Phytochemical content an <i>in vitro</i> toxicity of <i>Glycine soja</i> ethanol extract on the A549 Lung cancer line cell

Amaani, R.; Dwira, Surya

doi:10.1088/1742-6596/1073/3/032042

Cited by 12 publications

(6 citation statements)

References 15 publications

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“…The MTT assay was used to determine the cytotoxicity effect of the optimized NGC against MRC5 and A549 cell lines. The range of the cytotoxic effect was based on the National Cancer Institute and the Geran Protocol: IC 50 < 21.00 µg/mL (highly toxic), IC 50 of 21.00-200.00 µg/mL (moderately toxic), IC 50 of 201.00-500.00 µg/mL (weakly toxic), and IC 50 values >500.00 µg/mL (non-toxic) [48]. Table 8 showed that the optimized NGC had reduced cytotoxicity effects against both MRC5 and A549 when compared with the control (Gem + Cis alone) from very toxic (IC 50 < 1.56 µg/mL) to weakly toxic (IC 50 280.00 µg/mL) and moderately toxic (IC 50 = 46.00 µg/mL), respectively, after 72 h of treatment.…”

Section: In Vitro Drug Release Analysismentioning

confidence: 99%

Aerosolized Niosome Formulation Containing Gemcitabine and Cisplatin for Lung Cancer Treatment: Optimization, Characterization and In Vitro Evaluation

et al. 2021

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Gemcitabine (Gem) and cisplatin (Cis) are currently being used for lung cancer treatment, but they are highly toxic in high dosages. This research aimed to develop a niosome formulation containing a low-dosage Gem and Cis (NGC), as an alternative formulation for lung cancer treatment. NGC was prepared using a very simple heating method and was further optimized by D-optimal mixture design. The optimum NGC formulation with particle size, polydispersity index (PDI), and zeta potential of 166.45 nm, 0.16, and −15.28 mV, respectively, was obtained and remained stable at 27 °C with no phase separation for up to 90 days. The aerosol output was 96.22%, which indicates its suitability as aerosolized formulation. An in vitro drug release study using the dialysis bag diffusion technique showed controlled release for both drugs up to 24 h penetration. A cytotoxicity study against normal lung (MRC5) and lung cancer (A549) cell lines was investigated. The results showed that the optimized NGC had reduced cytotoxicity effects against both MRC5 and A549 when compared with the control (Gem + Cis alone) from very toxic (IC50 < 1.56 µg/mL) to weakly toxic (IC50 280.00 µg/mL) and moderately toxic (IC50 = 46.00 µg/mL), respectively, after 72 h of treatment. These findings revealed that the optimized NGC has excellent potential and is a promising prospect in aerosolized delivery systems to treat lung cancer that warrants further investigation.

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Section: In Vitro Drug Release Analysismentioning

confidence: 99%

Aerosolized Niosome Formulation Containing Gemcitabine and Cisplatin for Lung Cancer Treatment: Optimization, Characterization and In Vitro Evaluation

et al. 2021

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“…Red and Premium French LcCEs demonstrated IC 50 value of 1mg/ml concentration on A549 cells as compared to whole green LcCE (p < 0.0001 for both). Another study also showed comparable IC 50 values between Glycine soja extracts (from the same family as lentils) and cisplatin (broad-spectrum chemotherapy medicine) on A549 cells (Amaani and Dwira, 2018).…”

Section: Cytotoxicity Assaymentioning

confidence: 80%

Commercial Lentils (Lens culinaris) Provide Antioxidative and Broad-spectrum Anti-cancerous Effects

Kiran

Johnson

Mani

et al. 2020

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Background: Interest is growing surrounding functional foods, which provide health benefits in addition to nutritional value. In particular, there is a focus on pulse crops which contain high levels of polyphenolics, such as lentils. Methods: In this study, polyphenols were extracted from five L. culinaris varieties and characterized by both their antioxidant profile and cytotoxic activity. Result: Hulled varieties had a high content of secondary metabolites (>70 mg GAE/g), while all varieties demonstrated high antioxidant potential (1570-2020 Oxygen Radical Absorbance Capacity index). Furthermore, all extracts showed significant cytotoxicity against H9C2, HepG2, A549 and Calu-1 cancer cell lines. This recommends further investigation into the specific compounds present in L. culinaris, which could potentially be exploited for their anti-cancer activity.

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“…This unique compound was completely elucidated to be 5-(sec-butyl)-2ethylfuran-3-carboxylic acid. No reference for the nuclear magnetic resonance (NMR) data of compound (50) was available. This is the first report of the NMR spectrum and biological activity of the furan-type compound (50) produced by a marine Streptomyces sp.…”

Section: Streptomyces Spmentioning

confidence: 99%

“…Cont. IC 50 values in µg/mL are <21 µg/mL for strong, 21-200 µg/mL for moderate, and 201-500 µg/mL for weak cytotoxicity, with values >501 µg/mL as non-cytotoxic[50]. IC50 measurements in µM, values of 1-10 µM can be defined as very strong, 11-25 µM defined as strong, 26-50 µM defined as moderate, and 51-100 µM defined as weak cytotoxic.…”

mentioning

confidence: 98%

Secondary Metabolites from Marine-Derived Fungi and Actinobacteria as Potential Sources of Novel Colorectal Cancer Drugs

et al. 2022

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Colorectal cancer is one of the most common cancers diagnosed in the world. Chemotheraphy is one of the most common methods used for the pharmacological treatment of this cancer patients. Nevertheless, the adverse effect of chemotherapy is not optimized for improving the quality of life of people who are older, who are the most vulnerable subpopulation. This review presents recent updates regarding secondary metabolites derived from marine fungi and actinobacteria as novel alternatives for cytotoxic agents against colorectal cancer cell lines HCT116, HT29, HCT15, RKO, Caco-2, and SW480. The observed marine-derived fungi were from the species Aspergillus sp., Penicillium sp., Neosartorya sp., Dichotomomyces sp., Paradendryphiella sp., and Westerdykella sp. Additionally, Streptomyces sp. and Nocardiopsis sp. are actinobacteria discussed in this study. Seventy one compounds reviewed in this study were grouped on the basis of their chemical structures. Indole alkaloids and diketopiperazines made up most compounds with higher potencies when compared with other groups. The potency of indole alkaloids and diketopiperazines was most probably due to halogen-based functional groups and sulfide groups, respectively.

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Phytochemical content an in vitro toxicity of Glycine soja ethanol extract on the A549 Lung cancer line cell

Cited by 12 publications

References 15 publications

Aerosolized Niosome Formulation Containing Gemcitabine and Cisplatin for Lung Cancer Treatment: Optimization, Characterization and In Vitro Evaluation

Aerosolized Niosome Formulation Containing Gemcitabine and Cisplatin for Lung Cancer Treatment: Optimization, Characterization and In Vitro Evaluation

Commercial Lentils (Lens culinaris) Provide Antioxidative and Broad-spectrum Anti-cancerous Effects

Secondary Metabolites from Marine-Derived Fungi and Actinobacteria as Potential Sources of Novel Colorectal Cancer Drugs

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