Phytochemical Screening and Antibacterial Activity of  Hibiscus rosa - sinensis Leaf Extracts

Vastrad, Jyoti V.; Byadgi, Shameembanu A.

doi:10.20546/ijcmas.2018.703.384

Cited by 15 publications

(13 citation statements)

References 5 publications

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“…Phytochemical screening: The phytochemical evaluation of Datura stramonium shows the presence of alkaloids, tannins, saponins, glycosides and terpenoids; which matches with the previous literature [5,6] . The phytochemical evaluation of Hibiscus rosasinensis shows the presence of alkaloids, tannins, glycosides and carbohydrates; which matches with the previous literature [7,8] The phytochemical evaluation of Saccharum officinarum shows the presence of alkaloids, tannins, saponins, glycosides and terpenoids; which matches with the previous literature [9][10][11] . The percentage inhibition of haemolysis was calculated in UV-Visible spectrometry method at 560mn.…”

Section: Resultssupporting

confidence: 87%

In-Vitro Anti-Inflammatory Activity of Combined Extracts of Selected Plants

K¹,

B²,

Mounika³

et al. 2020

IJAPB

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Inflammation is a vital response provided by the immune system that ensures survival during infection and tissue injury. Non-Steroidal Anti-Inflammatory drugs (NSAIDs) are the most common medication used to treat inflammation. But these drugs may cause moderate to severe adverse effects upon long term use. Here, an attempt was made to use the herbal drug combinations to treat inflammation and to evaluate the best combination to treat inflammation. HRBC method was used in the present study to evaluate the antiinflammatory activity. The in-vitro results suggest that, the combination of Datura stramonium and Hibiscus rosasinensis inhibits the haemolysis of erythrocytes; so, the combination can be used to treat inflammation.

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Section: Resultssupporting

confidence: 87%

In-Vitro Anti-Inflammatory Activity of Combined Extracts of Selected Plants

K¹,

B²,

Mounika³

et al. 2020

IJAPB

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“…The presence of some phytoconstituents in the extract was studied by standard phytochemical methods. Phytochemical analysis of alkaloids, anthocyanins, flavonoids, polyphenols, saponins and tannins were performed according to the tests described in the literature [ 50 , 51 ]. Table 1 represents the tests conducted for confirming the presence of phytochemicals.…”

Section: Methodsmentioning

confidence: 99%

“… Phytochemicals Tests Reagents Results Reference Alkaloids Hager’s test Hager’s reagent Prominent yellow ppt 51 Polyphenols Ferric chloride test 2% FeCl 3 Blackish green coloration 50 Flavonoids Ammonia test 1% NH 3 Yellow color 51 Anthocyanin Ammonia test 2N HCl, NH 3 Purplish blue 50 Terpenoids Salkowski test 0.5mL CHCl 3 , 1mL Conc. H 2 SO 4 Reddish brown coloration at the interface 51 Tannins Lead acetate test 10% Lead acetate Bulky white ppt 51 Saponins Foam test Distilled water Persistent foam 51 …”

Section: Methodsmentioning

confidence: 99%

Application of green synthesised copper iodide particles on cotton fabric-protective face mask material against COVID-19 pandemic

Archana

Rajagopal

Krishnaswamy

et al. 2021

Journal of Materials Research and Technology

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Microorganisms cause variety of diseases that constitutes a severe threat to mankind. Due to the upsurge of many infectious diseases, there is a high requirement and demand for the development of safety products finished with antimicrobial properties. The study involves the antimicrobial activity of natural cotton coated with copper iodide capped with Hibiscus rosa-sinensis L. flower extract (CuI-FE) which is rich in anthocyanin, cyanidine-3-sophoroside by ultrasonication method. The coated and uncoated cotton fabric was characterised through XRD, SEM, AFM, tensile strength and UV-Visible spectroscopic techniques. XRD confirmed the formation of CuI particles, SEM showed that CuI-FE was prismatic in shape. The average size of CuI-FE particles was found to be 552.45 nm. Anti-bacterial studies showed copper iodide particles to be a potent antimicrobial agent. AFM images confirmed the rupture of bacterial cell walls in the presence of prismatic CuI-FE. In-vitro cytotoxicity investigation of CuI-FE was performed against cancer and spleen cell lines to evaluate the cell viability. Cytotoxicity analysis revealed the IC 50 value of 233.93 μg/mL in the presence of CuI-FE. Molecular docking study was also carried out to understand the interaction of CuI-FE with COVID-19 main protease. This paper has given an insight on the usage of CuI-FE coated on the cotton fabric that has proved to have strong inhibition against the nano ranged bacterial, cancerous cell line and a strong interaction with the COVID-19 protease.Such eco-friendly material will provide a safe environment even after the disposable of medical waste from the infectious diseases like influenza and current pandemic like COVID-19.

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“…Berdasarkan hasil uji analisis fitokimia, bunga sepatu (H. rosa sinensis L.) memiliki kandungan senyawa kimia seperti flavonoid, polifenol, tanin, saponin, alkaloid, minyak atsiri yang berguna dalam pengobatan dengan memiliki berbagai macam bioaktivitas antara lain antiinflamasi, antikanker, antifertilitas, antiviral, antidiabetes, antidepresan, diuretik, serta antifungal. [18][19][20][21] Cyanidin dan quercetin merupakan salah satu dari grup anthocyanin dan flavonoid yang mempunyai konsentrasi paling banyak pada bagian bunga tanaman bunga sepatu. Cyanidin dan quercetin tersebut memiliki aktivitas antifungal yang cukup bermakna terhadap berbagai fungi patogen karena mampu menghambat spora patogen, dan digunakan sebagai pengobatan jamur patogen.…”

Section: Pendahuluanunclassified

“…Cyanidin dan quercetin tersebut memiliki aktivitas antifungal yang cukup bermakna terhadap berbagai fungi patogen karena mampu menghambat spora patogen, dan digunakan sebagai pengobatan jamur patogen. 19,22 Penelitian sebelumnya oleh Krista dan Arundina 22 menunjukkan bahwa ekstrak bunga sepatu (H. rosa sinensis L.) dengan konsentrasi hambat minimal (KHM) sebesar 62,5% mampu menghambat pertumbuhan koloni C. albicans setelah diuji pada beberapa konsentrasi. 22 Berdasarkan latar belakang yang telah diuraikan, maka penulis merasa perlu untuk melakukan penelitian guna mengetahui pengaruh perendaman ekstrak bunga sepatu (H. rosa sinensis L.) terhadap pertumbuhan koloni C. albicans pada plat gigi tiruan resin akrilik.…”

Section: Pendahuluanunclassified

Pengaruh Perendaman Ekstrak Bunga Sepatu (Hibiscus rosa sinensis L.) terhadap Pertumbuhan Candida albicans pada Plat Resin Akrilik

Syaula

Antari

Purbaningrum

2021

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Denture plate materials such as acrylic resin can induce adhesion of Candida albicans. Therefore, acrylic resin needs to be immersed in disinfectant. However, disinfectant can change its physical and mechanical properties, hence an alternative material is needed, such as hibiscus flower (Hibiscus rosa sinensis L.) due to its antifungal activity. This study was aimed to identify the effects of hibiscus flower extract and its concentrations towards the growth of C. albicans on acrylic resin plates. This was an experimental and laboratory study using the post-test only with control group design. Acrylic resins were immersed in suspension of C. albicans, then were divided into four groups, as follows: 62.5% and 75% hibiscus flower extract (group I and II), positive control (sodium hypochlorite), and negative control (sterile aquadest). Acrylic resins were cultured and incubated on SDA media for 24 hours then the number of colonies were calculated. The results showed that C. albicans colonies in the treatment groups I, II, negative control, and positive control were 495 CFU/ml, 571.25 CFU/mL, 1175 CFU/mL, 23.125 CFU/mL respective-ly. The Kruskal-Wallis test showed significant differences in number of colonies of C. albicans (p<0.05) among all groups The post hoc Mann-Whitney test showed that all groups were significantly different, except for treatment groups I towards II. In conclusion, extract of hibiscus flower (H. rosa sinensis L.) affected the growth of C. albicans on acrylic resin plates.Keywords: hibiscus flower; Hibiscus rosa sinensis L.; Candida albicans; acrylic resin Abstrak: Adanya bahan plat basis gigi tiruan seperti resin akrilik dapat memicu perlekatan C. albicans; oleh karena itu, resin akrilik perlu direndam dalam larutan desinfektan. Namun, larutan desinfektan dapat mengubah sifat fisik dan mekanik dari akrilik sehingga diperlukan adanya bahan alternatif, antara lain bunga sepatu (Hibiscus rosa sinensis L.) yang memiliki aktivitas antifungal. Penelitian ini bertujuan untuk mengetahui pengaruh perendaman ekstrak dan konsentrasi bunga sepatu terhadap pertumbuhan C. albicans pada plat resin akrilik. Jenis penelitian ialah eksperimental laboratorik dengan post-test only with control group design. Resin akrilik direndam dalam suspensi C. albicans, Terdapat empat kelompok perlakuan yaitu ekstrak bunga sepatu 62,5% dan 75%, kontrol positif (sodium hipoklorit), dan kontrol negatif (akuades steril). Resin akrilik dikultur dan diinkubasi pada media SDA selama 24 jam, kemudian jumlah koloni C. albicans dihitung. Hasil penelitian ini menunjukkan jumlah koloni C. albicans kelompok perlakuan I, II, kontrol negatif, dan positif sebanyak 495 CFU/ml, 571.25 CFU/mL, 1175 CFU/mL, 23.125 CFU/mL secara berurut. Uji Kruskal-Wallis menunjukkan perbedaan jumlah koloni C. albicans yang bermakna (p<0.05) antar semua kelompok. Uji post hoc Mann-Whitney menunjukkan semua kelompok berbeda bermakna, kecuali kelompok perlakuan I dengan II. Simpulan penelitian ini ialah ekstrak bunga sepatu (H. rosa sinensis L.) berpengaruh terhadap pertumbuhan C. albicans pada plat resin akrilik.Kata kunci: bunga sepatu; Hibiscus rosa sinensis L.; Candida albicans; resin akrilik

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Phytochemical Screening and Antibacterial Activity of Hibiscus rosa - sinensis Leaf Extracts

Cited by 15 publications

References 5 publications

In-Vitro Anti-Inflammatory Activity of Combined Extracts of Selected Plants

In-Vitro Anti-Inflammatory Activity of Combined Extracts of Selected Plants

Application of green synthesised copper iodide particles on cotton fabric-protective face mask material against COVID-19 pandemic

Pengaruh Perendaman Ekstrak Bunga Sepatu (Hibiscus rosa sinensis L.) terhadap Pertumbuhan Candida albicans pada Plat Resin Akrilik

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