crude extract and slightly boiled, appearance of reddish brown precipitate indicated the presence of the carbohydrates. S1.7. Test for flavonoidsThe appearance of pink scarlet color when 1 mL of crude extract was mixed with few drops of concentrated HCl and Mg pellets indicated the presence of flavonoids. S1.8. Test for phenols2 mL of 2 % ferric chloride was mixed with the 1 mL of crude extract and the presence of bluegreen or black coloration indicated the presence of phenols.

Comparative Assessment of Phytochemicals, Antioxidant, and Antimicrobial Potential of Stem Bark and Small Branches of Buchanania cochinchinensis (Lour.) MR Almeida for Substitution in Ayurvedic Drugs

“…The BSA protein binds to acetylsalicylic acid and various extracts of SB and STB were performed. 1,3 The mean values of protein-binding constants are observed to be 2.31 ± 0.05 × 10 6).…”

“…4 The qualitative analysis of phytochemicals, viz., phenols, tannins, carbohydrates, saponins, alkaloids, flavonoids, furanoids, quinone, proteins, coumarins, steroids, and triterpenoids was done as per recent studies. 1,3 Quantitative analysis of phytochemicals was done by using direct mass study. [13][14][15][16][17]…”

“…against S. aureus and Escherichia coli was found. [2][3][4][5][6] Plant-derived natural products having poor antimicrobial and antifungal activities are reported to represent synergism against microbial pathogens when combined with various chemotherapeutic compounds.…”

Correlation between the antioxidant capacity of plasma and blood glucose level

“…Cinnamomum zeylanicum bark and fruits contain proanthocyanidins which are flavonoids. In the ethanolic extract of Tinospora cordifolia, there are main components such as tinocordioside, cordifolide A, palmatine, quercetin, βsitosterol, heptacosanol, and syringin (Kumar et al, 2018). One of the compounds that act as an antioxidant in Tinospora cordifolia is flavonoid quercetin.…”

Evaluation of Antioxidant Potential of Stem and Leaf Extracts of Himalayan Tinospora cordifolia Hook.f. & Thomson

“…During the last few decades, there has been an increasing interest of scientists in the study of medicinal plants and surveys on their traditional use all over the world. Although some eminent work on Tinospora cordifolia has been reported from some areas of the world like Angat, Bulacan, Philippines [16] and some from different regions of India like Mysore [10,11], Ranchi [13], Khandari Campus Agra [14,15], Bengaluru [18], Gwalior (MP) [19], Udupi (Karnataka) [20], Chitrakoot region (MP) [22], West Singhkhum (Jharkhand) [23], Hazaribagh, and Bokaro (Jharkhand) [24]. However, comparative reports on Himalayan Tinospora cordifolia stem and leaf extracts so far have not been investigated.…”