Phytochemical Composition and Antibacterial Activity of Hydroalcoholic Extracts ofPterospartum tridentatumandMentha pulegiumagainstStaphylococcus aureusIsolates

Abstract

Pterospartum tridentatum and Mentha pulegium are largely used in Portuguese folk medicine to treat several human disorders and inflammatory processes but without any consistent evidence for those beneficial pointed properties. Thus, the aim of the current work is to evaluate its benefits and phytochemicals related to those beneficial properties. A distinct polyphenol profile between P. tridentatum and M. pulegium was found. Taxifolin, myricetin, ginestin, ginestein, and ginestein derivatives, biochanin A-glucoside, and biochanin A were identified in P. tridentatum, whilst in M. pulegium the luteolin-7-rutinoside, diosmin, and apigenin and respective derivatives were most representative polyphenols. These variations had implications in the antiradical and antibacterial activity and the P. tridentatum exhibited the highest antibacterial activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus MSSA, which was mainly dose-dependent. This antibacterial activity seems to be related to high content of flavonols, flavones, and isoflavones, which can act synergistically with each other against this type of bacteria. Our results showed consistent evidence that Pterospartum tridentatum and Mentha pulegium are an important reservoir of phytochemicals with antiradical activity and antibacterial capacity and thus they might be used in a preventive way or in a combined pharmaceutical and antibiotic therapy against pathogenic bacteria.

Metabolic Profiling of Nine Mentha Species and Prediction of Their Antioxidant Properties Using Chemometrics

“…Various polypheonols were identified and quantified in the M. pulegium extracts. However, the content of rosmarinic acid (0.287 mg/g) was lower than in our findings [14]. Chemical profiling of the Mentha species has also been used as an additional marker for confirming the parental origin of hybrids of naturally occurring and cultivated species [15].…”

Enhancing oxidative stability of sunflower oil by supplementation with prickled broom (Pterospartum tridentatum) ethanolic extract

“…Slightly higher antioxidant activity was observed in aqueous P. tridentatum extracts, EC 50 = 42.97 mg/L (Luís et al., 2009) and EC 50 = 50 mg/L (Roriz et al., 2015). Contrarily, methanolic extract showed lower activity, with EC 50 of 150 mg/L (Aires, Marrinhas, Carvalho, Dias, & Saavedra, 2016). Beside solvent, other factors, such as the plant section (flowers, stems, or leaves) and the harvest period, may influence the antioxidant activity of P. tridentatum extract, as demonstrated by Coelho et al.…”

Flavonoid Profile of the Genista tridentata L., a Species Used Traditionally to Treat Inflammatory Processes

“…In vitro experiments corroborated the antioxidant properties, with human endothelial cells being protected from H2O2-induced oxidative injury, an effect attributed to isoquercitrin [9]. Flavonols, flavones, and isoflavones are highly abundant in the hidroalcoholic extracts of the plant and confer them a strong antibacterial activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus [10]. Finally, the polysaccharides present in the dried inflorescence of G. tridentata were also pointed as possible contributors to its health beneficial effects, namely through relevant anti-inflammatory activity [11].…”