Phytochemical Screening and Antibacterial Activity of Acorus calamus L. Extracts

Muchtaromah, Bayyinatul; Hayati, Alfiah; Agustina, Erna

doi:10.15575/biodjati.v4i1.4235

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…The plant extract of A. calamus however, showed zone of inhibition against E. coli at only the highest concentration of 200mg/ml with 11mm. The limited activity against E.coli was also reported by Muchtaromah et al (2019). The study by Maharjan et al (2013) showed no zone of inhibition against K, pneumoniae and P. aeruginosa, however our study showed zone of inhibition against it at higher concentration.…”

Section: Antimicrobial Activity Of Samples Extractssupporting

confidence: 71%

Antimicrobial Assessment and Phytochemical Screening of Medicinal Plants and Ganoderma lucidum.

Thapa

Maharjan

Tamang

et al. 2022

Int J Appl Sci Biotechnol

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Nepal has always relied on traditional medicinal plants, herbs, and mushrooms that are locally available to treat numerous diseases. This study thus aimed to explore the antimicrobial properties of the plants and fungi that are commonly used as traditional medicines. Antimicrobial properties of 5 plants (Curcuma caesia. Acorus calamus, Moringa oleifera, Terminalia chebula, and Tinospora cordifolia) and 1 fungus (Ganoderma lucidum) were screened against 4 ATCC bacterial culture (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae). The extracts were prepared using Soxhlet’s apparatus in two solvents, i.e. ethanol and distilled water. Out of which, distilled water of Acorus calamus had the highest percentage yield. All extracts showed antimicrobial properties against S. aureus. The highest potential was observed in the ethanolic extract of Curcuma caesia against S. aureus with the zone of inhibition of 22mm The ethanolic extract of Tinospora cordiofolia showed promising antimicrobial against gram-negative bacteria: E.coli, P. aeruginosa and K. pneumoniae with the zone of inhibition 15mm, 15mm and 14mm respectively. The distilled water extract of Acorus calamus showed the highest antimicrobial activity against P. aeruginosa with a zone of inhibition of 18mm. In the case of phytochemical screening, both the extracts of Tinospora cordiofolia showed the highest phytochemicals (Terpenoids, Saponins, Coumarin, Flavonoids, Alkaloids, and Tannins). The experiment confirmed the efficacy of selected plants and fungus extract as a natural antimicrobial against all the test organisms used, suggesting the possibility of employing them in novel drug development against the treatment of infectious diseases caused by the test organisms.

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Section: Antimicrobial Activity Of Samples Extractssupporting

confidence: 71%

Antimicrobial Assessment and Phytochemical Screening of Medicinal Plants and Ganoderma lucidum.

Thapa

Maharjan

Tamang

et al. 2022

Int J Appl Sci Biotechnol

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“… and β-Asarone were identified as the main compounds with antibacterial properties from A. calamus in all parts of the plant, roots, rhizomes, and essential oils. The essential oil from the tetraploid form of A. calamus rhizome contains about 95-96% β-Aaron as a component mainly [12,13].…”

Section: Accordingmentioning

confidence: 99%

Docking studies for screening antibacterial compounds of Red Jeringau (Acorus calamus L.) using Shigella flexneri protein as a model system

Dyas

Wijianto

2023

Acta.Chim.Asiana

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Alpha (a) and beta (β) asarone were identified as the main compounds of red Jeringau (Acorus calamus L.) and had antimicrobial properties. This study aimed to know these two compounds' antibacterial mechanism and toxicity prediction against the PBP 2 protein and 50S Ribosomal Protein of Shigella flexneri. Molecular docking protocol using PyRx device was performed with Exhaustiveness value= 106, grid x=38.738375, y=112.645792, z=46.926417 for PBP2, and grid x=71.721251, y=47.551601, z=9.663173 for 50S Ribosomal Protein. The molecular docking results on the α -Asarone compound obtained an affinity value of -5.7 kcal/mol for PBP2 and an affinity value of -5.6 kcal/mol for 50S Ribosomal Protein. In comparison, β-Asarone had an affinity value of -5.6 kcal/mol to PBP2 and an affinity value of -5.7 kcal/mol for 50S Ribosomal Protein. The α and β-Asarone affinity had better values than the control. Molecular docking of α and β-Asarone compounds results in ionic bonds to the TYR529 amino acid and polar bonds to the ASN552 amino acid of PBP2. However, only β-Asarone produces ionic bonds at the amino acid ILE17 and polar bonds at GLU13 from 50S Ribosomal Protein. Based on this study, the α and β-Asarone compounds were shown to have antibacterial activity by interfering with the permeability of the bacterial cell wall. Both compounds are also predicted to have carcinogenic and mutagen effects.

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“…Anti-oxidative and anti-inflammatory can be obtained from various types of plants, one of which is Acorus calamus L. It is a water plant that contains phytochemical compounds such as alkaloids, carbohydrates, flavonoids, tannins, and phenolics (Muchtaromah et al, 2019). With its content of phytochemical compounds, the ethanol extract of A. calamus L. rhizome can prevent the suppression of stressed rats' immune systems in normal rats.…”

Section: Introductionmentioning

confidence: 99%

Morpho-histological Study of the Rat Spleen Post-Induction of Sodium Nitrite and Acorus calamus L. Rhizome Ethanolic Extract

Amira,

Febriani,

Syukriah

et al. 2023

JRBA

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Sodium nitrite (NaNO2) is one of the most inorganic salts and is often used in processed meat products. Long-term consumption of sodium nitrite has been reported to cause of oxidative stress and spleen histological changes. Therefore, anti-oxidative effects are needed from natural ingredients such as Acorus calamus L. The study aims to determine the effect of A.calamus L. rhizome extract on anatomical structure and histological changes in the rat spleen induced with NaNO2. We used a completely randomized design with twenty-five male rats divided into 5 groups. Negative control (NC) was given 0.5% CMC for 40 days; positive control (PC) on days 1-15 was given NaNO2 (50 mg/kg BW); on days 16-30 given (50 mg/kg BW) NaNO2 + 0.5% CMC and on days 31-40 given 0,5% carboxy methyl cellulose (CMC). Treatment 1 (T1), Treatment 2 (T2), and Treatment 3 (T3) on days 1-15 were given NaNO2 doses of 50 mg; and; on days 16-30, groups T1, T2, and T3 were given NaNO2 + extract with each predetermined dose (250 mg/kg BW for T1, 500 mg/kg BW for T2, and 750 mg/kg BW for T3). For days 31-40 groups T1, T2, and T3 were given extracts with each predetermined dose. Results revealed that the extract of the A. calamus L. rhizome affects weight, length, and width of the spleen (P<0.05); and bleeding lesions; while having no noticeable effect (P<0.05) and having a noticeable effect on the area of the white pulp (P<0.05). Administration of ethanol extract of A. calamus L. at a dose of 250 mg/kg BW has an impact on improving spleen length; a dose of 500 mg/kg BW has an impact on the weight and length of the spleen; and a dose of 750 mg / kg BW has an impact on improving spleen length, but the size variation is still in the normal category. While extracts at doses of 250 and 500 mg/kg BW have a repair impact on tissue shrinkage in damaged white pulp.

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Phytochemical Screening and Antibacterial Activity of Acorus calamus L. Extracts

Cited by 4 publications

References 12 publications

Antimicrobial Assessment and Phytochemical Screening of Medicinal Plants and Ganoderma lucidum.

Antimicrobial Assessment and Phytochemical Screening of Medicinal Plants and Ganoderma lucidum.

Docking studies for screening antibacterial compounds of Red Jeringau (Acorus calamus L.) using Shigella flexneri protein as a model system

Morpho-histological Study of the Rat Spleen Post-Induction of Sodium Nitrite and Acorus calamus L. Rhizome Ethanolic Extract

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